A mouse model of human L1 retrotransposition

The L1 retrotransposon has had an immense impact on the size and structure of the human genome through a variety of mechanisms, including insertional mutagenesis. To study retrotransposition in a living organism, we created a mouse model of human L1 retrotransposition. Here we show that L1 elements can retrotranspose in male germ cells, and that expression of a human L1 element under the control of its endogenous promoter is restricted to testis and ovary. In the mouse line with the highest level of L1 expression, we found two de novo L1 insertions in 135 offspring. Both insertions were structurally indistinguishable from natural endogenous insertions. This suggests that an individual L1 element can have substantial mutagenic potential. In addition to providing a valuable in vivo model of retrotransposition in mammals, these mice are an important step in the development of a new random mutagenesis system.     

SPTLC1 is mutated in hereditary sensory neuropathy, type 1

Hereditary sensory neuropathy type 1 (HSN1, MIM 162400; ref. 1) genetically maps to human chromosome 9q22 (refs. 2-4). We report here that the gene encoding a subunit of serine palmitoyltransferase is located within the HSN1 locus, expressed in dorsal root ganglia (DRG) and mutated in HSN1.     

A 5-bp deletion in ELOVL4 is associated with two related forms of autosomal dominant macular dystrophy

Stargardt-like macular dystrophy (STGD3, MIM 600110) and autosomal dominant macular dystrophy (adMD) are inherited forms of macular degeneration characterized by decreased visual acuity, macular atrophy and extensive fundus flecks. Genetic mapping data suggest that mutations in a single gene may be responsible for both conditions, already known to bear clinical resemblance. Here we limit the minimum genetic region for STGD3 and adMD to a 0.6-cM interval by recombination breakpoint mapping and identify a single 5-bp deletion within the protein-coding region of a new retinal photoreceptor-specific gene, ELOVL4, in all affected members of STGD3 and adMD families. Bioinformatic analysis of ELOVL4 revealed that it has homology to a group of yeast proteins that function in the biosynthesis of very long chain fatty acids. Our results are therefore the first to implicate the biosynthesis of fatty acids in the pathogenesis of inherited macular degeneration.     

Relationship between El Niño /South Oscillation (ENSO) and population outbreaks of some lemmings and voles in Europe

Ecologists have been puzzled by population cycles of lemmings and voles for the over 70 years. At present, our understanding and explanation to this phenomenon remain controversial. Recently, El Niño/South Oscillation has attracted attention of ecologists on its links with population outbreaks of terrestrial animals. This paper aims to investigate the statistical relationship between outbreaks of microtine rodents and ENSO events by scanning available literature. During 1862–1894, outbreaks of Norway lemmings in Norway tended to occur in the Southern Oscillation Index (SOI) peak years or 1 year after the SOI peak years with an approximate significance level (p = 0.057). During 1885–1931, outbreaks of voles in France tended to occur 1 year before the SOI peak years (p = 0.01). During 1946–1993, outbreaks of lemmings and voles in North Finland tended to occur 1 year before the SOI peak years with a significant level (p = 0.022); the peaks of population abundance corresponded well to the SOI trough years (equal to 1 year before the SOI peak years). Outbreaks of common voles in Poland during 1946–1975 tended to occur in the SOI peak years or 1 year before the SOI peak years (p = 0.011), and also tended to occur 1 year before the SOI peak years (p = 0.030). It was also found that the rodent outbreaks in Norway and France, rodent outbreaks in Finland and Poland synchronized well. It was suggested that the ENSO-related climate or food were key factors in causing outbreaks of microtine rodents in Europe.     

关于本性矩阵的注记

该文给出了欧氏空间Rn的子空间的本性矩阵在与子空间的一一对应关系,以及子空间的直和、正交子空间等方面的一些结果.     

Application of support vector machines regression in prediction Shanghai stock composite index

0　IntroductionNeuralnetworks,inrecentyears,havebeensuccessfullyusedformodelingfinancialtimeseries.Researchesrevealneuralnetworkshavebetterresultsindescribingdynamiccharac teristicscomparingwithclassicalstatisticalmethods[1 3] .Back propagation (BP)neuralnetworkisamostpopularlyusedneuralnetworkmodelintheareaoffinancialforecasting .AlthoughBP’sstructureissimple ,ithaspowerfulabilityinsolvingvariousproblems.TherearealsoshortcomingswithBP :needsformanycontrolparameters,difficultyingettingsta…     

Interaction of reelin signaling and Lis1 in brain development

Loss-of-function mutations in RELN (encoding reelin) or PAFAH1B1 (encoding LIS1) cause lissencephaly, a human neuronal migration disorder. In the mouse, homozygous mutations in Reln result in the reeler phenotype, characterized by ataxia and disrupted cortical layers. Pafah1b1(+/-) mice have hippocampal layering defects, whereas homozygous mutants are embryonic lethal. Reln encodes an extracellular protein that regulates layer formation by interacting with VLDLR and ApoER2 (Lrp8) receptors, thereby phosphorylating the Dab1 signaling molecule. Lis1 associates with microtubules and modulates neuronal migration. We investigated interactions between the reelin signaling pathway and Lis1 in brain development. Compound mutant mice with disruptions in the Reln pathway and heterozygous Pafah1b1 mutations had a higher incidence of hydrocephalus and enhanced cortical and hippocampal layering defects. Dab1 and Lis1 bound in a reelin-induced phosphorylation-dependent manner. These data indicate genetic and biochemical interaction between the reelin signaling pathway and Lis1.     

长江中游水灾监测系统的研究和建立

洪涝灾害是长江中游地区最为严重的自然灾害．本文叙述了以遥感和地理信息的应用为基础,将多元资料复合^[1],快速、准确与全天候的对长江中游地区水灾进行监测的方法．介绍了RS和GIS支持下建立起来的监测系统的目标、技术路线、结构流程等,并进行了应用分析．以期利用高新技术手段为长江中游地区有关部门快速了解灾情、指定救灾方案和灾后重建提供决策支持．