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71.
E Gottfried C Strolenberg F Van Herp 《Comptes rendus des séances de l'Académie des sciences. Série D, Sciences naturelles》1977,284(1):57-59
Following a serotonin injection, a rise of exocytosis in the sinus gland of the crayfish Astacus leptodactylus is observed together with an increase of the glucose level in the hemolymph. The phenomenon of exocytosis reaches a peak between 2 and 4 h after injection, wheras the highest glucose level in the hemolymph appears between 4 and 6 h after the same injection. 相似文献
72.
Cloning and expression of a rat D2 dopamine receptor cDNA 总被引:24,自引:0,他引:24
J R Bunzow H H Van Tol D K Grandy P Albert J Salon M Christie C A Machida K A Neve O Civelli 《Nature》1988,336(6201):783-787
Dopamine receptors are classified into D1 and D2 subtypes on the basis of their pharmacological and biochemical characteristics. The D2 dopamine receptor has been implicated in the pathophysiology and treatment of movement disorders, schizophrenia and drug addiction. The D2 dopamine receptor interacts with guanine nucleotide-binding proteins to induce second messenger systems. Other members of the family of receptors that are coupled to G proteins share a significant similarity in primary amino-acid sequence and exhibit an archetypical topology predicted to consist of seven putative transmembrane domains. We have taken advantage of the expected nucleotide sequence similarities among members of this gene family to isolate genes coding for new receptors. Using the hamster beta 2-adrenergic receptor gene as a hybridization probe we have isolated related genes including a cDNA encoding the rat D2 dopamine receptor. This receptor has been characterized on the basis of three criteria: the deduced amino-acid sequence which reveals that it is a member of the family of G-protein-coupled receptors; the tissue distribution of the mRNA which parallels that of the D2 dopamine receptor; and the pharmacological profile of mouse fibroblast cells transfected with the cDNA. 相似文献
73.
74.
75.
Sequence of cDNA encoding human insulin-like growth factor I precursor 总被引:10,自引:0,他引:10
M Jansen F M van Schaik A T Ricker B Bullock D E Woods K H Gabbay A L Nussbaum J S Sussenbach J L Van den Brande 《Nature》1983,306(5943):609-611
Somatomedins (SM) or insulin-like growth factors (IGF) constitute a heterogeneous group of peptides with important growth-promoting effects in vitro as well as in vivo. Amino acid sequences have been determined for only two of them, IGF-I and IGF-II, which are highly homologous. IGF-I, which is identical with SM-C, is composed of 70 amino acid residues and IGF-II contains 73 amino acids and may be identical with SM-A. Other peptides with different charge properties but with similar SM-like or insulin-like behaviour in biological and receptor assays, have been described but have not yet been fully characterized. The liver is known to be a major site of production of these peptides, but many other tissues--especially in the fetus--may synthesize them as well. We report here the nucleotide sequence of a human liver cDNA encoding the complete amino acid sequence of IGF-I. The IGF-I coding region is flanked by sequences encoding an amino-terminal peptide of at least 25 amino acid residues and a carboxyl-terminal peptide of 35 amino acids. This provides evidence that IGF-I is synthesized as a precursor protein and that formation of IGF-I from this precursor requires proteolytic processing at both ends. 相似文献
76.
M. Protiva M. Rajšner V. Trčka M. Vaněček Z. J. Vejdělek 《Cellular and molecular life sciences : CMLS》1959,15(2):54-55
Summary A series of cyclohexylamine derivatives methylated both on nitrogen atom and in position 1, 2 and 6 of the cyclohexane ring
have been prepared. Some of them showed considerable hypotensive and ganglioplegic activity. The most interesting compound
is N,N,1,2,2-pentamethylcyclohexylamine (‘penhexamine’) whose activity compares favourably with mecamylamine or dimecamine.
9. MitteilungGanglioplegica, 8. Mitteilung siehe Českoslov. farm:8 (1959), im Druck. 相似文献
9. MitteilungGanglioplegica, 8. Mitteilung siehe Českoslov. farm:8 (1959), im Druck. 相似文献
77.
78.
79.
Summary and conclusions The recent characterization of the human insulin receptor structure and its intrinsic tyrosine kinase activity represent major advances in our understanding of the mechanism of insulin action. It is reasonable to think that the insulin-induced autophosphorylation and activation of its receptor kinase represent an important event in the action of insulin on cell metabolism and growth. The fundamental research reviewed may be followed by the discovery of molecular receptor defects in clinical syndromes of insulin resistance. 相似文献
80.
S. J. Kleinschuster H. J. Rapp A. V. Johnston K. R. Van Kampen C. C. Muscoplat J. Bier 《Cellular and molecular life sciences : CMLS》1980,36(10):1239-1240
Summary A simple method for the anchorage-dependent culture of line 10 guinea-pig hepatoma cells is described. 相似文献