二次游离氧化钙测定条件

研究了测定熟料中二次游离氧化钙的条件。结果表明 :只要增大分析试样的细度 ,保证足够长的萃取时间 ,用甘油 乙醇法和乙二醇 乙醇法都能测定出熟料中的二次f CaO ,乙二醇 乙醇比甘油 乙醇萃取速度快。     

水稻籼粳杂种多倍体的研究

以秋水仙碱诱导的水稻籼粳杂多倍体植株中，部分植株的体细胞呈现具有整倍体和非整倍体染色体数细胞嵌合现象，其中四倍体细胞最高可占35．3％，其余均为染色体数低于48条的各种细胞，与二倍体植株相比，这些植株的叶表皮气孔增大，气孔数减少，花粉粒直径增大都是十分显的，与多倍体植株无明显区别，并对这类植株根尖体细胞中存在具各种染色体数目细胞嵌合现象可能的原因进行了分析。     

Microelement analysis of source of raw materials of terracotta warriors and horses of Qin’s Mausoleum in pits No. 1 and No. 2

In order to search for sources of the raw material of the Qin’s terracotta warriors and horses, we selected 20 samples of pottery warriors and pottery horses of the Qin’s Mausoleum from pit No. 1, 19 samples of pottery warriors and brick from pit No. 2, 20 samples of clays near the Qin’s Mausoleum and 2 samples of Yaozhou porcelain bodies. The 32 kinds of element content of each sample were measured by neutron activation analysis (NAA). These data were analyzed by fuzzy cluster analysis, the trend cluster analysis diagram was obtained. The results showed that samples of terracotta warriors and horses from pits No.1 and No. 2 were closely related to the loam layer near Qin’s Mausoleum, but their relations with the loess layers were not so close, and they were out of all relation to Yaozhou porcelain bodies. Thus, it could be deduced that the Li Mountain clay may be considered as the original place of the raw materials of the terracotta warriors and horses, the kiln sites might be located nearby the Qin’s Mausoleum.     

Modeling studies on the changes of the Subtropical High over the Western Pacific during the last 100 years

Intensity index series of Subtropical High over the Western Pacific was constructed according to modeling results by using of CCM3.6 atmospheric model with climatologically SST forcing for 30 years, and with observed SST forcing from 1900 to 2000. The index series constructed by modeling closely correlated to the observations for 1951-1999 (c.c. 0.72) and to the statistically reconstructed one for 1900-1950 (c.c. 0.51). The index series for 1900-1999 showed significant 80 a and 40 a periodicity. A minimum of persistence in seasonal variations of the index occurred in Autumn, which was called "Autumn Barrier". It may relate to the "Spring Barrier", found in the seasonal variations of SST over the Eastern Equatorial Pacific. The increasing trend shown in the index series for the 20th century may associate with the global warming caused by enhancement of greenhouse effect.     

Construction of multivalent DNA vaccines forMycobacterium tuberculosis and its immunogenicity

The coding regions of Ag85B MPT-64, and ESAT-6 secreted proteins were cloned initially into the eukaryotic expression vector pJW4303, then transformed to E. coli Top 10 strain for plasmid DNA extraction and further analysis. Plasmids containing the right insertion were sequenced to confirm their identity. COS7 cells were transfected with a mixture containing serially diluted plasmid DNA encoding three secreted proteins and Lipofectin (Gibco). The supernatants and pellets prepared from various cell lines were run on SDS-PAGE gel and the expression of these proteins in COS7 cells were demonstrated by immunoblot using polyclonal or monoclonal antiserum of M.TBH37Rv. 21 days after first vaccination of C57BL-6 mice by all three recombinant eukaryotic expressing vectors, antibody titer for Ag85B reached 1∶3200. 21 days after second vaccination, the antibody titer reached 1∶102400. The highest antibody levels induced by multivalent vaccines after the second injection were equal to or even greater than the highest antibody levels of single DNA vaccine reported in literature after third injections. Antibody titer of MPT-64 was 1∶50 after the first injection and it reached 1∶200 after the second injection. No antigen-specific antibody against ESAT-6 was detected in sera harvested from immunized mice 21 days after both injections. Antigen-specific IFN-g level of Ag85B was 110 pg/mL while no antigen-specific IFN- g level of ESAT-6 and MPT-64 was detected even after third injections. To our knowledge, it is the first time that studies of polyvalent recombinant DNA vaccines against TB were carried out in C57BL-6 mice. Our results indicated that multiple DNA vaccines could be used to enhance protective responses against M.TB.     

脉冲电晕技术降低柴油机微粒排放的实验研究

利用线－筒式荷电装置和高压窄脉冲电源来降低柴油机的微粒排放，分析了不同电源电压和脉冲频率下排气微粒的脱除效率，并在不同转速和负荷下进行了实验研究。研究结果表明，电源电压和脉冲频率是影响微粒脱除效率的两个主要因素，其中电源电压是最主要的影响因素，利用脉冲电晕放电降低柴油机的微粒排放效率明显的，是一种很有发展前途的控制尾气排放的新技术。     

偏最小二乘--荧光光度法同时测定多种酚体系

酚类化合物具有平面共轭的分子结构,因而具有较强的内源荧光。在苯酚含量为0～1×10~(-4)mol·L~(-1),对甲基酚含量在0～1×10~(-4)mol·L~(-1),邻苯二酚含量在0～5×10~(-5)mol·L~(-1)时线性关系较好.但是在线性范围内3种酚的荧光强度并不具有良好的线性加和性,而且苯酚、对甲酚、邻苯二酚的荧光光谱严重重叠,常规分析方法难以实现多组分同时测定。文中采用了偏最小二乘法(PLS)处理该3组分的荧光光谱数据,实现了对模拟样品中3组分的同时测定。     

长江上游生态修复与重点区域治理研究

在分析水土流失对洪水、河道影响与洪灾形成的基础上，通过比较分析与实地考察，阐述了四川省域水土保持在长江上游生态建设中的地位。指出了长江上生态修复的重点区域，并就治理对策作了分析探讨。     

玻璃纤维增强塑料加固砼梁的抗剪试验

通过对15根玻璃增强塑料（GFRP）片材加固混凝土梁的抗剪试验，研究GFRP片材的形状、加固方式、加固率、以及梁的剪跨比对加固梁抗剪承载力和破坏模式的影响。文中采用拱形桁架模型解释GFRP加固梁的剪切破坏机理，提出GFRP片材加固钢筋混凝土梁的抗剪承载力的计算公式。     

扫描隧道显微镜畸变图象的校正

从扫描隧道显微镜（STM）的工作原理出发，分析了STM图象畸变产生的原因，建立了一种相应的物理数学模型，利用计算机通过数值解，校正了STM畸变图象，并得到了满意的效果。