Biochemical characterization of the protein encoded by rice osRACD gene

A recombinant plasmid pET-racd was first constructed by cloning osRACD,the development-regulating gene that controls photoperiod fertility transformation in the photoperiod sensitive genic male sterile rice Nongken 58S,into a prokaryote expression vector pET28a(+).It was then transformed into E.Coli BL-21.Cutting with thrombin of the fusion protein extracted from transformants and PAGE separation yielded pure osRACD protein,which was further concentrated using ultrafiltration and renatured using glutathione oxidation/reduction refolding system for later functional study.As demonstrated by in vitro functional assay,the osRACD protein expressed in E.Coli B-21 shows remarkable activity in binding GTP specifically and hydrolyzing it.     

Function analysis of a semi-dwarf sdg in rice with near isogenic lines

The physiological phenotype of the rice semi-dwarf mutant,sdg,was characterized in details using a pair of near isogenic .Neither gibberellin*deficient nor gibberellin-insensitive is characteristic of the sdgphenotype.Byanalyzing the secretion of aamylas and the promotion of stem growth caused by eogenous gibberellin(GA),the sdg plant was found to have decreased sensitivity to the GA at the seedling stage. The dwarfism stature of the sdg mutant can be attributed to the shortened internodes. Increase of the cell sults indicate the protein encded by the wild type gene of sdg may be a regulator for cell elogation.     

A novel audio watermarking scheme using multiscale wavelet modulation

A novel audio watermarking scheme to embed robust and inaudible watermarks for the purpose of copyright protection is proposed. The key innovation is to add time-frequency redundancy into watermark signals by multiscale wavelet modulation. In order to maximize the watermarking strength within perceptual constraints, the signals synthesized from different scales are masked using a frequency auditory model, respectively, and then intergrated to form the final watermark signal. The detection structure is built using the redundancy in watermark signals, and the performance is further enhanced by modeling the statistical behaviors of wavelet coefficients as generalized Gaussian distribution. The use of original audio signal is not required in watermark detection. The experimental results show that our approach can achieve not only good transparency but also satisfying robustness to common audio manipulations.     

Relationship between the exposure dose of high intensity focused ultrasound and the heated necrosis element

Heated necrosis element of tissues is the basis for treating tumor by high intensity focused ultrasound (HIFU), so study on the relationship between the HIFU exposure dose and the heated necrosis element of tissues is of great significance for both HIFU clinical applications and basic research. Using Pennes and Rayleigh formulae the relationship between the exposure dose of an annular focused transducer and the heated necrosis element (I.e. Biological focused region) in a bovine liver in vitro at a depth of 2 cm is simulated in an exposure dose range with radiation sound intensity of 7000～25400 W/cm2 and exposure time of 0～20 s. The theoretical results appear to be in quite good agreement with the experimental ones. It is shown that on the basis of existing theories the heated necrosis element produced by HIFU exposure can be predicted. The deviation of the theory from the experiments under higher exposure dose has also been discussed.     

Detection of the translational oscillations of the Earth''''s solid inner core based on the international superconducting gravimeter observations

Before about 100 years, the layered structures of the Earths body and the existence of the liquid core had been found, then the solid inner core and detailed structures in the interior of the Earth are demonstrated, it proves that the satisfied achievements had been obtained in recogni-tion of the Earths interior structures. In recent years, with the increasing requirements of the basic sciences and space techniques to geodynamics of the Earths interior, the theoretical study and practical de…     

组织学习协同性评价模型设计与应用

在分析组织系统学习协同要素的基础上，运用协同学理论构建组织学习的协同性评价指标体系和评价模型，通过实证研究对一些代表性企业进行协同性评价，以探索提升组织学习能力和效果的途径。     

重置变结构神经网络及其在风险投资项目评估中的应用

基于神经网络的结构直接影响到网络性能的优劣,进而影响其推广使用,尝试将重置算法应用于经典BP神经网络的结构优化,研究了重置算法中最佳重置时间的性质,提出一种重置变结构经典BP神经网络。通过实例,将重置变结构经典BP神经网络应用于风险投资的项目风险评估中,并与经典BP神经网络的实验结果进行对比。结果表明,重置算法的引入有效地解决了神经网络结构的优化问题。     

BA模型的三种扩展

无标度网络的发现，使人类对于复杂网络的认识进入了一个新的天地．BA模型是对于这种新认识的第1个抽象模型．由于考虑了系统的成长性和连接的择优性，BA模型给人们带来了很多启发．但是BA模型的两个基本假定，对于解释许多现实中的现象来说过于简单，与现实的网络还有较大的距离．所以，作者试图对BA模型进行扩展，即根据现实中的网络，增添某些假定，并通过计算机模拟进行观察，以便进一步探索复杂网络系统的规律．对BA模型的扩充考虑了3个因素：择优选择的成本；边的重新连接；网络的初始状态．实验结果表明，在考虑了这些因素之后，BA模型的基本结论并没有变化，它们的影响在于演化过程的某些方面，例如在某些极端条件下的演变趋势等．     

机会约束下的均值-VaR组合投资问题

结合均值一方差模型和机会约束模型提出在允许卖空时的机会约束下的均值-VaB模型，它是以期望收益率与置信水平为导向的．在假设投资收益率服从正态分布的条件下，建立了其数学模型，讨论了最优解的存在性与唯一性，得到了最优解的解析表达式，并用Matlab语言给出求解程序，最后举例予以说明并验证了两个重要结论．     

不确定条件下的flow shop问题的免疫调度算法

生产调度是企业生产过程中一个重要部分，而且实际的生产过程中会存在各种各样的不确定性，针对不确定条件下的flow shop（流水车间作业）调度问题，采用模糊数学的方法来处理数据的不确定性，在基于模糊规划理论的基础上建立了相应的调度模型，并结合免疫算法的特点，提出了解决此类问题的模糊免疫调度算法．通过仿真试验，证明了模型和算法的可行性和有效性．