Neuroglobin mutation associated with hypoxia adaptation in Tibet chicken

Globin protein family plays an important role in storing and transporting oxygen. As a newly reported globin, the revealed function of neuroglobin includes binding and storing oxygen as well as facilitating the utilization of oxygen in neuronal cells. In the present study, mutations in exons of chicken Ngb gene were identified with the method of sequencing and created restriction site PCR in Tibet chicken and other four lowland chicken breeds. The mutations of Lys-2224(E4)-Asn and Ser-2279(E4)-Gly were identified in exon 4 of the gene. The Lys-2224(E4)-Asn mutation existed only in Tibet chicken and the mutation frequencies increased with increasing altitude. Analysis of the haplotype and diplotype of the two mutations in Tibet chicken populations of different altitudes showed that the frequencies of TG haplotype and TTGG diplotype also increased with increasing altitude, while the reverse tendency was found on GGAA diplotype. Under the hypoxic simulation incubation, the main haplotype was TG in living embryos and GA in dead embryos. The results showed that the Lys-2224(E4)-Asn mutation may be a specific mutation associated with hypoxia adaptation in Tibet chicken.     

Neuroglobin mutation associated with hypoxia adaptation in Tibet chicken

Globin protein family plays an important role in storing and transporting oxygen. As a newly reported globin, the revealed function of neuroglobin includes binding and storing oxygen as well as facilitating the utilization of oxygen in neuronal cells. In the present study, mutations in exons of chicken Ngb gene were identified with the method of sequencing and created restriction site PCR in Tibet chicken and other four lowland chicken breeds. The mutations of Lys-2224(E4)-Asn and Ser-2279(E4)-Gly were identified in exon 4 of the gene. The Lys-2224(E4)-Asn mutation existed only in Tibet chicken and the mutation frequencies increased with increasing altitude. Analysis of the haplotype and diplotype of the two mutations in Tibet chicken populations of different altitudes showed that the frequencies of TG haplotype and TTGG diplotype also increased with increasing altitude, while the reverse tendency was found on GGAA diplotype. Under the hypoxic simulation incubation, the main haplotype was TG in living embryos and GA in dead embryos. The results showed that the Lys-2224(E4)-Asn mutation may be a specific mutation associated with hypoxia adaptation in Tibet chicken.     

加碳方式对磷酸铁锂动力学及电化学性能的影响

以FeSO4.7H2O和NH4H2PO4为原料,以H2O2为氧化剂,通过液相沉淀法制得前驱体FePO4,然后通过碳热还原反应制得LiFePO4。采用2种加碳方式:方法一是先制得FePO4,然后加入乙炔黑混合后高温合成LiFePO4;方法二是先把乙炔黑分散在液相中,然后通过液相沉淀制得含碳的FePO4,再高温合成LiFePO4。研究结果表明:采用方法二合成的LiFePO4动力学及电化学性能比方法一合成的优。采用方法二所制备的LiFePO4样品以0.1C的倍率放电,其比容量达149 mA.h/g;当放电倍率为1C时,其比容量为124 mA.h/g,且循环性能良好。     

浅谈现代钢结构建筑

经济的飞速发展,使得钢结构技术越来越多地应用在各种民用建筑和工业建筑中。更多地了解钢结构的技术,避免实际钢结构建筑中经常出现的问题,这样现代钢结构建筑才能越做越好。     

道路交通事故黑点鉴别及改善对策研究综述

本文介绍了道路交通事故黑点的定义和黑点鉴别的方法,提出了改善事故黑点的对策.     

一类病结构系统的多指标模糊评价方法

针对一类病结构系统的多指标评价问题进行了讨论．阐述了以评议尺度分析为核心进行模糊综合评价的思想，给出了建立尺度标准化矩阵、评议信息的区间数表示和构造综合评价矩阵的方法．     

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应用蒙特卡洛法对吉林省境内的３种不同类型的小流域的洪水三要素进行了模拟，得出洪水三要素的理论值。     

高速公路混凝土桥面铺装的病害成因及处治措施

随着高速公路的快速发展，高速公路桥梁结构型式得到了不断的创新，桥梁跨度也越来越大，但就目前安徽省高速公路桥面铺装的情况来看，设计与施工大多沿用传统的习惯做法：一般自上而下采用沥青混凝土面层＋防水涂料＋防水混凝土模式，已不能满足重载、重交通的发展要求，使得通车不久后的桥面铺装层逐渐产生起皮、泛砂、剥落、露骨、空鼓、开裂、松散和破碎等现象，严重影响了行车安全和舒适性以及桥梁的使用寿命。该文就高速公路混凝土桥面铺装的病害现状、成因进行简要分析，并对处治措施进行探讨，为设计、施工提供参考。     

ADSL宽带接入技术的研究与应用

简单介绍了各种宽带接入技术,重点介绍了ADSL(非对称数字用户线环路)接入技术及其应用,并给出详细的系统设计方案.     

寄生于蝗虫六索线虫属一新种

在厦门岛东南部花生地土壤及疣蝗（Trilophidia anmulata)体内采集到一种六索线虫，研究发现与其它种有较大差异，命名为厦门六索线虫新虫Hexamamis Xiamenensis sp.nov，并指出它的鉴别特征。