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991.
尹良红 《暨南大学学报(自然科学与医学版)》1998,(6)
比较Verapamil和Propranolol对患有胰岛素非依赖型糖尿病及轻度至中度高血压的透析患者的血糖和物质代谢的影响。方法:46例患有非胰岛素依赖型糖尿病及轻度至中度高血压的透析患者,为德国福尔达市KfH血透中心和柏林KfH血透中心的透析病人。分别经Verapamil和Propranolol治疗三个月后测空腹血糖、血红蛋白A1c、果酸、胰岛素、胆固醇和甘油三脂,同时记录透析前心率和血压。结果:Verapamil对血糖及物质代谢无不良影响,Propranolol可引起血糖、果酸和血红蛋白A1c升高。结论:在糖尿病透析患者,Propranolol具有引起和/或加重小动脉血管病病以及脑血管意外的危险性 相似文献
992.
993.
Structure of a human common cold virus and functional relationship to other picornaviruses 总被引:29,自引:0,他引:29
M G Rossmann E Arnold J W Erickson E A Frankenberger J P Griffith H J Hecht J E Johnson G Kamer M Luo A G Mosser 《Nature》1985,317(6033):145-153
We report the first atomic resolution structure of an animal virus, human rhinovirus 14. It is strikingly similar to known icosahedral plant RNA viruses. Four neutralizing immunogenic regions have been identified. These, and corresponding antigenic sequences of polio and foot-and-mouth disease viruses, reside on external protrusions. A large cleft on each icosahedral face is probably the host cell receptor binding site. 相似文献
994.
G. Görgen C. Frößl W. Boland K. Dettner 《Cellular and molecular life sciences : CMLS》1990,46(7):700-704
Summary The terminally unsaturated hydrocarbons of the defensive secretion ofTribolium confusum are biosynthesized from fatty acids by oxidative decarboxylation. The process involves an enantiospecific cleavage of the C–H bond of thepro-(S) C(3)–H atom and simultaneous decarboxylation of the acid into an 1-alkene and carbon dioxide via ananti-periplanar transition state geometry (anti-elimination). The stereochemistry of this biotranformation is identical in all respects with the same reaction in higher plants. The mechanism seems to be of general importance for the biosynthesis of many vinylic substructures of natural products from oxygen-containing precursors. 相似文献
995.
W. Roszkowski J. Beuth H. L. Ko G. Uhlenbruck G. Pulverer 《Cellular and molecular life sciences : CMLS》1989,45(6):584-588
Summary Adhesion and inhibition experiments with pulmonary cells of BALB/c-mouse origin and syngeneic sarcoma L-1 cells indicated that L-fucose specific lectin-like adhesion molecules, presumably situated on pulmonary cell surfaces are (at least partly) responsible for the specificity of this cell-cell interaction. Addition of specific sugars and glycoconjugates (L-fucose and fucoidan, respectively) to the incubation medium evidently inhibited the adhesion process as quantified using radiolabelled tumor cells. Unspecific carbohydrates (e.g. D-galactose) did not affect the cellular interaction. In vivo, repeated administration of fucoidan (but not of unspecific glycoconjugates) significantly inhibited the settling of metastatic sarcoma L-1 cells in the lungs of BALB/c-mice. Therefore, when lectin-like adhesion molecules on pulmonary cells were blocked with competitive glycoconjugates, tumor cell colonization of the lung could be significantly inhibited. 相似文献
996.
The atrial and ventricular myosin light chains of human, monkey and sheep hearts were compared by dodecylsulfate polyacrylamide gel electrophoresis. The atrial light chain 2 and ventricular light chain 2 are similar among these mammals. However, the atrial light chain 1 of monkey has different electrophoretic mobility from those of human and sheep. The monkey ventricular light chain 1 has same mobility as that of sheep but different from that of human. 相似文献
997.
Glenn W. Milligan 《Journal of Classification》1989,6(1):53-71
De Soete (1986, 1988) proposed a variable weighting procedure when Euclidean distance is used as the dissimilarity measure with an ultrametric hierarchical clustering method. The algorithm produces weighted distances which approximate ultrametric distances as closely as possible in a least squares sense. The present simulation study examined the effectiveness of the De Soete procedure for an applications problem for which it was not originally intended. That is, to determine whether or not the algorithm can be used to reduce the influence of variables which are irrelevant to the clustering present in the data. The simulation study examined the ability of the procedure to recover a variety of known underlying cluster structures. The results indicate that the algorithm is effective in identifying extraneous variables which do not contribute information about the true cluster structure. Weights near 0.0 were typically assigned to such extraneous variables. Furthermore, the variable weighting procedure was not adversely effected by the presence of other forms of error in the data. In general, it is recommended that the variable weighting procedure be used for applied analyses when Euclidean distance is employed with ultrametric hierarchical clustering methods. 相似文献
998.
Light-dependent antibody labelling of photoreceptors 总被引:1,自引:0,他引:1
Monoclonal antibodies are tools widely used to analyse the structure of the nervous system. Whereas some labelling patterns are highly reproducible, others appear to vary from one preparation to the next, as we noticed in particular for some antibodies with respect to photoreceptor labelling. To establish whether some of this variability can be linked to functional criteria, we tested for light-dependence. We found two antibodies that label photoreceptor outer segments, only when the retina has been illuminated, and a third antibody that has a selective affinity for dark-adapted outer segments. The two antibodies against light-activated sites are primarily directed against the highly phosphorylated neurofilament subunit at relative molecular mass 200,000 (200K). One of them, RT97, recognizes on immunoblots, in addition to neurofilaments, a light-activated epitope on a protein that resembles the photopigment rhodopsin, presumably a phosphorylation-dependent site. Antibodies like those described here may allow the study of physiological processes such as light and dark adaptation using morphological techniques. 相似文献
999.
Suppression of c-ras transformation by GTPase-activating protein 总被引:27,自引:0,他引:27
The ras genes are required for normal cell growth and mediate transformation by oncogenes encoding protein tyrosine kinases. Normal ras can transform cells in vitro and in vivo, but mutationally activated ras does so much more efficiently, and highly transforming mutant versions of ras have been isolated from a variety of human and animal tumours. The ras genes encode membrane-associated, guanine nucleotide-binding proteins that are active when GTP is bound and inactive when GDP is bound. The slow intrinsic GTPase activity of normal mammalian Ras proteins can be greatly accelerated by the GTPase-activating protein (GAP), which is predominantly cytoplasmic. This activity of GAP, which can increase with cell density in contact-inhibited cells, suggests that it functions as a negative, upstream regulator of ras. Other studies, however, show that GAP interacts with a region of ras-encoded protein implicated in ras effector function, which raises the possibility that GAP might also be a downstream target of ras. Mutationally activated ras-encoded proteins also interact with GAP, although they are resistant to its catalytic activity. In an attempt to define the role of GAP in ras-mediated transformation, we examined the effects on transformation of normal or mutant ras when cells overexpress GAP. We found that GAP suppresses transformation of NIH 3T3 cells by normal Ha-ras (c-ras) but does not inhibit transformation by activated Ha-ras (v-ras). These results support the hypothesis that GAP functions as a negative regulator of normal ras and make it unlikely that GAP alone is the ras target. 相似文献
1000.
Summary Two new C-18-oxygenated sterols possessing a spiroketal function (1 and2) have been isolated from a soft coral of the genusSinularia. The proposed structures are based on spectral data. 相似文献