C－isotope composition and significance of the Sinian on the Tarim plate

102 samples of limestones,mudrocks shales and tillites from the Sinian in Kuluketage have been analyzed ,Four formations in the Sinian belong to glacial deposits in which the differences in δ^13C are distinct (-14.7‰- 4.2‰),Distinctly negative δ^13C excursions for three times support the viwe of three glacial deposits in this area suggested by previous authors,except for the durations of them ,δ^13C values are generally positive in the Beiyixi Formation of lower part of the Lower Sinian ,representing probably the rock records of a continental rift related to break-up of the Rodinian supercontinent,There are three times of distinct excursions of δ^13C curve in all strata,The first one is from positive values at the bottom to about -5‰ at the top of the Beiyixi Formation ,The second one,from 0‰-3‰ in the Arletonggou Formation of upper part of the Lower Sinian to -3.4‰---14.4‰ in the Teruiaiken Formation.The third one,from positive values in the Zhamoketi Formation of lower part of the Upper Sinian to negative values in glacial varves at the top of the Hankeerqiaoke Formation.     

Measurements of leucocyte membrane elasticity based on the optical tweezers

A 1-μm in diameter polystyrene bead coated with lectin is trapped by optical tweezers whics is formed by a focused laser beam (740nm).A leucocyte adhered to the bottom of sample cell is chosen to close to the trapped bead.When their stable combination is confirmed,the leucocyte is displaced by moving the sample stage.A“tether”is then formed between the trapped bead and the membrane.The force acting on the tether is measured by a detector equipped on the optical tweezers system.The deformation of the membrane is diverse in different contact conditions.When the contact area is small,the tether is very thin and the force on it is 7.4pN(10^-12N),while the tether is much thicker under a larger contact area and its corresponding force is about 14pN.It is presumed that the latter is related to the deformation of cytoskeleton.     

Real time observation of the photocleavage of single DNA molecules

A method for real time observation of photo-cleavage of stratched λDNA at single molecular level by a fluorescent microscope coupled with CCD is developed.DNA molecules stained with YOYO-1 are stretched by the mo-lecular combing technique and fixed on a modified slide.Then the Process of Photocleavage and relaxation of DNA under radiation of blue light is observed.We speculate that the conformation change of stretched DNA and the effect of water are likely to facilitate the effect of YOYO photocleav-age DNA molecules.The photocleavage effect of YOYO for stretched DNA may be useful to study DNA elasticity,cancer research as well as the interaction between DNA and dyes.     

Efficient expression of human factor Ⅸ cDNA in liver mediated by hydrodynamics-based plasmid administration

The transfection of plasmid DNA into mammalian cells is an indispensable tool in the study of gene transfer and gene function. Since the original report in 1990 on the successful expression of a reporter gene in muscle[1], plasmid DNA injection has been widely used to mediate gene transfer study. As a gene transfer vector, naked DNA has many obvious advantages. It is easy and cheap to be prepared and more safe after transfection. But the plasmid mediated gene expression is low because of t…     

A complete sequence and comparative analysis of a SARS-associated virus(Isolate BJ01)

Severe Acute Respiratory Syndrome (SARS) is a newly identified infectious disease[1—5]. The global outbreak of SARS has been threatening the health of people worldwide and has killed 353 people and infected more than 5462 in 27 countries, as reported by WHO on April 29, 2003 (http://www.who.int/csr/sarscountry/en). Although it has been recognized that a variant of virus from the family of coronavirus might be the candidate pathogen of SARS[1—5], its identity as the unique pathogen sti…     

A new species of Gobiconodon （Triconodonta, Mammalia） and its implication for the age of Jehol Biota

A new species of Gobiconodon is found from the Yixian Formation of western Liaonlng, China. The new taxa,G zofiae sp. nov., has a confluent opening for branches Ⅱand Ⅲ of the trigeminal nerve on the anterior lamina of the petrosal. G zofiae sp. nov. is similar to Repenomamus in having an ossified Meckel‘s cartilage connecting the lower jaws and ear region. The new species, with enlarged I^1/I1, posteriorly located infraorbital foramen and four mental foramina,distinctly differs from the other species of Gobiconodon. The new material indicates that Gobiconodon has four, not five,upper molariforms. The presence of Gobiconodon in Jchol Biota makes it possible to correlate Jehol Biota with faunas in eastern Asia and North America, and suggests the age of the Yixian Formation to be Early Cretaceous.     

在工科数学教学中加强数学应用意识教学的思考

阐述了加强数学应用意识教学的重要性,教师是加强数学应用意识教学的关键,加强数学应用意识教学应该适当增加数学实验与数学建模内容,在数学教学中加强对人的发展作用。     

Implementation of the finite deformation plastic theory-Tensor derivative and metal forging simulation

It has been shown that the traditional small deformation plasticity can be transplanted to the finite deformation plasticity by using the logarithmic strain measure only. In this way, the mathematical problem of how to find the derivative of a tensor function arises. In this paper, a general method for finding the derivative of a tensor function is proposed. Several metal forging examples are given.During the calculation, the mesh regeneration is not even invoked.     

A model for assessing effects of climate change on runoff in China

A model is established for assessing the effects of climate change on runoff in China based on the land surface parameterization scheme variable infiltration capacity (VIC). The entire area of China is represented by 2604 cells with a resolution of 60 km×60 km for each cell. Forcing data, soil and vegetation parameters needed by the VIC model for the entire area of China are prepared. Daily forcing data, which are obtained from 740 stations between 1980 and 1990, are interpolated to the 60 km×60 km grid system. The VIC model is run on every grid cell over the whole China, and a routing scheme is run offline with daily input of surface runoff and drainage from the VIC to get hydrograph at basin outlets. The spatial patterns of simulated runoff and mean annual precipitation are consistent very well. The results of monthly streamflow simulations over the Huaihe and Weihe River basins indicate that there is a good agreement between the observed and simulated values, and also initially indicate the rationality and feasibility of the evaluation model.     

A new method to dynamically simulate groundwater table in land surface model VIC

Soil moisture plays an important role in water and energy balance in land-atmospheric interaction, but is impacted directly by the groundwater table. Dynamic variation of the groundwater table can be described mathematically by a moving boundary problem. In this paper, the moving boundary problem is reduced to a fixed boundary problem through a coordinate transformation. A new model of groundwater table simulation is developed using the mass-lumped finite element method and is coupled with the land surface model of Variable Infiltration Capacity (VIC). The simulation results show that the new model not only can simulate the groundwater table dynamically, but also can evade the choice of water table depth scale in computation with a low computation cost.