An Action Design Research Approach within Enterprise Engineering

Enterprise engineering (EE) is emerging as a new discipline that is multi-disciplinary in nature. As highlighted by researchers within the EE discipline, the current status of EE endeavours as taken by several universities is unclear, which led to several initiatives and publications to develop a research agenda within the enterprise engineering research community. This article builds upon existing work aimed at establishing EE as a discipline, also accepting the epistemological stance of idealism, pragmatism and existential phenomenology as argued by Hoogervorst as an appropriate stance for EE research, prior to suggesting action design research (ADR) as an appropriate research method for EE research. More so, the article presents an Action Design Research within Enterprise Engineering (ADR-in-EE) approach as the main contribution to guide prospective EE researchers towards research within the EE discipline. The ADR-in-EE approach is based on ADR, but provides additional guidance by incorporating the use of an Enterprise Evolution Contextualisation Model, as well as creativity facilitation in the form of Univation’s brainstorming method. As a second contribution, we experiment with the ADR-in-EE approach and use a survey to extract feedback on the usefulness of the approach. The research findings are mostly positive, with qualitative feedback on further improving ADR-in-EE approach.     

Recombination of integral and peripheral protein fractions from human red cell membrane with homologous lipids

Summary Integral and peripheral protein fractions from human red cell membranes were recombined with a total red cell lipid extract and with homologous lipids in varying mixtures, by dialysis from 2-chlorethanol solutions. The 2 protein fractions were compared for lipid binding capacity and for selectivity towards individual lipids.     

The Combinatorial Approach to Asymmetric Hydrogenation： More Miles out of Monophos

1 Introduction In custom manufacturing time-to-market is of overriding importance. Thus, asymmetric hydrogenation will be applied only if an appropriate catalyst can be found in a very short period of time. For this reason we have build up a full-scale capability for High Throughput Experimentation, encompassing several robot systems. As ligand synthesis was the last remaining barrier we have explored, together with the University of Groningen, the use of simple monodentate ligands, such as …     

The DNA helicase BRIP1 is defective in Fanconi anemia complementation group J

The protein predicted to be defective in individuals with Fanconi anemia complementation group J (FA-J), FANCJ, is a missing component in the Fanconi anemia pathway of genome maintenance. Here we identify pathogenic mutations in eight individuals with FA-J in the gene encoding the DEAH-box DNA helicase BRIP1, also called FANCJ. This finding is compelling evidence that the Fanconi anemia pathway functions through a direct physical interaction with DNA.     

Heterozygous mutations of the kinesin KIF21A in congenital fibrosis of the extraocular muscles type 1 (CFEOM1)

Congenital fibrosis of the extraocular muscles type 1 (CFEOM1; OMIM #135700) is an autosomal dominant strabismus disorder associated with defects of the oculomotor nerve. We show that individuals with CFEOM1 harbor heterozygous missense mutations in a kinesin motor protein encoded by KIF21A. We identified six different mutations in 44 of 45 probands. The primary mutational hotspots are in the stalk domain, highlighting an important new role for KIF21A and its stalk in the formation of the oculomotor axis.     

Mycobacterium leprae-specific protein antigens defined by cloned human helper T cells

Leprosy displays a remarkable spectrum of symptoms correlating with the T-cell-mediated immune reactivity of the host against the causative organism, Mycobacterium leprae. At one pole of this spectrum are lepromatous leprosy patients showing a M. leprae-specific T-cell unresponsiveness; at the other are tuberculoid leprosy patients displaying both acquired immunity and delayed-type hypersensitivity against M. leprae which are thought to be conferred by helper T (Th) cells. Because well-defined M. leprae antigens are crucial for the prevention and control of leprosy, we have cloned M. leprae-reactive T cells (TLC) of the helper phenotype from a tuberculoid leprosy patient. As reported here, these TLC show an unexpected diversity in the recognition of M. leprae and related mycobacteria, which is different from that exhibited by monoclonal antibodies. Half of these TLC are completely or almost M. leprae-specific, whereas the other half are cross-reactive with most or all other mycobacteria. A M. leprae protein of relative molecular mass (Mr) 36,000 (36K) defined by a M. leprae-specific monoclonal antibody stimulates 4 out of 6 TLC tested. Each of these TLC recognizes a different antigenic determinant, one of which is M. leprae-specific. The previous paper describes other M. leprae-specific T-cell clones half of which recognize an epitope on a M. leprae protein of Mr 18 K.