Dogs cloned from adult somatic cells

Several mammals--including sheep, mice, cows, goats, pigs, rabbits, cats, a mule, a horse and a litter of three rats--have been cloned by transfer of a nucleus from a somatic cell into an egg cell (oocyte) that has had its nucleus removed. This technology has not so far been successful in dogs because of the difficulty of maturing canine oocytes in vitro. Here we describe the cloning of two Afghan hounds by nuclear transfer from adult skin cells into oocytes that had matured in vivo. Together with detailed sequence information generated by the canine-genome project, the ability to clone dogs by somatic-cell nuclear transfer should help to determine genetic and environmental contributions to the diverse biological and behavioural traits associated with the many different canine breeds.     

Patient Safety Culture,Evidence-Based Practice and Performance in Nursing

Evidence-based practice is a problem-solving approach in which the best available and useful evidence is used by integrating research evidence, clinical expertise, and patient values and preferences to improve health outcomes, service quality, patient safety and clinical effectiveness, and employee performance. This study aimed to identify the effects of nurses’ patient safety culture perceptions and their evidence-based nursing attitudes on the hospital’s patient safety level and employee performance. A cross-sectional design was employed in this study. Participants included nurses working in a state hospital in Burdur, Turkey, and 218 nurses responded to the research instrument (participation rate: 55.7%). Data were collected through face to face interviews that were conducted from February 20–May 20, 2016. The results of the analyses revealed that evidence-based nursing attitudes and hospital safety culture dimensions explained 29.2% of the total variance in the hospital patient safety level, while evidence-based nursing attitudes and hospital safety culture dimensions explained 15.5% of the total variance in nurse performance. The “evidence-based nursing related beliefs and expectations” aspect of the evidence-based nursing attitude dimension, and the “management support for patient safety” aspect of the patient safety culture dimension were significant predictors of nurses’ performance. The present findings add to the increasing interest in improving evidence-based practice and service quality in order to achieve better patient outcomes.     

有限优先下交叉口信号设置的临界流量依据

为完善交叉口信号设置的临界流量依据,引入支路车辆穿越主路车流的风险时间函数和等待时间函数,从微观角度确定了临界间隙与主路流量函数关系,得到了有限优先现象的主路流量最小阈值为500pcu/h,并推导了有限优先下交叉口车均延误模型.在此基础上,从交通安全和效率的角度出发,确定了支路车辆穿越主路车流的最小临界间隙值为4.5s.对比设置信号前后交叉口车均延误的大小,得到了有限优先下1/1,1/2,2/2相交3种常见交叉方式需设置信号的临界流量曲线.与完全优先的主支路交叉口相比,有限优先下3种交叉方式设置信号的临界流量平均增量分别为20.33%,20.95%,21.43%. 研究成果可为信号设置临界流量的确定提供一种新的理论参考.     

非破坏性检测地下LNAPL污染技术研究

采用非破坏性透地雷达,对台湾嘉义市水上乡地下污染情况施测,参考实地钻探报告,对污染范围进行描绘,经比对施测结果与钻探报告,透地雷达检测比水轻非水相液体(LNAPL)污染结果准确,污染范围判定吻合.     

对权利观念的兴起与自然法观念的嬗变的简要考察

在西方政治文化中，原生型的自然法是对涵蕴于自然之中的客观秩序的描述。随着权利观念在12世纪的兴起，人们的自然法概念开始发生改变，权利代替义务逐渐成为自然法的基本内涵。     

Programmable nanowire circuits for nanoprocessors

A nanoprocessor constructed from intrinsically nanometre-scale building blocks is an essential component for controlling memory, nanosensors and other functions proposed for nanosystems assembled from the bottom up. Important steps towards this goal over the past fifteen years include the realization of simple logic gates with individually assembled semiconductor nanowires and carbon nanotubes, but with only 16 devices or fewer and a single function for each circuit. Recently, logic circuits also have been demonstrated that use two or three elements of a one-dimensional memristor array, although such passive devices without gain are difficult to cascade. These circuits fall short of the requirements for a scalable, multifunctional nanoprocessor owing to challenges in materials, assembly and architecture on the nanoscale. Here we describe the design, fabrication and use of programmable and scalable logic tiles for nanoprocessors that surmount these hurdles. The tiles were built from programmable, non-volatile nanowire transistor arrays. Ge/Si core/shell nanowires coupled to designed dielectric shells yielded single-nanowire, non-volatile field-effect transistors (FETs) with uniform, programmable threshold voltages and the capability to drive cascaded elements. We developed an architecture to integrate the programmable nanowire FETs and define a logic tile consisting of two interconnected arrays with 496 functional configurable FET nodes in an area of ～960 μm(2). The logic tile was programmed and operated first as a full adder with a maximal voltage gain of ten and input-output voltage matching. Then we showed that the same logic tile can be reprogrammed and used to demonstrate full-subtractor, multiplexer, demultiplexer and clocked D-latch functions. These results represent a significant advance in the complexity and functionality of nanoelectronic circuits built from the bottom up with a tiled architecture that could be cascaded to realize fully integrated nanoprocessors with computing, memory and addressing capabilities.     

Directed self-assembly of a colloidal kagome lattice

A challenging goal in materials chemistry and physics is spontaneously to form intended superstructures from designed building blocks. In fields such as crystal engineering and the design of porous materials, this typically involves building blocks of organic molecules, sometimes operating together with metallic ions or clusters. The translation of such ideas to nanoparticles and colloidal-sized building blocks would potentially open doors to new materials and new properties, but the pathways to achieve this goal are still undetermined. Here we show how colloidal spheres can be induced to self-assemble into a complex predetermined colloidal crystal-in this case a colloidal kagome lattice-through decoration of their surfaces with a simple pattern of hydrophobic domains. The building blocks are simple micrometre-sized spheres with interactions (electrostatic repulsion in the middle, hydrophobic attraction at the poles, which we call 'triblock Janus') that are also simple, but the self-assembly of the spheres into an open kagome structure contrasts with previously known close-packed periodic arrangements of spheres. This open network is of interest for several theoretical reasons. With a view to possible enhanced functionality, the resulting lattice structure possesses two families of pores, one that is hydrophobic on the rims of the pores and another that is hydrophilic. This strategy of 'convergent' self-assembly from easily fabricated colloidal building blocks encodes the target supracolloidal architecture, not in localized attractive spots but instead in large redundantly attractive regions, and can be extended to form other supracolloidal networks.     

Addition of cerium and yttrium to ferritic steel weld metal to improve hydrogen trapping efficiency

The applicability of Ce and Y as promising candidate elements to form irreversible traps in weld metal was investigated by thermal desorption spectroscopy (TDS) with gas chromatography (GC).The precise nature of the precipitate particles newly formed in the weld metal by the addition of Ce and Y to a certain alloy system was characterized.Moreover,the hydrogen trapping efficiency expressed as the reduction of the diffusible hydrogen in the weld metal was analyzed.The results showed that the addition of Ce and/or Y to this alloy system led to the formation of a mixed type of (Ce,Ti)-based oxide,(Y,Ni)-based carbide,or (Ce,Y,Ti)-based oxide particles.Because of the high activation energy of the mixed type of particles (≥ 150 kJ/mol),the trapping efficiency for hydrogen was considered to be sufficiently high to effectively reduce the diffusible hydrogen content.     

Rational design of carbon shell-encapsulated cobalt nanospheres to enhance microwave absorption performance

Co@C core–shell nanospheres highly dispersed on carbon supports were rationally designed to improve the microwave absorbing property of the composite material, and fabricated by one pot thermal decomposition and simple annealing process. The Co nanospheres were completely encapsulated with thin carbon shells, which can effectively prevent the oxidation of the Co surface. Additionally, the particle size of Co nanospheres were properly controlled to optimize the electromagnetic property of the composite material. As a result, the lightweight Co@C/C composites with the particle size of 20 nm exhibited much enhanced microwave absorption properties. The improved microwave absorption performance is attributed mainly to the enhanced isotropic ratio and impedance matching of magnetic composites via tuning the Co particle size. Therefore, the welldesigned core-shell Co@C composite structure will provide a new insight for the development of high performance microwave absorbers.