Exocytotic fusion is activated by Rab3a peptides.

Studies of intracellular traffic in yeast and mammalian systems have implicated members of the Rab family of small GTP-binding proteins as regulators of membrane fusion. We have used the patch clamp technique to measure exocytotic fusion events directly and investigate the role of GTP-binding proteins in regulating exocytosis in mast cells. Intracellular perfusion of mast cells with GTP-gamma S is sufficient to trigger complete exocytotic degranulation in the absence of other intracellular messengers. Here we show that GTP is a potent inhibitor of GTP-gamma S-induced degranulation, indicating that sustained activation of a GTP-binding protein is sufficient for membrane fusion. We have found that synthetic oligopeptides, corresponding to part of the effector domain of Rab3a, stimulate complete exocytotic degranulation, similar to that induced by GTP-gamma S. The response is selective for Rab3a sequence and is strictly dependent on Mg2+ and ATP. This suggests that sustained activation of a Rab3 protein causes exocytotic fusion. The peptide response can be accelerated by GDP-beta S, suggesting that Rab3a peptides compete with endogenous Rab3 proteins for a binding site on a target effector protein, which causes fusion on activation.     

“编译技术”试题库及试卷自动生成系统的研究

本文阐述了高等工科院校计算机专业“编译技术”课程试题库建立的必要性、试题库设计的理论基础及其软件实现原理．对比了本系统与一般试题库的不同与特点     

福州盆地南北港冲淤规律与洪涝灾害对策

本文探讨了福州盆地南北港冲淤规律，近年来北港洪水分流量呈上升趋势，洪峰对北港的压力加大，文中还对福州城防洪涝灾害的措施等方面作了研究。     

圣母圣心会呼和浩特传教述论

圣母圣心会在呼和浩特传教分晚清时期、民国时期。晚清时期，圣母圣心会传教士把传教的重点放在乡村，在呼和浩特无所作为。进入民国后，调整传教手段，迁址主教府，兴办医院、学校等公益事业。取得了一定效果。     

基于虚拟仪器的异步串行通信协议的检测方法

在分析基于RS－232C标准的串行异步通信原理的基础上，讨论了利用RVO2100虚拟数字示波器检测串行异步通信协议的方法，并与传统检测方法进行了比较。     

WebGIS实现方法与应用研究

在互联网高速发展的时代，基于WWW的地理信息系统应运而生。主要介绍了WebGIS的发展历程、技术特点和系统组织结构，并结合一个实际系统对其具体实现过程作了较详细的阐述。该系统构架在开发过程中取得了较好的效果。     

Resource Search in Unstructured Peer-to-Peer System Based on Multiple-Tree Overlay Structure

We propose a multiple-tree overlay structure for resource discovery in unstructured P2P systems. Peers that have similar interests or hold similar type of resources will be grouped into a tree-like cluster. We exploit the heterogeneity of peers in each cluster by connecting peers with more capacities closer to the root of the tree. The capacity of a peer can be defined in different ways （e.g. higher network bandwidth, larger disk space, more data items of a certain type etc.） according to different needs of users or applications.     

Nanostructured Functional Thermoplastic Polymeric Materials Based on the Molecular Control of the Blending

1 Results The development of the concepts of nanotechnology has given an important impact on the design of new polymer based materials which are in most cases characterized by a multiphase morphology. When at least one phase has nanometric dimension(s) the system can be considered as a nanocomposite where the interface is not only determining for the adhesion but also may play a role in some bulk properties. Indeed in nanostructured multiphase solids the interface is significant as a bulk component. The...     

The Three-component One-pot Synthesis of 4,6-Diarylpyrimidin-2(1h)-ones and 9-Phenyl-8-oxa-10,12-diaza-tricyclo[7.3.1.0~(2,7)]trideca-2(7),3,5-trien-11-one

1 Results Pyrimidinones (PMs) are a class of important heterocycles which have been well documented throughout the literature due to their biological importance. They exhibit a wide range of pharmaceutical and therapeutic properties[1].A rapid and efficient one-pot method for the synthesis of 4,6-diarylpyrimidin-2(1H)- ones and related heterocycles is described.The condensation of acetophenone derivatives,aldehydes and urea in the presence of sulfamic acid was employed to synthesize a variety of pyrimid...