Sequencing of phosphopeptides sulfonated by 4-sulfophenyl isothiocyanate using post-source decay matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

Phosphorylation/dephosphorylation is probably the most common and important reversible post-translational modification of proteins. Analyzing the functional effects of phosphorylation is helpful for understanding the biological functions of proteins. Identification of the phosphorylation sites of phosphorylated protein is a prerequisite for research on phosphorylation. In this work, an effective and simple method of identification of protein phosphorylation sites has been developed. Phosphopeptides were selectively enriched with immobilized metal affinity chromatography (IMAC) and subsequently chemically modified by 4-sulfophenyl isothiocyanate, and then the chemically modified phosphopeptides were sequenced with post-source decay (PSD) matrix-assisted laser desorption/ionization (MALDI) time-of-flight mass spectrometry for detecting phosphorylation sites. The charge of derivatization by 4-sulfophenyl isothiocyanate introduces a negative sulfonic acid group at the N-terminus of a peptide, and enables the selective detection of only a single series of C-terminal y-type ions. This chemically assisted method greatly simplifies the extremely complex pattern of PSD fragment ions and makes the PSD spectra more easier to be interpreted. The phosphorylation sites of a synthesized model phosphopeptide and human c-myc protein have been successfully identified by this method.     

Gene flow from genetically modified herbicide-resistant rapeseed to cruciferous weeds

The sexual compatibility between genetically modified (GM) glyphosate-resistant rapeseed variety Q3 (Brassica napus L.) and 5 cruciferous weeds is studied through the observation of fluorescence microscopy and cross-fertility after manual pollination. The results indicated that Q3 (as male) was highly incompatible with Thlaspi arvense L., Capsella bursa?pastoris (L.) Medic, Cardamine hirsuta L. and Rorippa palustris (L.) Besser (as female). Fluorescence microscopic observation showed that growing of pollen tubes terminated on the stigma surface or at the upper 1/3 part of the style. However, B. juncea×Q3 was compatible, and the compatibility index was 1.65. Under the neighboring growth and natural pollination conditions, the rates of gene flow from Q3 to T. arvense, C. bursa-pastoris, C. hirsute and R. palustris were all 0, while it was 0.86% for B. juncea. These results indicate that there is difference in the rate of gene flow between GM rapeseed and cruciferous wild weeds, and frequency of gene flow is highly correlated with sexual compatibility.     

LBS中理想的移动台位置更新策略

位置服务（LBS）的许多应用需要记录用户的历史轨速，现有的LBS系统开销比较大，降低了系统的容量。针对这些缺点，提出了一种理想的移动台位王更新策略，通过把用户分成2类，并依据该分类生成了2个动态改变用户定位频率的算法，结合用户的运动状态和用户指定的触发定位距离以及最大定位间隔时间来改变用户定位频率，使得记录下来的用户历史轨迹更加准确，同时提高了移动台位置更新的灵活性。     

基于模拟电感的多涡卷混沌电路实验研究

在蔡氏电路中,利用模拟电感电路代替实际电感,进行了混沌及多涡卷混沌吸引子实验研究,在示波器上观察到了较好的波形.实验表明,将模拟电感应用在振荡电路中,电路不但容易起振,而且参数调节非常方便.     

解读克里普克的“必然观”

克里普克首先将必然性、先验性区分开。然后借助“可能世界”给出了对“必然”的重新界定,认为“必然”是“不可能不”。“可能世界”是无逻辑矛盾的世界。因而其必然是逻辑的、终极的必然。克里普克的其他思想,如历史因果命名理论、本质理论无不与这种必然观密切相关。克里普克追求终极的必然,但他并未十分清晰地区分不同层次的必然,因而产生了一些混淆。     

大孔吸附树脂对砀山酥梨汁色素的吸附研究

文章比较研究了4种大孔吸附树脂对砀山梨汁色素的吸附性能,并选择XAD16HP树脂在不同温度、不同料液比下,对其进行了吸附动力学实验。结果表明,XAD16HP的吸附等温线符合Freundlich模型,但不符合Langmuir模型,它对色素的吸附可能是多分子层的;XAD16HP的静态吸附速率曲线符合Lagergren一级速率方程,且吸附速率受颗粒内扩散的控制。     

基于资源基础理论的物流外包战略决策

根据资源基础理论,物流外包是一种战略选择,可以弥补企业在物流资源和能力上的缺陷。企业实施物流外包战略,可以减少在物流设备、物流管理和物流运作上的资本投入,从而把有限的资源投在核心能力和核心业务上,在降低机会成本和投资风险的同时,增强企业的核心能力和核心业务。     

P1波在饱和土层和建筑基础界面的反射与透射

考虑了土颗粒、孔隙流体的压缩性及孔隙流体与土骨架之间的粘性耦合作用,采用修改的Biot模型分析入射P1波在饱和土体和建筑基础界面的反射与透射,得到了反射系数与透射系数的一般计算式,并对反射系数、透射系数随入射角、频率的变化规律进行了数值模拟.结果表明,入射角是影响反射和透射系数的主要因素.     

自适应小波神经网络在故障诊断中的应用

为提高传统BP神经网络在故障诊断中的效率,提出用小波神经网络加以改进.采用动量法和学习率自适应调整结合的网络训练算法对小波网络的初始参数进行设置,提出自适应小波神经网络的故障诊断方法,详述其诊断原理,并结合实例证实了该方法应用于故障诊断的有效性.     

风险投资项目价值评估的一种实物期权方法

首先分析了风险投资项目的期权特性,把风险投资项目看作是由一系列欧式期权构成的复合期权;然后提出了适用风险投资项目价值评估的一种实物期权模型及算法,从而对传统的净现值(NPV)法忽视项目灵活性价值的缺陷进行了修正·最后给出了一个算例·