Serotyping Plasmodium falciparum malaria with S-antigens

The heat-stable proteins known as S-antigens which are associated with Plasmodium falciparum malarial infections in man show considerable serological diversity. Usually, different S-antigens appear in separate malarial episodes in individuals exposed to reinfection. The antigens have a worldwide distribution in endemic areas but the restricted number and characteristic specificity of S-antigens recovered from experimentally infected Aotus monkeys suggest that these antigens might provide suitable markers for serotyping isolates of P. falciparum. Two kinds of evidence to support this idea are presented here. First, S-antigens which characterise an isolate of parasites are shown to retain their specificity over long periods of passage either in vivo or in vitro. Second, predictable mixtures of S-antigens can be recovered from cultures of P. falciparum after deliberately mixing isolates which each give rise to characteristic S-antigens.     

Local marriage markets in Great Britain: how diverse?

Estimates are made of the number of potential marriage partners available for unmarried men and women, by age, in Great Britain in 1991 and how this varies across local districts. The preferences of men and women in relation to partner ages are taken into account in the estimates. Average partner supply declines by age for women and increases with age for men. Marriage markets differ between local areas but the differentiation is not as substantial as in many other aspects of local demography and is a good deal less than the variation that occurs through time. Young women and older men have advantageous marriage markets almost everywhere while young men and older women are at a disadvantage in almost all areas.     

Synaptic defects in ataxia mice result from a mutation in Usp14, encoding a ubiquitin-specific protease

Mice that are homozygous with respect to a mutation (ax(J)) in the ataxia (ax) gene develop severe tremors by 2-3 weeks of age followed by hindlimb paralysis and death by 6-10 weeks of age. Here we show that ax encodes ubiquitin-specific protease 14 (Usp14). Ubiquitin proteases are a large family of cysteine proteases that specifically cleave ubiquitin conjugates. Although Usp14 can cleave a ubiquitin-tagged protein in vitro, it is unable to process polyubiquitin, which is believed to be associated with the protein aggregates seen in Parkinson disease, spinocerebellar ataxia type 1 (SCA1; ref. 4) and gracile axonal dystrophy (GAD). The physiological substrate of Usp14 may therefore contain a mono-ubiquitin side chain, the removal of which would regulate processes such as protein localization and protein activity. Expression of Usp14 is significantly altered in ax(J)/ax(J) mice as a result of the insertion of an intracisternal-A particle (IAP) into intron 5 of Usp14. In contrast to other neurodegenerative disorders such as Parkinson disease and SCA1 in humans and GAD in mice, neither ubiquitin-positive protein aggregates nor neuronal cell loss is detectable in the central nervous system (CNS) of ax(J) mice. Instead, ax(J) mice have defects in synaptic transmission in both the central and peripheral nervous systems. These results suggest that ubiquitin proteases are important in regulating synaptic activity in mammals.     

Beaver herbivory of willow under two flow regimes: a comparative study on the Green and Yampa rivers

The effect of flow regulation on plant-herbivore ecology has received very little attention, despite the fact that flow regulation can alter both plant and animal abundance and environmental factors that mediate interactions between them. To determine how regulated flows have impacted beaver ( Castor Canadensis ) and sandbar willow ( Salix exigua ) ecology, we first quantified the abundance and mapped the spatial distribution of sandbar willow on alluvial sections of the flow-regulated Green River and free-flowing Yampa River in northwestern Colorado. We then established 16 and 15 plots (1 m x 2.7 m) in patches of willow on the Green and Yampa Rivers, respectively, to determine whether rates of beaver herbivory of willow differed between rivers (Green versus Yampa River), seasons (fall-winter versus spring-summer), and years (spring 1998-spring 1999 versus spring 1999-spring 2000). Areal extent of willow was similar on each river, but Green River willow patches were smaller and more numerous. Beavers cut more stems during fall and winter than spring and summer and cut over 6 times more stems (percentage basis) on the Green River than on the Yampa River. We attribute the between-river difference in herbivory to higher availability of willow, greater beaver density, and lower availability of young Fremont cottonwood ( Populus deltoids subsp. wislizenii ; an alternative food source) on the Green River. Flow regulation increased willow availability to beaver by promoting the formation of island patches that are continuously adjacent to water and feature a perimeter with a relatively high proportion of willow interfacing with water.     

Quantum annealing with manufactured spins

Many interesting but practically intractable problems can be reduced to that of finding the ground state of a system of interacting spins; however, finding such a ground state remains computationally difficult. It is believed that the ground state of some naturally occurring spin systems can be effectively attained through a process called quantum annealing. If it could be harnessed, quantum annealing might improve on known methods for solving certain types of problem. However, physical investigation of quantum annealing has been largely confined to microscopic spins in condensed-matter systems. Here we use quantum annealing to find the ground state of an artificial Ising spin system comprising an array of eight superconducting flux quantum bits with programmable spin-spin couplings. We observe a clear signature of quantum annealing, distinguishable from classical thermal annealing through the temperature dependence of the time at which the system dynamics freezes. Our implementation can be configured in situ to realize a wide variety of different spin networks, each of which can be monitored as it moves towards a low-energy configuration. This programmable artificial spin network bridges the gap between the theoretical study of ideal isolated spin networks and the experimental investigation of bulk magnetic samples. Moreover, with an increased number of spins, such a system may provide a practical physical means to implement a quantum algorithm, possibly allowing more-effective approaches to solving certain classes of hard combinatorial optimization problems.