PRRSV主要蛋白结构分析及其抗原表位的研究

【目的】对猪繁殖与呼吸综合征病毒(PRRSV)主要蛋白,即GP5,GP4,GP3,GP2的二级结构进行分析.【方法】在线利用DNA Star软件分析GP2~GP5蛋白的抗原表位,以指导未来的疫苗设计.【结果】:1GP2~GP5的理论等电点PI值分别为10.12,8.42,8.05,8.41,GP2的PI值明显高于GP3~GP5的PI值(p0.05),而在GP3~GP5之间没有明显差异(p0.05);2GP4蛋白和GP5蛋白高抗原指数的基序数量多,形成抗原表位的可能性最大,是设计疫苗的重要靶点.【结论】高致病PRRSV JXA1株的GP4蛋白和GP5蛋白高抗原指数的基序数量多,形成抗原表位的可能性最大,是设计疫苗的重要靶点.     

浅谈医学科技论文中参考文献的重要性

参考文献作为医学科技论文的重要组成部分，不仅为其论点提供科学依据，而且还使论文内容精练、结构紧凑，同时参考文献的著录又可反映出作者的科技道德和科研水平。因此，无论是作者还是编辑都应对参考文献的著录引起重视。     

高能~(12)C~(6+)离子辐照诱发小肠急性损伤研究

将昆明鼠固定全身接受单能12C6+离子束辐照,于照后不同时间颈椎脱臼法处死小鼠,剖取空肠组织,Bouin固定液固定,石蜡切片切为5μm,HE染色后观察病理变化并统计隐窝部位辐照诱导的细胞凋亡率以及凋亡细胞和增殖细胞在隐窝中的分布.辐照后15.5 h绒毛开始脱落,绒毛密度于25.5 h降至最低,3.5天恢复至正常密度.于照后5.5～6天固有层和肌肉层有炎症细胞浸润,同时固有层淋巴小结增多.小肠黏膜完整性丧失程度与辐照剂量相关.照后5～6天出现隐窝分裂现象.小肠及结肠组织出现类似于绒毛腺瘤的结构.隐窝细胞凋亡率于照后5.5h达到峰值,凋亡细胞最常见于细胞位置10,而增殖细胞最常见于细胞位置16.     

Feasibility Study of Aluminum Hydroxide Sol as Adjuvant to Prepare Stable Vaccine Using Newcastle Disease Virus as a Model

Aluminum hydroxide sol (consisting of aluminum hydroxide nano-particles) prepared by a hydrothermal method was used as nano-aluminum adjuvant adsorbed the inactived Newcastle disease virus (NDV). The zeta potential of aluminum hydroxide colloidal particles,the inactivated NDV and the nano-aluminum adjuvant NDV are +48.16 mV,?13.8 mV and +39.97 mV,respec-tively. The nano-aluminum adjuvant vaccine was transparent and stable without precipitate,whereas the conventional aluminum ad-juvant vaccine was turbid and a white precipitate was visible soon. The hemagglutination inhibition (HI) titers of the nano-aluminum adjuvant group were generally higher than those of the conventional aluminum group except the titer on day 29. The results show that the nano-aluminum adjuvant has better stability and higher levels of antibodies for a longer time than the conventional aluminum adjuvant.     

Carboxymethytl Pachymaram Up-Regulates Dendritic Cell's Function in Hepatitis B Virus Transgenic Mice in vitro

The effect of carboxymethytl pachymaram ( CMP ) on the function of dendritic cells(DCs) derived from spleens of hepatitis B virus transgenic mice are studied in vitro. The phenotypes of DCs are tested by flow cytometry (FCM), cytokines measured by ELISA. The expression of DCs' phenotypes in HBV transgenic mice are low (CD80~ CD11c~ :59.12±11.53 vs 9.60±4.53, p<0.01; CD80 MHC-Ⅱ~ : 44.86±12.31 vs 9.80±5.72, p<0.01, normal mice vs HBV transgenic mice), the ability of DCs stimulating T lymphocytes proliferation decreases (0.37±0.11 vs 0.20±0.11, p<0.05, normal mice vs HBV transgenic mice), levels of IL-12 and IFN-γ decrease whereas the level of IL-10 increases; CMP can enhance DCs' ability of stimulating T lymphocytes proliferation, facilitate the secretion of IL-12 and IFN-γ, inhibit the secretion of IL-10, thus up regulates DCs function. The results show a good prospective use of CMP on the treatment of chronic hepatitis B.     

成年健康犬超声心动图参数测定

本文报告了４２只健康成年犬的Ｍ型、二维与多普勒超声心动图测定值。分析了性别、体重因素对３３项检测参数的影响。目的：为研究犬疾病模型提供正常对照数据。结果显示：性别对形态学参数影响不大，对血流动力学指标影响较大；主要参数随体重变化呈现相关性；解剖形态学较血注动力学指标更趋稳定。     

SPF鸡动物实验室的建立及其实验应用

ＳＰＦ鸡动物实验室的建立及其实验应用范书曦，周蛟，郭志弘，姚炜光，闫乃颐，苏凤林，刘有昌北京市农林科学院畜牧兽医研究所前言随着科研水平的不断提高，对实验动物以及饲养实验动物的环境、设施的要求也有了全新的内容和根本的差别。我所根据“北京市实验动物饲养与...     

近代感染性心内膜炎的特征(病案示例)

本文就近代感染性心内膜炎的特征作了讨论。认为:IE(Infective endocarditis)可以不发热。心脏杂音变化并非IE所独有,除非有瓣膜穿孔或腱索断裂,亚急性心内膜炎的杂音很少在短时间内发生变化,且杂音变化受心外因素影响,右心内膜炎时可听不到杂音。重要器官的栓塞征仍较多,年轻人出现偏瘫或心肌梗塞应考虑IE的可能。IE还可并发各型肾小球肾炎,出现蛋白尿或血尿、氮质血症,蛋白尿较血尿更多见,持续时间更长。实验室检查,一般血培养阳性率低,未梢血中吞噬型网状内皮细胞或单核细胞可增多,白细胞涂片发现含细菌的白细胞,对血培养阴性病人有很高诊断价值。超声心动图检查,大大提高了IE检出率,达75％。可显示瓣膜赘生物(2mm),瓣膜及附件结构的破坏性改变以及由此导致的血流动力学改变。     

两种亲和色谱在中草药活性成分分离和纯化中的应用

亲和色谱多用于生物大分子的分离与纯化。随着技术的发展,出现了多种新型的亲和色谱,其应用范围也不断拓展。本文简要介绍了分子烙印亲和色谱和免疫亲和色谱的基本原理,以及它们在中草药活性成分分离和纯化中的应用概况。     

Diagnosis of Vocal Cord Paralysis in Anaesthesia

Vocal cord paralysis can occur as a complication o surgery or anaesthesia,if permanent is a significant clinica problem.Early detection is important to optimize the chance o repair,and avoid complications associated with an impaired swallow.An algorithm to detect altered vocal cord function was presented based on wavelet packet analysis(WPA) and suppor vector machines(SVM),and compared with the Hoarseness Diagram method(HDm),which was reported as an objective voice quality evaluation approach and could be u...