XPDL与关系数据库之间的映射

XPDL( XML Process Definition Language)是工作流管理联盟(WFMC)制定的工作流模型标准,而关系数据库是当前很多工作流厂商存放工作流数据的首选方法.讨论了XPDL与关系数据库(RDB)之间的映射原理及映射方法,并由此建立了映射文件,从而方便了XPDL与RDB之间的双向数据转换.     

Broad phylogenomic sampling improves resolution of the animal tree of life

Long-held ideas regarding the evolutionary relationships among animals have recently been upended by sometimes controversial hypotheses based largely on insights from molecular data. These new hypotheses include a clade of moulting animals (Ecdysozoa) and the close relationship of the lophophorates to molluscs and annelids (Lophotrochozoa). Many relationships remain disputed, including those that are required to polarize key features of character evolution, and support for deep nodes is often low. Phylogenomic approaches, which use data from many genes, have shown promise for resolving deep animal relationships, but are hindered by a lack of data from many important groups. Here we report a total of 39.9 Mb of expressed sequence tags from 29 animals belonging to 21 phyla, including 11 phyla previously lacking genomic or expressed-sequence-tag data. Analysed in combination with existing sequences, our data reinforce several previously identified clades that split deeply in the animal tree (including Protostomia, Ecdysozoa and Lophotrochozoa), unambiguously resolve multiple long-standing issues for which there was strong conflicting support in earlier studies with less data (such as velvet worms rather than tardigrades as the sister group of arthropods), and provide molecular support for the monophyly of molluscs, a group long recognized by morphologists. In addition, we find strong support for several new hypotheses. These include a clade that unites annelids (including sipunculans and echiurans) with nemerteans, phoronids and brachiopods, molluscs as sister to that assemblage, and the placement of ctenophores as the earliest diverging extant multicellular animals. A single origin of spiral cleavage (with subsequent losses) is inferred from well-supported nodes. Many relationships between a stable subset of taxa find strong support, and a diminishing number of lineages remain recalcitrant to placement on the tree.     

嵌入式数据库SQLite在家居能源控制子系统中的应用

针对家居能源控制系统测控点多,实时性和可靠性要求高的特点,提出了使用嵌入式数据库SQLite对系统的批量数据进行管理和维护.本文分别介绍了家居能源控制系统的总体设计,软件设计和数据库设计,以及在嵌入式系统WinCE4.2下SQLite的使用方法,并结合实例具体说明了SQLite在家居能源控制系统中的应用,最后用实验证明了SQLite的优越性.SQLite实现简单,可以提高程序的运行效率,增加程序的稳定性和可读性,因此具有广泛的应用前景.     

以MDR1基因启动子为靶点的药物筛选模型的建立

通过构建以MDR1启动子为启动序列的荧光素酶报告基因载体,建立基于双荧光素酶报告基因系统的多药耐药抑制剂筛选模型。从HCT-8细胞中提取DNA并克隆含有MDR1基因启动子的序列。将该序列重组到荧光素酶报告基因载体pGL-3-Basic的启动区域中,从而构建报告基因载体pGL-MDR1。将pGL-MDR1和pRL-TK载体共转染到HCT-8和HCT-8/VCR细胞中,建立用于筛选多药耐药抑制剂的方法。通过调节不同载体的比例来优化转染效率。通过MDR1基因激活剂（热诱导）和抑制剂（EGCG）来验证该方法。通过直接测序法验证了pGL-MDR1含有MDR1基因启动子序列且没有出现碱基突变。在n（pGL-MDR1）∶n（pRL-TK）=5∶5时,转染效率最高并具有最高的荧光素酶活性。通过MDR1基因激活处理后表现为时间依赖性地激活MDR1基因的表达,而MDR1基因抑制剂的作用则相反。     

The turnover of F-actin-bound ADP in vivo

Summary A procedure for estimating the rate of turnover of F-actin-bound ADP in vivo is described. A turnover rate of 0.88 h^–1 was determined for mouse muscle F-actin. The validity of the method when used to estimate the turnover rate of F-actin per se is discussed in relation to the possible exchange of F-actin-bound ADP.Acknowledgments. The invaluable technical assistance of Mr L. Carrington is gratefully acknowledged.     

新疆主要土壤物理性状空间分布及其气候影响分析

依据新疆33个农业气象观测站（点）2006～2007年实测的0～50em土层土壤水文物理常数资料以及各站多年平均气候资料。综合分析了各常数的空间分布特征、相互间的关系以及各常数与年平均气温、年降水量的统计关系。结果表明,在新疆区域内,1）土壤容重具有南疆〉北疆〉天山山区的分布特征,田间持水量和凋萎湿度具有南疆〈北疆〈天山山区分布格局;2）各土壤水文物理常数两两之间均具有极显著的对数型回归关系,其中,土壤容重与田间持水量、土壤容重与凋萎湿度间呈负相关关系,而田间持水量与凋萎湿度间呈正相关关系;3）各常数与年平均气温和年降水量之间也具有显著的统计关系,其中,土壤容重与气温呈正相关关系,田间持水量、凋萎湿度与气温呈负相关关系;土壤容重与年降水量呈负相关关系,但田间持水量、凋萎湿度与年降水量呈正相关关系。     

一元回归在射表符合系数曲线制作中的应用

针对现有射表符合系数曲线绘制过程中存在的不足，运用一元回归方法对其进行改进，并验证了把该方法用于制作射表符合系数曲线时，可以提高试验数据处理效率以及试验数据的准确度．     

Structure of the insulin receptor ectodomain reveals a folded-over conformation

The insulin receptor is a phylogenetically ancient tyrosine kinase receptor found in organisms as primitive as cnidarians and insects. In higher organisms it is essential for glucose homeostasis, whereas the closely related insulin-like growth factor receptor (IGF-1R) is involved in normal growth and development. The insulin receptor is expressed in two isoforms, IR-A and IR-B; the former also functions as a high-affinity receptor for IGF-II and is implicated, along with IGF-1R, in malignant transformation. Here we present the crystal structure at 3.8 A resolution of the IR-A ectodomain dimer, complexed with four Fabs from the monoclonal antibodies 83-7 and 83-14 (ref. 4), grown in the presence of a fragment of an insulin mimetic peptide. The structure reveals the domain arrangement in the disulphide-linked ectodomain dimer, showing that the insulin receptor adopts a folded-over conformation that places the ligand-binding regions in juxtaposition. This arrangement is very different from previous models. It shows that the two L1 domains are on opposite sides of the dimer, too far apart to allow insulin to bind both L1 domains simultaneously as previously proposed. Instead, the structure implicates the carboxy-terminal surface of the first fibronectin type III domain as the second binding site involved in high-affinity binding.