Serotonin-stimulated protein phosphorylation in aortic smooth muscle cells

Summary The effects of serotonin on the formation of inositol phosphates and protein phosphorylation were examined in cultured smooth muscle cells. Serotonin stimulated the formation of [³H]inositol monophosphate, [³H]inositol bisphosphate and [³H]inositol trisphosphate. This effect was prevented by 5-HT₂ specific antagonist, 6-methyl-1-(1-methylethyl)ergoline-8-carboxylic acid, 2-hydroxy-1-methylpropyl ester [Z]-2-butenedioate (LY53857). Serotonin stimulated the phosphorylation of many polypeptides, among which a 20 kDa polypeptide was the most prominent. The phosphorylation was also inhibited by LY53857. LY53857 alone produced no effects on protein phosphorylation. The 20 kDa polypeptides were also phosphorylated by the addition of 12-O-tetradecanoylphorbol-13-acetate. These results suggest that serotonin stimulates protein phosphorylation through 5-HT₂ receptors and possibly activates protein kinase C in intact vascular smooth muscle cells.Part of the data contained in this paper was presented at the 74th local meeting of the Japanese Society of Pharmacology at Kanagawa.     

The effect of a low protein diet on the adipocytes of rat perigenital fat

Weanling rats were fed a low protein diet for 6 weeks and their weights were 50% less than controls. There were significantly fewer adipocytes per microgram adipose tissue, but estimates of the number of adipocytes per rat indicated that the diet had much less effect on adipocyte number than on b. wt.     

The heating of gas in a galaxy cluster by X-ray cavities and large-scale shock fronts

Most of the baryons in galaxy clusters reside between the galaxies in a hot, tenuous gas. The densest gas in their centres should cool and accrete onto giant central galaxies at rates of 10-1,000 solar masses per year. No viable repository for this gas, such as clouds or new stars, has been found. New X-ray observations, however, have revealed far less cooling below X-ray temperatures than expected, altering the previously accepted picture of cooling flows. As a result, most of the gas must be heated to and maintained at temperatures above approximately 2 keV (ref. 3). The most promising heating mechanism is powerful radio jets emanating from supermassive black holes in the central galaxies of clusters. Here we report the discovery of giant cavities and shock fronts in a distant (z = 0.22) cluster caused by an interaction between a radio source and the hot gas surrounding it. The energy involved is approximately 6 x 10(61) erg, the most powerful radio outburst known. This is enough energy to quench a cooling flow for several Gyr, and to provide approximately 1/3 keV per particle of heat to the surrounding cluster.     

IFITM3 restricts the morbidity and mortality associated with influenza

The 2009 H1N1 influenza pandemic showed the speed with which a novel respiratory virus can spread and the ability of a generally mild infection to induce severe morbidity and mortality in a subset of the population. Recent in vitro studies show that the interferon-inducible transmembrane (IFITM) protein family members potently restrict the replication of multiple pathogenic viruses. Both the magnitude and breadth of the IFITM proteins' in vitro effects suggest that they are critical for intrinsic resistance to such viruses, including influenza viruses. Using a knockout mouse model, we now test this hypothesis directly and find that IFITM3 is essential for defending the host against influenza A virus in vivo. Mice lacking Ifitm3 display fulminant viral pneumonia when challenged with a normally low-pathogenicity influenza virus, mirroring the destruction inflicted by the highly pathogenic 1918 'Spanish' influenza. Similar increased viral replication is seen in vitro, with protection rescued by the re-introduction of Ifitm3. To test the role of IFITM3 in human influenza virus infection, we assessed the IFITM3 alleles of individuals hospitalized with seasonal or pandemic influenza H1N1/09 viruses. We find that a statistically significant number of hospitalized subjects show enrichment for a minor IFITM3 allele (SNP rs12252-C) that alters a splice acceptor site, and functional assays show the minor CC genotype IFITM3 has reduced influenza virus restriction in vitro. Together these data reveal that the action of a single intrinsic immune effector, IFITM3, profoundly alters the course of influenza virus infection in mouse and humans.     

Relation of ATPase activities to iron uptake in rabbit and cat erythroid cells

Zusammenfassung Fe⁵⁹-Aufnahme in Kaninchen-Retikulozyten ist durch erhöhte Werte des Isotops in der wasserlöslichen Fraktion und erniedrigten Werten im Stroma der Katzen-Retikulozyten charakterisiert. Bei Kaninchen- und Katzen-Erythrozyten ist dieses Verhältnis umgekehrt. Na⁺ und K⁺ aktivierte ATPase zeigt die höchsten Werte in den Kaninchen-Retikulozyten.

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Supported by National Science Foundation Grant No. GB-2295 and Public Health Service Grant No. AM 09069 and by a NIH Predoetoral Fellowship, No. 5-Fl-GM-29843 toW. C. Wise.