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701.
Design and application of 60mer oligonucleotide microarray in SARS coronavirus detection 总被引:6,自引:0,他引:6
SHI Rong MA Wenli WU Qinghua ZHANG Bao SONG Yanbin GUO Qiuye XIAO Weiwei WANG Yan & ZHENG Wenling . Institute of Molecular Biology First Military Medical University Guangzhou China . Department of Medical Research Guangzhou Liu Hua Qiao Hospital Guangzhou China Correspondence should be addressed to Ma Wenli 《科学通报(英文版)》2003,48(12):1165-1169
In April 2003, a novel coronavirus[1,2] which was associated with cases of Severe Acute Respiratory Syn-drome (SARS) was first isolated and sequenced in Canada. The genome of SARS coronavirus (SARS-CoV) is 29727[3] nucleotides in length and has 11 known open reading frames (ORFs). Although the genome organiza-tion of this virus is similar to that of other coronaviruses, phylogenetic analyses and sequence alignment show that SARS-CoV is not closely related to any of the previously ch… 相似文献
702.
WANG Yi CHU Wuyang SU Yanjing GAO Kewei & QIAO Lijie Department of Materials Physics University of Science & Technology Beijing Beijing China 《科学通报(英文版)》2003,48(12):1203-1206
It is known that some types of ceramics, like Al2O3 and ZrO2, undergo stress corrosion cracking (SCC) or static fatigue fracture under static loading, ever in a rela-tively mild environment, such as moist atmosphere or water at room temperature, which leads to a time-dependent fracture[1—4]. For a transmission electron microscopy specimen of YBa2Cu3O7-x ceramics superconductor, SCC induced by residual stress can occur in an atmosphere with low moisture content[5]. For a lead zirconate ti… 相似文献
703.
Do multiple cycles of aeolian deposit-pedogenesis exist in the reticulate red clay sections in southern China? 总被引:4,自引:0,他引:4
HU Xuefeng CHENG Tianfan & WU Hexin Department of Environmental Science Engineering Shanghai Uni-versity Shanghai China Correspondence should be addressed to Hu Xuefeng 《科学通报(英文版)》2003,48(12):1251-1258
Since the Quaternary scientists successfully dis-closed the paleoclimatic significance of the loess-paleo- sol sequence in the Loess Plateau, Northwest China, the loess-paleosol sequence has become well-known terres-trial materials revealing the multiple cyclic changes of Quaternary climates. As a carrier of paleoenvironmental information, it could compare beauty with the deep ocean deposit[8]. In recent years, however, the researchers began to put their sights to southern China. They try t… 相似文献
704.
NIEYuchun KEYeyan WANGZai YUXiang DENGHongkui DINGMingxiao 《科学通报(英文版)》2003,48(13):1352-1357
The L protein (241kD) of vesicular stomatitis virus (VSV) is the most important snbnnit of the replication complex. The existence of specific localization signal in the L protein was investigated by making recombinant constructs expressing truncated mutants of the L protein fused to green fluorescent protein (GFP) in transient transfection assays. The chimeric genes encoding varied N-terminal of L and GFP gene were put under the control of T7 promoter or CMV promoter. The fusion proteins were transiently expressed in BHK-21, COS-7, CHO or Hep G2 cells. When more than 120 residues were deleted or only 96 residues were kept on the N-terminal, the fusion proteins were shown to be distributed throughout the cells, cytoplasm and nucleus under the confocal microscope. However, other chimeric proteins with 120 or more amino acids were dotted and distributed in the perinuclear regions. And the fusion protein with 96—120 aa has the similar distribution. A thirteen-residue peptide QGYSFLHEVDKEA (108—120) was identified as localization signal, whose function would be absolutely distributed with the deficiency of D or V. Our results show that there is an independent localizing signal in N-terminal domain of L protein of VSV and this functional signal is conserved in different cell lines. 相似文献
705.
The age of formation of the mirabilite and sand wedges in the Hexi Corridor and their paleoclimatic interpretation 总被引:7,自引:0,他引:7
WANG Nai''''ang ZHANG Jianming CHENG Hongyi GUO Jianying & ZHAO Qiang . College of Earth Environment Sciences Lanzhou University Lanzhou China . Cold Arid Regions Environmental Engineering Research Institute Chinese Academy of Sciences Lanzhou China 《科学通报(英文版)》2003,48(14):1439-1445
The Hexi Corridor is located at the transition zone of the Asian summer monsoon and westerly airflow, and lies in an important position in terms of its ecological fragility and climatic sensitivity. During a recent field expedition to this region, we found a sedimentary mirabilite layer in a number of localities including Suwushan, Yanchi, Baitujing, Yanchi Gaotai, Huahai, Yumen, Halanuo扙r, and Dunhuang (Fig. 1), which have the potential to provide important information about environmenta… 相似文献
706.
YANGBao A.Braeuning SHIYafeng ZHANGJiawu 《科学通报(英文版)》2003,48(14):1446-1450
The paleoclimate data recovered from ice cores,tree rings and lake sediments indicate regional features of cfimatic change on the Tibeta n Plateau (TP) during the last 2000 years. The composite temperature reconstructions in-dicate that several main climatic episodes, such as the “LittleIce Age“ between 1400 and 1900, the “Medieval Warm Pe-riod“ in 1150-1400, a less warm period in 800-1100, and an earlier cold period between the 3rd and 5th centuries,occurred in the TP. In addition, temperature varied from region to region. The period from AD 800 to 1100, which waswarm in northeastern TP, was contemporaneous with cool-ing in the western and southern TP. The southern TP ex-perienced warming between 1150 and 1400. For western TP,the δ^18O records of the Guliya ice core indicate that the pe-Hod 1250-1500 witnessed a clear warming. Large-scaletrends in the temperature history from northeastern TP aremore similar to those in eastern China than are the trendsfrom the Guliya ice cap far to the west and southern TP. The most prominent similarities between the temperature varia-tions of the TP and eastern China are such cold phases as 1100-1150, 1500-1550, 1650-1700 and 1800-1850, andthe latter three cold events match with three widespreadg lacial advances which occurred on the TP during the Little Ice A2e. 相似文献
707.
WUGuang ZHOUChunyuan ZENGHeping 《科学通报(英文版)》2003,48(16):1704-1708
We introduce a stable, long-distance singlephoton Sagnac interferometer, which has a balanced configuration to efficiently compensate phase drift caused by change of the fiber-optic path. By using time-division phase modulation, single-photon interference was realized at 1550nm in a 5-km-long as well as 27-km-long Sagnac fiber loops,with a fringe visibility higher than 90% and long-term stability. The stable performance of the single-photon interference indicated that the time-division phase-modulated Sagnac interferometer might readily lead to practical applications in single-photon routing and quantum cryptography. 相似文献
708.
MAOJinshu WANGXianghui CUIYuanlu YAOKanade 《科学通报(英文版)》2003,48(17):1807-1810
With the development in the field of tissue engineering, the interaction between biomaterials and cells has been deeply studied. Viewing the cells seeded on the surface of materials as an organic whole, cell cycle and apoptosis are analyzed to deepen the study of cell compatibility on biomaterials, while cell proliferation and differentiation are studied at the same time. In this paper, hyaluronic acid is incorporated into the chitosan-gelatin system. Propidium iodide (PI) was used in cell cycle analysis and the double-staining of cells with annexin-V and PI was applied in cell apoptosis analysis. The results show that incorporated hyaluronic acid shortens the adaptation period of cells on the material surface, and then cells enter the normal cell cycle quickly. In addition, added hyaluronic acid inhibits cell apoptosis triggered by the membranes. Therefore, hyaluronic acid improves the cell compatibility of chitosan-gelatin system and benefits the design of biomimetic materials. 相似文献
709.
WANGNing CHENXiaoguang CHENLi YAOJihua CHENHaoming SHENQi XUEJinglun 《科学通报(英文版)》2003,48(19):2061-2063
Human FⅨ expression vector pCMVⅨ was packaged by effectene^TM reagent and injected into mice seminiferous tubules with glass pipettes.The expressional frame of pCMVⅨ was examined by PCR and Southern blotting among 41 progenies.There were 2(4%) mice being integrated with hFⅨ gene into chromosomes.4.6ng/mL of hFⅨ protein was expressed in plasma of one mouse,which was tested by ELISA.We demonstrated that building of transgenic animals by spermatogonial stem cells is an efficient method.Meanwhile,it has also been proved to be an alternative choice for mammary gland bioreactor. 相似文献
710.
LIU Fengxia SUN Chuanqing TAN Lubin FU Yongcai LI Dejun & WANG Xiangkun Department of Plant Genetics Breeding China Agricultural University National Key Laboratory of Agrobiology Key Laboratory of Crop Genetic Improvement Genome of the Ministry of Agriculture Beijing China 《科学通报(英文版)》2003,48(19)
Cold injury is an important limitation of rice production. Therefore, screening for cold-tolerant genetic resources and the development of highly cold-tolerant cultivars is crucial for higher yield potential and stable yield of rice. There have been several reports on the genetic analysis and QTL mapping of the cold-tolerance at the booting stage. Toriyama et al.[1,2] and Futsuhara et al.[3,4] reported that 4 or more genes were involved in the cold-tolerance. Several QTLs at the booting sta… 相似文献