取代氧化吡啶气相质子化能的分子轨道法研究

在LCAO-MO-AM1水平上计算了取代氧化吡啶的气相质子化能.发现质子化能大小与取代氧化吡啶中发生质子化的氧原子上净电荷分布有较好的线性关系.△H=1228.73kJmol~(-1),qo/e为-158.98,相关系数为0.995.     

移动通信双基站工作时接收场强的计算及模拟

对开阔地在双基站设置时的场强分布进行了探讨和计算，计算结果与实测数据较好符合。并通过计算机模拟，得到了有关电磁波在自由空间的传播损耗，大地表面的反射系数及相位等特性参数。     

γ-Al_2O_3上CO和CO_2吸附表面体的原位红外光谱研究

本文运用原位红外光谱研究了在γ—Al_2O_3上CO、CO_2的吸附及其与H_2或H_2O的共吸附。观测到不同情况下HCOO~-的生成规律及其随温度变化的行为。在(CO+H_2)的共吸附中观察到HCO_3~-向HCOO~-表面体的转变。在(CO+H_2O)共吸附中,HCOO~-不经HCO_3~-的中间步骤而直接生成。在CO_2的吸附中,检测到两种热稳定性不同的表面HCO_3~-体。而(CO_2+H_2)共吸附中也观察到了HCOO~-的形成。研究了表面甲酸体在真空中热分解以及它同H_2或H_2O的反应,证实了表面甲酸体更易于同H_2O反应。这三种情况下甲酸体显著反应温度分别约为400℃、300℃、100℃。     

固态烧结法合成新化合物Sr_2CaMoO_6

本文用固态烧结法合成了新化合物Sr_2CaMoO_6,并通过x射线衍射的测定,确定Sr_2CaMoO_6属正交晶系,其点阵常数为:a=8.158;b=5.733;c=5.835。     

Dynamics of Drosophila embryonic patterning network perturbed in space and time using microfluidics

Biochemical networks are perturbed both by fluctuations in environmental conditions and genetic variation. These perturbations must be compensated for, especially when they occur during embryonic pattern formation. Complex chemical reaction networks displaying spatiotemporal dynamics have been controlled and understood by perturbing their environment in space and time. Here, we apply this approach using microfluidics to investigate the robust network in Drosophila melanogaster that compensates for variation in the Bicoid morphogen gradient. We show that the compensation system can counteract the effects of extremely unnatural environmental conditions--a temperature step--in which the anterior and posterior halves of the embryo are developing at different temperatures and thus at different rates. Embryonic patterning was normal under this condition, suggesting that a simple reciprocal gradient system is not the mechanism of compensation. Time-specific reversals of the temperature step narrowed down the critical period for compensation to between 65 and 100 min after onset of embryonic development. The microfluidic technology used here may prove useful to future studies, as it allows spatial and temporal regulation of embryonic development.     

Expression,purification and spectra characterization of neuroglobin

The expression, purification and spectra characterization of recombinant human neuroglobin （NGB） are reported. The pET3a plasmid with the gene of NGB was transformed to E. coli BL21 （DE3） plys cells and expressed in TB culture medium. The results indicated that the expression amount of NGB is about 10 percent of the total protein in cells. The NGB protein was purified by ammonium sulfate precipitation, DEAE-Sepharose anion exchange column, Hiload 16/60 superdex 75 size exclusion chromatography and a Hiprep 16/10 Q FF anion exchange column, and a red soluble protein was obtained which showed a single band in electrophoresis. Electrospray ionization mass spectrometry （ESI-MS） showed that its molecular weight is 16930.0 Da. UV-spectra indicated that the reduced NGB has a strong absorption peak at 425 nm, and two weak peaks at 531 and 559 nm, which can be assigned to γ, β and α bands of porphyrin, respectively, and the oxidized NGB has a strong absorption peak at 413 nm which corresponds to the transition of π electrons in the porphyrin ring. The fluorescence maximal excitation wavelength is at 281 nm and its maximal emission wavelength is at 338 nm. CD spectra indicated that its secondary structure is a typical α helix, and has a positive peak at 410 nm induced by heme, The NGB protein is stable when the pH is higher than 4.     

金属薄膜与萃取复型电镜照片上测量球状第二相误差分析

本文通过理论和实验数据分析表明:在萃取复型和金属薄膜电镜照片上定量测量球状第二相的加权平均直径时,存在较大误差。文章同时给出了两种误差的大小和它们之间的关系。     

电网无功电流检测原理分析及实现方案

通过对电网无功电流的分析,提出电网无功电流的一种检测方法及实现方案。采用单片集成采样保持器来实现每周波无功电流峰值的采样与保持。采样信号可用来表征电网变化情况或作为电网无功功率自动补偿中的控制信号。     

钢筋混凝土悬臂深梁的抗剪研究

本文通过试验和非线性有限元分析,对悬臂深梁的抗剪性能和互剪破坏特征等问题进行了研究,并运用斜压场理论结合试验和有限元分析结果提出悬臂深梁抗剪强度的计算公式。