CHARACTERISTICS OF QUATERNARY AMMONIUM RESISTANT BACTERIA FROM A FINE PAPERMACHINE SYSTEM

To evaluate the biocide effect of quaternary ammonium chloride (QAC), a whitewater sample was taken from a fine papermachine headbox. By plate spreading method, 51 strains of facultative anaerobe were isolated morphologically. Then the strains were separately transferred to basal medium and were incubated before the beginning of log phase. To identify strains with different QAC resistance, 30-120 ppm of N-Alkyl-benzyl-dimethyl ammonium chloride were added to basal medium.Biocide effect was investigated by comparison of bacterial growth, which can be monitored by 600 nm light absorbance of basal medium suspension. Among 51 strains, only 2 strains can Survive for QAC concentration up to 120 ppm. API20E aud 16S rRNA gene sequencing technique were applied to identify two strains with highest (120 ppm)QAC resistance. One strain (HB22)was identified as Morganella morganii. HB22 can resist QAC concentration up to 150 ppm. HB22 is Gram-negative rod, motile with flagella, catalase positive, oxidase negative, Indole positive, H2S production negative, facultative anaerobe. HB22 has optimum growth condition of 35℃ and pH 7.0.HB22 can catabolize only glucose and D (+)-Mannose.The other (HB45) was identified with high similarity among Pseudomonas cf. monteili or Pseudomonas mosselii or Pseudomonas putida. HB45 can resist QAC concentration up to 200 ppm.HB45 is Gram-negative rod, motile with flagella,catalase positive, oxidase negative, Indole negative,H2S production negative, aerobe. HB45 has optimum growth condition of 30℃ and pH 7.4. HB45 can catabolize L-Arabinose, L(+)-Rhanmose, D (+)-Mannose, Glucose, Glycerol, Lactose, Maltose,Raffmose, Xylose, Cellulose and Xylan. The implication of this work to paper industry is also discussed.     

PIC系列单片机片内定时器实时时钟的实现

针对直接用单片机片内定时器作实时计时不准问题，本文提出了一种可以获取与秒成整数倍关系的定时器溢出信号的方法，同时克服定量溢出中信号被其它中断暂停所受的计时影响，实验表明，在4MHz晶振下，计时误差可控制在每天14s以下，本方法也适用于一般的单片机，在PIC87系列上的实验结果表明效果很好。     

Research on Tracing Evaluation System in Virtual Enterprise Based on Neural Network

The tracing evaluation index system was designed to be used in virtual enterprise and established neural network trace evaluation model. As a result, it was more simple and nicer than the traditional method, so it had wider application foreground.     

共晶体组织对Ag-CdO触头感应钎焊剪切强度的影响

介绍了感应钎焊氧化镉Ag CdO12 触头剪切强度试验结果 ,探讨了组织形态对Ag CdO12 触头感应钎焊强度的影响。结果表明 :当触头钎着率在 75 %以上时 ,触头的剪切强度随着钎缝共晶体组织的减少而增大     

桅杆结构数据采集软件

文章介绍了桅杆结构数据采集软件的编制 ,并基于HY -8021多功能数据采集板与光电位置传感器的特性 ,使得该软件可满足以低频振动为主的建筑工程结构数据采集的要求。     

苏南经济社会发展与高等应用型技术教育对策研究

分析高等应用型技术教育中面临的诸多挑战,探讨行之有效的人才培养对策。     

自主机器人人工进化方法研究

在介绍人工进化研究状况的基础上 ,着重阐述了进化机器人学的关键问题 :基本概念、进化技术、进化计算和实验研究方法等 ,探索了进化机器人学未来的研究趋势及所面临的挑战 .     

山西省吕梁区灌溉用水定额的编制

文章以调查典型县现状年灌溉用水定额为基础 ,以当地灌溉水可利用量为控制条件 ,采用逐级平衡的办法 ,制定吕梁区主要作物的灌溉用水定额     

改进VFP性能的几项实用技术

本文根据作者近几年的教学实践和实际开发经验,从优化、提高VFP性能的目标出发,介绍一些实用技巧和技术,从而大大改进Visual FoxPro的性能,提高数据的访问速度.     

谈家庭害虫的危害及防治

家庭是人们生活居住的重要场所，伴人而居的害虫影响到人们的生活质量。因此，采用环境卫生防治、保管技术防治、化学防治、物理与机械防治、人工捕杀害虫等措施，能够有效地改善家庭生活环境。