Caspase-3抑制剂的筛选体系构建及药物筛选

Caspase-3是细胞凋亡途径中重要的靶蛋白,研究Caspase-3抑制剂或激活剂,对治疗心脑血管疾病、肿瘤等具有积极意义.利用原核表达系统高效表达Caspase-3,并以表达的粗酶构建基于Caspase-3抑制剂的体外高通量筛选模型,随后对实验室保存的960种放线菌次级代谢产物进行筛选,获得9株具备分泌Caspase-3抑制剂活性的菌株,随后进一步复筛,确认了2株具备分泌高活性的Caspase-3抑制剂的菌株pwh890和pwh891,均可抑制80%的Caspase-3活性.最后对pwh890分泌的次级代谢产物进行初步分离纯化,获得31.3mg纯度较高的抑制剂,为后续的抑制剂的结构解析和改造奠定基础.     

沥青混合料蠕变试验数据处理与粘弹性计算

为了获得沥青混合料粘弹性本构关系,并利用这种本构关系进行各种数值计算,结合贯入试验中采集到的蠕变数据,采用Matlab软件对蠕变柔量进行拟合,得到了由广义Maxwell模型和Burgers模型表示的粘弹性参数;针对ANSYS有限元软件的计算要求,推导了将其转化为Prony级数形式的计算公式.通过实例计算表明:四参数Burgers模型和六参数Maxwell模型的拟合相对误差分别小于0.7%和1.3%,利用Prony级数方法得到的计算结果与理论解误差不大于0.001%.Burgers模型比广义Maxwell模型更能准确表达沥青混合料的本构关系;Prony级数的转化公式方法简单,计算精确.     

洞室群围岩应力集中效应放大现象的数值模拟

为了研究洞室群开挖过程中围岩应力集中效应放大现象的变化规律,本文根据不同的截面形状、不同的水平距离、不同的空间位置,利用3D-Sigma软件设计了4组洞室群模型,第1组包含5个模型,其他组均包含3个模型,以此来模拟后期开挖洞室的不同布置方案对前期洞室的影响规律.通过在拱顶、拱底和左右拱脚等部位设立应力监测点,得到了后期洞室群开挖过程中前期洞室围岩中代表性部位应力值的变化.最后从最大主应力和最小主应力两个方面,详细分析了开挖过程中随着洞室间距以及后期开挖洞室位置的变化洞室群围岩应力集中情况,得出了洞室群开挖过程中围岩应力集中效应的放大规律.该规律对深部洞室群稳定性控制具有指导意义.     

偏心受压构件可靠度分析的极限状态方程

分析了偏心受压构件可靠度计算采用的极限状态方程。对钢筋混凝土大小偏心受 压和不允许开裂的混凝土受压构件作了以轴向压力表达和以弯矩表达的极限状态方程 的可靠度对比计算。计算结果表明，以通常采用的轴向压力表达的极限状态方程进行可靠度分析．不能确切地反映荷载变异性对可靠度的影响．因此所计算的可靠指标也 是不确切的．建议偏心受压构件可靠度分析采用以弯矩表达的极限状态方程。     

Cupric ion release and cytotoxicity for Yuangong Cu-IUDs and the release behavior of indomethacin for medicated 220 Cu-IUD

These years Yuangong copper-bearing intrauterine devices （Cu-IUDs） have been used because of less side effects in use. The corrosion of copper is essential to the success of contraception, and the release behavior of indomethacin from medicated Cu-IUD is related to its therapeutic effect. In this study, analytical methods were established to investigate the release behavior of cupric ion of three kinds of Yuangong Cu-IUDs and indomethacin of medicated Yuangong 220 Cu-IUD. Cu-IUDs were incubated in simulated uterine solution （SUS）. The concentrations of cupric ion and indomethacin were analyzed by flame atomic absorption spectrometer （FAAS） for 60 days and UV/vis -3310 spectrophotometer for 60 days, respectively. The morphology of copper after corrosion was characterized by SEM. In addition, we detected cytotoxicity by MTT of L929 mouse fibroblasts cells caused by extracts of the three Yuangong Cu-IUDs. The release behavior of cupric ion for three kinds of Yuangong Cu-IUDs was biphasic, which consisted of the initial burst release and then slow and constant release. In vitro release experiment confirmed a biphasic release of indomethacin from Yuangong 220. The copper wire of Yuangong Cu-IUDs showed uneven corrosion. The RGR value of Yuangong 365 Cu-IUD was smaller than that of medicated Yuangong 220 Cu-IUD and RGR value of medicated Yuangong 220 Cu-IUD was smaller than that of Yuangong 300 Cu-IUD. The cupric ion release and indomethacin release showed biphasic. Indomethacin increased the cupric ion release rate and might diminish the adverse effects caused by burst release of cupric ion. The toxicity grade of these three Yuangong Cu-IUDs was 4. We should canvass the adverse events of Cu-lUDs based on practical experiments, and try our best to reduce the toxicity of Cu-lUDs.     

剑杆织机打纬引纬系统结构尺寸误差对打纬和引纬运动的影响

本文用矩阵法分析了剑杆织机系统结构尺寸误差对打纬和剑头引纬运动的影响,建立了打纬和剑头运动两者的误差与系统各结构参数误差之间关系的矩阵方程。以1511普及型剑杆织机为实例进行了分析计算,得到了较满意的结果。本文所述及的方法具有一般性。     

液氮在弦月形通道中沸腾传热强化实验研究

首次利用极限偏心圆管形成的弦月形通道在液氮池中进行了两相流沸腾传热实验研究，实验结果表明，当热流密度达到９ｋＷ／ｍ＾２时，弦月形通道内的传热温差仅为１．１Ｋ，传热性能达到了目前一致公认的沸腾传热系数最高的烧结多孔表面管的同等水平，而其制造工艺、生产成本和运行操作等方面却大大优于后者。     

编译中的循环优化

讨论了循环优化的目标和循环优化的各种程序变换方法．程序变换可大大减少下标变量的访问时间；消除某些类型的相关，提高软件流水的“深度”；合并多个循环，有利于进行代码压缩．     

可信移动Agent的研究及在分布式网络管理中的应用

为解决分布式网络管理的安全问题,利用移动Agent框架作为底层平台,应用VMC概念将移动Agent和SNMP Agent相融合,构建一种基于可信移动Agent的安全体系结构.实验结果表明,采用此体系结构的网络管理系统能够确保移动Agent的可信性.因此,该体系结构在受到Agent到Agent平台或Agent到Agent攻击情况下的安全性得到评估,证明在网络管理任务中其性能是可信的.     

A forensic method for efficient file extraction in HDFS based on three-level mapping

The large scale and distribution of cloud computing storage have become the major challenges in cloud forensics for file extraction. Current disk forensic methods do not adapt to cloud computing well and the forensic research on distributed file system is inadequate. To address the forensic problems, this paper uses the Hadoop distributed file system (HDFS) as a case study and proposes a forensic method for efficient file extraction based on three-level (3L) mapping. First, HDFS is analyzed from overall architecture to local file system. Second, the 3L mapping of an HDFS file from HDFS namespace to data blocks on local file system is established and a recovery method for deleted files based on 3L mapping is presented. Third, a multi-node Hadoop framework via Xen virtualization platform is set up to test the performance of the method. The results indicate that the proposed method could succeed in efficient location of large files stored across data nodes, make selective image of disk data and get high recovery rate of deleted files.