Building of hFⅨ transgenic mice by spermatogenic cells

Human FⅨ expression vector pCMVⅨ was packaged by effectene^TM reagent and injected into mice seminiferous tubules with glass pipettes.The expressional frame of pCMVⅨ was examined by PCR and Southern blotting among 41 progenies.There were 2(4%) mice being integrated with hFⅨ gene into chromosomes.4.6ng/mL of hFⅨ protein was expressed in plasma of one mouse,which was tested by ELISA.We demonstrated that building of transgenic animals by spermatogonial stem cells is an efficient method.Meanwhile,it has also been proved to be an alternative choice for mammary gland bioreactor.     

Identification and mapping of quantitative trait loci controlling cold-tolerance of Chinese common wild rice (O. rufipogon Griff.) at booting to flowering stages

Cold injury is an important limitation of rice production. Therefore, screening for cold-tolerant genetic resources and the development of highly cold-tolerant cultivars is crucial for higher yield potential and stable yield of rice. There have been several reports on the genetic analysis and QTL mapping of the cold-tolerance at the booting stage. Toriyama et al.[1,2] and Futsuhara et al.[3,4] reported that 4 or more genes were involved in the cold-tolerance. Several QTLs at the booting sta…     

Evolution of the South China Sea and monsoon history revealed in deep-sea records

As the third summary report of ODP Leg 184 to the South China Sea (SCS), this paper discusses the evolution of the East Asian monsoon and the SCS basin. A multi-proxy approach, involving geochemistry, micropale-ontology, pollen and other analyses, was adopted for reconstructing the evolutionary history of the East Asian monsoon, which was characterized by a series of paleo-climate events especially at 8, 3.2, 2.2 and 0.4 Ma. The new record indicates similar stages in the development of the East and South Asian monsoons, with an enhanced winter monsoon over East Asia being the major difference. The rich spectrums of monsoon variability from the southern SCS also reveal other characteristic features of the low latitude ocean. Evidence for the evolution of the SCS includes the hemipelagic Oligocene sediments, implying the existence of deep water environments during the early seafloor spreading stage of the SCS basin. The four major unconformities and some remarkabl ediagenetic features in upper Oligocene deposits indicate the strongest tectonic events in the region. From a careful comparison of lithologies and sedimentation rates, we conclude that the prominent differences in sedimentary environments between the southern and northern SCS were established only by ～3 Ma.     

南海海底特征、资源区位与疆界断续线

根据南海海底的成因与地形特征 ,并通过建立和分析南海海底数字地形模型 ,将南海的海底分为中央的深海盆地、两侧的阶梯状大陆坡、以及周边亚洲大陆延伸的大陆架 3个地貌单元 .再据 194 7年“南海诸岛位置图”精确计算出二战后依据波茨坦公告为国际认可的断续线疆界的经纬度坐标 ,阐述了中国对南海主权的历史延续性 .最后 ,论述了南海区位与资源的优势 ,论证了其海域具体位置与巨大的经济政治战略意义 .     

Inhibitory effect of the adenovirus type 5 E1A protein expressed in the yeast system of the human tumor cell growth

Adenovirus 5 type E1A as a tumor suppressor gene can inhibit tumor growth and enhance the censitivity of chemotherapy and radiotherapy.E1A have the ability to integrate into the host genome,resulting in long-time expres-sion that induces Rb gene inactivation and animal cells im-mortalization.This prompted us to select the E1A protein for treatment of cancer in order to overcome the limitations of E1A gene therapy.Thus,we firstly comstructed E1A eu-caryotic expression vector (pPIC9/E1A),transformated the pichia pastoris yeast cells(GS115) and screened the high-expressing recombinant strains.The positive yeast strains were cultured in the shake flask,and induced for 3d.The crude E1A protein was purified using two steps of col-umu chromatography on HiTrap Q and HiTrap SP.The pu-rified E1A protein was identified by SDS-PAGE and Western blot.E1A protein was mostly located at cellular unclear when Cheriot delivered E1A protein into cells.The analysis in vitro indicated that the E1A protein arrested LN686 cell cycle at G2/M phase,and significantly inhibited the growth of LN686 tumor cells.The current studies firstly provided an experimental basis to further develop E1A protein for tumor treatment.     

有关李群局部生成定理的运用与研究

本由李群局部生成定理出发得出了一些结论，并对李群结构性质作了相关讨论。     

乌鲁木齐市现代化水平现状评价

基于前人的理论与经验 ,本文建立了乌鲁木齐现代化指标体系和标准 ,以 1998～ 2 0 0 0年统计数据为例 ,分析了乌鲁木齐现代化实现现状 ,根据其解析评价了乌鲁木齐现代化建设中主要存在的问题 ,进而提出相应的对策。     

吸水剂造林刍议

论述了吸水剂造林国内外发展概况，吸水剂主要性能及吸水剂造林方法。应用吸水剂造林，可提高土壤的水分含量，保持土壤水分，改善土壤结构，促进土壤中的水、气、热等因子的进一步改善，有利于树木成活和生长发育，提高造林成效。     

太原市发展屋顶绿化应注意的若干问题

屋顶绿化是我国城市建设的一个崭新亮点，它既有增加城市绿地面积、发挥生态林业的作用，又有美化城市、为居民提供活动场所的功能。简要介绍了国内外屋顶绿化的发展状况，分析了太原市发展屋顶绿化的技术难点，提出了地毯式、花槽连网架式、花园式3种屋顶绿化模式，并建议就不同模式进行试点。