全文获取类型
收费全文 | 38880篇 |
免费 | 67篇 |
国内免费 | 107篇 |
专业分类
系统科学 | 184篇 |
丛书文集 | 779篇 |
教育与普及 | 99篇 |
理论与方法论 | 233篇 |
现状及发展 | 17191篇 |
研究方法 | 1540篇 |
综合类 | 18530篇 |
自然研究 | 498篇 |
出版年
2013年 | 245篇 |
2012年 | 523篇 |
2011年 | 1046篇 |
2010年 | 232篇 |
2008年 | 648篇 |
2007年 | 668篇 |
2006年 | 702篇 |
2005年 | 716篇 |
2004年 | 637篇 |
2003年 | 691篇 |
2002年 | 666篇 |
2001年 | 1148篇 |
2000年 | 1055篇 |
1999年 | 731篇 |
1992年 | 696篇 |
1991年 | 557篇 |
1990年 | 602篇 |
1989年 | 595篇 |
1988年 | 599篇 |
1987年 | 612篇 |
1986年 | 578篇 |
1985年 | 737篇 |
1984年 | 584篇 |
1983年 | 494篇 |
1982年 | 444篇 |
1981年 | 434篇 |
1980年 | 572篇 |
1979年 | 1214篇 |
1978年 | 1031篇 |
1977年 | 1020篇 |
1976年 | 757篇 |
1975年 | 823篇 |
1974年 | 1127篇 |
1973年 | 1008篇 |
1972年 | 1066篇 |
1971年 | 1256篇 |
1970年 | 1631篇 |
1969年 | 1259篇 |
1968年 | 1211篇 |
1967年 | 1239篇 |
1966年 | 1059篇 |
1965年 | 754篇 |
1964年 | 191篇 |
1959年 | 447篇 |
1958年 | 683篇 |
1957年 | 541篇 |
1956年 | 456篇 |
1955年 | 397篇 |
1954年 | 424篇 |
1948年 | 269篇 |
排序方式: 共有10000条查询结果,搜索用时 0 毫秒
761.
Biological cells harbor a variety of molecular machines that carry out mechanical work at the nanoscale. One of these nanomachines
is the bacterial motor protein SecA which translocates secretory proteins through the protein-conducting membrane channel
SecYEG. SecA converts chemically stored energy in the form of ATP into a mechanical force to drive polypeptide transport through
SecYEG and across the cytoplasmic membrane. In order to accommodate a translocating polypeptide chain and to release transmembrane
segments of membrane proteins into the lipid bilayer, SecYEG needs to open its central channel and the lateral gate. Recent
crystal structures provide a detailed insight into the rearrangements required for channel opening. Here, we review our current
understanding of the mode of operation of the SecA motor protein in concert with the dynamic SecYEG channel. We conclude with
a new model for SecA-mediated protein translocation that unifies previous conflicting data. 相似文献
762.
Yvonne G. J. van Helden Roger W. L. Godschalk Hans J. M. Swarts Peter C. H. Hollman Frederik J. van Schooten Jaap Keijer 《Cellular and molecular life sciences : CMLS》2011,68(3):489-504
Molecular mechanisms triggered by high dietary beta-carotene (BC) intake in lung are largely unknown. We performed microarray
gene expression analysis on lung tissue of BC supplemented beta-carotene 15,15′-monooxygenase 1 knockout (Bcmo1
−/−) mice, which are—like humans—able to accumulate BC. Our main observation was that the genes were regulated in an opposite direction in male and female Bcmo1
−/− mice by BC. The steroid biosynthetic pathway was overrepresented in BC-supplemented male Bcmo1
−/− mice. Testosterone levels were higher after BC supplementation only in Bcmo1
−/− mice, which had, unlike wild-type (Bcmo1
+/+) mice, large variations. We hypothesize that BC possibly affects hormone synthesis or metabolism. Since sex hormones influence
lung cancer risk, these data might contribute to an explanation for the previously found increased lung cancer risk after
BC supplementation (ATBC and CARET studies). Moreover, effects of BC may depend on the presence of frequent human BCMO1 polymorphisms, since these effects were not found in wild-type mice. 相似文献
763.
The skin is our primary shield against microbial pathogens and has evolved innate and adaptive strategies to enhance immunity
in response to injury or microbial insult. The study of antimicrobial peptide (AMP) production in mammalian skin has revealed
several of the elegant strategies that AMPs use to prevent infection. AMPs are inducible by both infection and injury and
protect the host by directly killing pathogens and/or acting as multifunctional effector molecules that trigger cellular responses
to aid in the anti-infective and repair response. Depending on the specific AMP, these molecules can influence cytokine production,
cell migration, cell proliferation, differentiation, angiogenesis and wound healing. Abnormal production of AMPs has been
associated with the pathogenesis of several cutaneous diseases and plays a role in determining a patient’s susceptibility
to pathogens. This review will discuss current research on the regulation and function of AMPs in the skin and in skin disorders. 相似文献
764.
Tin oxide (SnO2) nanoparticles were cost-effectively synthesized using nontoxic chemicals and green tea (Camellia sinensis) extract via a green synthesis method.The structural properties of the obtained nanoparticles were studied using X-ray diffraction,which indicated that the crystallite size was less than 20 nm.The particle size and morphology of the nanoparticles were analyzed using scanning electron microscopy and transmission electron microscopy.The morphological analysis revealed agglomerated spherical nanoparticles with sizes varying from 5 to 30 nm.The optical properties of the nanoparticles' band gap were characterized using diffuse reflectance spectroscopy.The band gap was found to decrease with increasing annealing temperature.The O vacancy defects were analyzed using photoluminescence spectroscopy.The increase in the crystallite size,decreasing band gap,and the increasing intensities of the UV and visible emission peaks indicated that the green-synthesized SnO2 may play future important roles in catalysis and optoelectronic devices. 相似文献
765.
The aim of this study was to determine the structural, compositional, and mineralogical composition of carbonatitic copper sulfide concentrator plant streams. Three samples, each from a different stream(run of mine(ROM), concentrate, and tailings) of a copper concentrator were characterized using various techniques, including stereomicroscopy, X-ray fluorescence, X-ray diffraction, Fourier transform infrared(FTIR) spectroscopy, scanning electron microscopy(SEM) in conjunction with energy-dispersive X-ray spectroscopy(EDS), and optical microscopy. The results reveal that each stream possesses its own unique compositional features. Carbonate minerals associated with calcite and dolomite, followed by quartz, remain the major minerals in both the ROM and tails streams. In the ROM stream, chalcopyrite appears to occur as veins within the carbonatite-hosting ore body. Mineral phase mutation was discovered in the tails stream because magnetite formerly identified in the ROM as the primary iron oxide had evolved into hematite. This metamorphosis was likely promoted by the concentration process. The concentration process was effective, upgrading the chalcopyrite content from 2 wt% in the ROM stream to 58 wt% in the concentrate stream; it was accompanied by bornite(4 wt%), anilite(3 wt%), and digenite(2.5 wt%). In addition, the concentrate stream exhibited properties distinctive from those of the other streams. The FTIR analysis showed the existence of a sulfide group related to the chalcopyrite mineral. Free chalcopyrite grains were observed in the concentrate by SEM analysis, and their mineral presence was supported by the EDS analysis results. All characterization techniques corresponded well with each other regarding the structure, chemistry, and composition of the samples. 相似文献
766.
767.
Respiration as the main determinant of carbon balance in European forests 总被引:106,自引:0,他引:106
Valentini R Matteucci G Dolman AJ Schulze ED Rebmann C Moors EJ Granier A Gross P Jensen NO Pilegaard K Lindroth A Grelle A Bernhofer C Grünwald T Aubinet M Ceulemans R Kowalski AS Vesala T Rannik U Berbigier P Loustau D Gudmundsson J Thorgeirsson H Ibrom A Morgenstern K Clement R 《Nature》2000,404(6780):861-865
Carbon exchange between the terrestrial biosphere and the atmosphere is one of the key processes that need to be assessed in the context of the Kyoto Protocol. Several studies suggest that the terrestrial biosphere is gaining carbon, but these estimates are obtained primarily by indirect methods, and the factors that control terrestrial carbon exchange, its magnitude and primary locations, are under debate. Here we present data of net ecosystem carbon exchange, collected between 1996 and 1998 from 15 European forests, which confirm that many European forest ecosystems act as carbon sinks. The annual carbon balances range from an uptake of 6.6 tonnes of carbon per hectare per year to a release of nearly 1 t C ha(-1) yr(-1), with a large variability between forests. The data show a significant increase of carbon uptake with decreasing latitude, whereas the gross primary production seems to be largely independent of latitude. Our observations indicate that, in general, ecosystem respiration determines net ecosystem carbon exchange. Also, for an accurate assessment of the carbon balance in a particular forest ecosystem, remote sensing of the normalized difference vegetation index or estimates based on forest inventories may not be sufficient. 相似文献
768.
769.
Most recently produced wind generators use variable-speed systems for high-efficiency operation and low mechanical stress.In this study of a variable-speed standalone system that uses a synchronous machine,a power converter for the system was developed and tested.The maximum power points were approximated in a line according to the wind speed changes,and the linear approximation MPPT algorithm was used to determine the duty ratio that corresponds to the intersection point of the observed power and linearized line using the power generated from the generator at a point.With the fabricated converter,it was verified that the voltage,current and theta were normal with the varied load current. 相似文献
770.
Mechanism of closure of the aortic valve 总被引:5,自引:0,他引:5