Distribution of ghrelin-like immunoreactive cells in amphioxus, Branchiostoma belcheri – A study of immunohistochemistry

The distribution of ghrelin-like immunoreactive cells in amphioxus (Branchiostoma belcheri) was investigated by using immunohistochemical staining with rabbit antiserum against synthetical mammalian ghrelin. The results showed that ghrelin-like immunoreactive cells were distributed widely in the nervous system, Hatschek’s pit, wheel organ, digestive tract and gonads (ovary and testis). In nervous system, ghrelin-like immunoreactive neurons and their protrusions were distributed speci?cally on the dorsal side, ventral side and funnel part of brain vesicle, with a few dispersive immunoreactive nerve cells and their ?bers in nerve tube. Ghrelin-like immunoreactivities were also detected in Hatschek’s pit epithelial cells and wheel organ cells, with positive substance located along cell membrane. In digestive tract, ghrelin-like immunoreactive cells existed in hepatic diverticulum, anterior and posterior region of midgut, and could be classified into two types, closed- and opened-type endocrine cells. The number of positive cells was most in hepatic diverticulum, secondary in posterior region of midgut and least in anterior region of midgut. In gonads, ghrelin-like immunoreactive substance was detected in oogonia, oocytes and follicle cells in ovary at the small and large growth stages and in early spermatogenic cells and Sertoli cells in testis. The extensive distribution of ghrelin-like cells in amphioxus suggested that these kinds of cells are conservative in evolution and diversified in function. At the same time, we found for the ?rst time that ghrelin-like immunoreactive cells existed in brain vesicle and Hatschek’s pit, which provided new morphological evidence for the existence of an activation pathway between brain vesicle and Hatschek’s pit for the regulation of growth hormone excretion.     

基于车灯的无线反馈非接触供电电路设计

为了能在汽车的引擎盖等活动部位装设车灯,给出了一种无线反馈稳压的非接触供电车灯电路.无线反馈稳压电路通过无线反馈控制电路的输出电压,达到稳定负载功率的目的,实验证明电路可行.     

汽车减震器的建模与模态分析

运用Solid Works对单筒减震器进行三维建模,并导入到ANSYS Workbench软件对减震器进行模态分析,获取了其前6阶固有频率和振型。分析了各阶振动模态的特点,得到变形最大区域、变形量情况及振动响应的表现形态。该结果有利于汽车减震器的动态特性分析和整体设计。     

聚酰亚胺/SiO2-Al2O3杂化薄膜亚胺化反应分析及分子结构预测

本文采用溶胶-凝胶法制备聚酰亚胺/二氧化硅-氧化铝（PI/SiO2-Al2O3）杂化薄膜。运用红外光谱仪、示差扫描量热仪和紫外-可见分光光度计分别测试了亚胺化过程中各阶段杂化膜的特征基团吸收峰的变化,反应热以及紫外-可见光透过率。使用原子力显微镜分析了无机相在亚胺化各阶段的形貌变化。通过这些分析推测了杂化薄膜的分子结构。     

2-D viscous magnetohydrodynamics simulation of plasma armatures with the CE/SE method

A possible two-dimensional viscous magnetohydrodynamics (MHD) model is applied to investigating the plasma armature in a railgun. The space-time conservation element and solution element (CE/SE) method for solving the coupled Navier-Stokes equations and Maxwell equations was devised. The distributions of physical parameters of the plasma may thus be evaluated. The results show that extremely high pressure can always be observed ahead of the projectile, and the Lorentz force is the main propulsion. The distribution of temperature is in a good agreement with the results predicted by the law of radiation at the boundaries. Due to convection, the circulation patterns of velocity are evident in both the cases considering inviscid and viscous effect. Furthermore, the velocity and acceleration oscillate over time until a new steady state is achieved. This model efficiently captures the salient features of the physical phenomena, and contributes to further studies of MHD problems in plasma armature. Supported by the National Natural Science Foundation of China (Grant No. 10672080)     

可实测FRP-混凝土最大粘结滑移量的双拉试件

探讨既有试件难以测出纤维复合材料(FRP)一混凝十最大粘结滑移量δu的原因,进而提出一种可稳妥实测δu的概念舣拉试件.并经实验研究、设计定型.结果表明,双拉试件的FRP-凝土界面局部范围内有较大滑移时,也不会发生界面整体剥离,从而克服了既有试件的缺点.其后又制作10个试件,探讨δu的确定方法和范围.结果显示,在本文实验条件下,实测δu为0.31～O.52 mm左右.     

Effect of Mn cluster on the formation of superoxide radicals in photoinhibition of photosystem Ⅱ

To further realize the action of superoxide radicals (O₂^{− .}) in photoinhibition of photosystem Ⅱ (PSⅡ), we employed 5,5-dimethyl-1-pyrroline-N-oxide (DMPO) as a spin trap, associated with EPR spectroscopy, to study the effect of illumination time on O₂^{− .} formation during high light photoinhibition in PSⅡ membranes and Mn-depleted PSⅡ membranes. Results indicated that the removal of Mn cluster from PS
membranes has a strong influence on the dynamics of superoxide formation. The relative mechanism was also discussed. These novel findings may further promote the studies of the structure and function of PSⅡ and the mechanism of photoinhibition.     

介质阻挡放电照相技术的应用

在研究介质阻挡放电照相机理和质量的基础上,采用高压纳秒脉冲介质阻挡放电技术,以硬币、植物叶片以及人体手指为样品,对五种应用实例进行了实验尝试和讨论。结果表明,用该方法在拍摄单一色彩的非平面图案时,所得的图像比用普通光学摄影获得的图像更为完整、清晰、且对比度高。该技术可应用于非平面样品图案图像的提取和复印、植物新鲜程度的鉴别、人体指纹的获取以及特殊摄影等领域。     

二(2-乙基己基)磷酸铵反胶束萃取氨基酸特性

以表面活性剂二（２－乙基己基）磷酸铵作为反胶束,测定其浓度对萃取精氨酸的影响,确定最适宜的表面活性剂浓度。测定水相中ＮａＣｌ浓度对反胶束吸水率和萃取精氨酸的影响,结果表明,以二（２－乙基己基）磷酸锭为表面活性剂所形成的反胶束具有较其它反胶束更强的萃取能力,具有良好的吸水性能,适合于从高盐浓度（４．５ｍｏｌ．Ｌ＾－１）的水溶液中,萃取出氨基酸。     

Cu2+配位桥联作用组建荧光性多层膜

借助Au—S化学键的作用,在金基底上组装DL-半胱氨酸(Cys),形成单层自组装膜Cys／Au．然后,利用Cu^2 的配位桥联作用,将卜萘胺乙酸(NAA)组装于Cys／Au上,构建一种新型的自组装多层膜NAA／Cu／Cys／Au．文中采用电化学、荧光光谱、电子能谱等方法表征自组装膜的结构,采用电化学阻抗谱技术和循环伏安法研究该荧光性自组装膜的性能．结果表明,基于铜离子配位作用的NAA／Cu／Cys／Au膜与基于静电作用制得的双层膜NAA／Cys／Au相比,具有更好的导电性。