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151.
G. F. Van Went 《Cellular and molecular life sciences : CMLS》1978,34(3):324-325
Summary Using the micronucleus test as a screening method for mutagenic activity, no significant increase in the number of micronuclei was found when LSD, psilocybin or 9-THC were administered in 3 logarithmically increasing doses to mice. Azathioprine (Imuran®), given as a positive control, caused a statistically significant increase in micronucleated cells.Acknowledgment. The expert technical assistance of Mr Joop Branger is greatfully acknowledged. We thank Prof. Dr C. A. Salemink, University of Utrecht, for the supply of the THC, and Dr J. Fokkens, National Institute of Public Health, for preparing the solutions. 相似文献
152.
E. L. Van Alstyne Celia D. Sladek D. C. Smith 《Cellular and molecular life sciences : CMLS》1977,33(7):863-864
Summary The plasma concentration of L-carnitine in scalded rats was determined to be greater (p0.05) than that of control rats at 6 h following the administration of a 20% body surface, full-thickness burn produced by scalding in a 100°C water bath for 15 sec. 相似文献
153.
H. J. Wynne K. H. Van Buuren W. Wakelkamp 《Cellular and molecular life sciences : CMLS》1982,38(6):655-657
Summary Capacity factors in reversed-phase HPLC and distribution constants in octan-l-ol/water of N-acetyl-N-methylamino acid amides have been measured as a function of temperature. The HPLC capacity factors are proposed as estimates of the hydrophobicity of the amino acid side chains.We acknowledge the interest in this work of Dr H. van Rooy, Dr J. Kinkel, Prof. W. Soudijn and Prof. E. Tomlinson. 相似文献
154.
155.
156.
Genome-wide association defines more than 30 distinct susceptibility loci for Crohn's disease 总被引:2,自引:0,他引:2
Barrett JC Hansoul S Nicolae DL Cho JH Duerr RH Rioux JD Brant SR Silverberg MS Taylor KD Barmada MM Bitton A Dassopoulos T Datta LW Green T Griffiths AM Kistner EO Murtha MT Regueiro MD Rotter JI Schumm LP Steinhart AH Targan SR Xavier RJ;NIDDK IBD Genetics Consortium Libioulle C Sandor C Lathrop M Belaiche J Dewit O Gut I Heath S Laukens D Mni M Rutgeerts P Van Gossum A Zelenika D Franchimont D Hugot JP de Vos M Vermeire S 《Nature genetics》2008,40(8):955-962
Several risk factors for Crohn's disease have been identified in recent genome-wide association studies. To advance gene discovery further, we combined data from three studies on Crohn's disease (a total of 3,230 cases and 4,829 controls) and carried out replication in 3,664 independent cases with a mixture of population-based and family-based controls. The results strongly confirm 11 previously reported loci and provide genome-wide significant evidence for 21 additional loci, including the regions containing STAT3, JAK2, ICOSLG, CDKAL1 and ITLN1. The expanded molecular understanding of the basis of this disease offers promise for informed therapeutic development. 相似文献
157.
Vastiau IM Anthonio EA Brams M Brees C Young SG Van de Velde S Wanders RJ Mannaerts GP Baes M Van Veldhoven PP Fransen M 《Cellular and molecular life sciences : CMLS》2006,63(14):1686-1699
Pex19p exhibits a broad binding specificity for peroxisomal membrane proteins (PMPs), and is essential for the formation of
functional peroxisomal membranes. Pex19p orthologues contain a C-terminal CAAX motif common to prenylated proteins. In addition, Saccharomyces cerevisiae and Chinese hamster Pex19p are at least partially farnesylated in vivo. Whether farnesylation of Pex19p plays an essential or merely ancillary role in peroxisome biogenesis is currently not clear.
Here, we show that (i) nonfarnesylated and farnesylated human Pex19p display a similar affinity towards a select set of PMPs,
(ii) a variant of Pex19p lacking a functional farnesylation motif is able to restore peroxisome biogenesis in Pex19p-deficient
cells, and (iii) peroxisome protein import is not affected in yeast and mammalian cells defective in one of the enzymes involved
in the farnesylation pathway. Summarized, these observations indicate that the CAAX box-mediated processing steps of Pex19p are dispensable for peroxisome biogenesis in yeast and mammalian cells.
Received 10 March 2006; received after revision 28 April 2006; accepted 30 May 2006 相似文献
158.
Disrupted function and axonal distribution of mutant tyrosyl-tRNA synthetase in dominant intermediate Charcot-Marie-Tooth neuropathy 总被引:6,自引:0,他引:6
Jordanova A Irobi J Thomas FP Van Dijck P Meerschaert K Dewil M Dierick I Jacobs A De Vriendt E Guergueltcheva V Rao CV Tournev I Gondim FA D'Hooghe M Van Gerwen V Callaerts P Van Den Bosch L Timmermans JP Robberecht W Gettemans J Thevelein JM De Jonghe P Kremensky I Timmerman V 《Nature genetics》2006,38(2):197-202
Charcot-Marie-Tooth (CMT) neuropathies are common disorders of the peripheral nervous system caused by demyelination or axonal degeneration, or a combination of both features. We previously assigned the locus for autosomal dominant intermediate CMT neuropathy type C (DI-CMTC) to chromosome 1p34-p35. Here we identify two heterozygous missense mutations (G41R and E196K) and one de novo deletion (153-156delVKQV) in tyrosyl-tRNA synthetase (YARS) in three unrelated families affected with DI-CMTC. Biochemical experiments and genetic complementation in yeast show partial loss of aminoacylation activity of the mutant proteins, and mutations in YARS, or in its yeast ortholog TYS1, reduce yeast growth. YARS localizes to axonal termini in differentiating primary motor neuron and neuroblastoma cultures. This specific distribution is significantly reduced in cells expressing mutant YARS proteins. YARS is the second aminoacyl-tRNA synthetase found to be involved in CMT, thereby linking protein-synthesizing complexes with neurodegeneration. 相似文献
159.
Sniekers M Foulon V Mannaerts GP Van Maldergem L Mandel H Gelb BD Casteels M Van Veldhoven PP 《Cellular and molecular life sciences : CMLS》2006,63(13):1553-1563
The identification of 2-hydroxyphytanoyl-CoA lyase (2-HPCL), a thiamine pyrophosphate (TPP)-dependent peroxisomal enzyme involved
in the α-oxidation of phytanic acid and of 2-hydroxy straight chain fatty acids, pointed towards a role of TPP in these processes.
Until then, TPP had not been implicated in mammalian peroxisomal metabolism. The effect of thiamine deficiency on 2-HPCL and
α-oxidation has not been studied, nor have possible adverse effects of deficient α-oxidation been considered in the pathogenesis
of diseases associated with thiamine shortage, such as thiamine-responsive megaloblastic anemia (TRMA). Experiments with cultured
cells and animal models showed that α-oxidation is controlled by the thiamine status of the cell/tissue/organism, and suggested
that some pathological consequences of thiamine starvation could be related to impaired α-oxidation. Whereas accumulation
of phytanic acid and/or 2-hydroxyfatty acids or their α-oxidation intermediates in TRMA patients given a normal supply of
thiamine is unlikely, this may not be true when malnourished.
Received 23 December 2005; received after revision 10 April 2006; accepted 28 April 2006 相似文献
160.
Biological weapons 总被引:1,自引:0,他引:1
Tegnell A Van Loock F Baka A Wallyn S Hendriks J Werner A Gouvras G 《Cellular and molecular life sciences : CMLS》2006,63(19-20):2223-2228
Adequate public health preparedness for bioterrorism includes the elaboration of an agreed list of biological and chemical agents that might be used in an attack or as threats of deliberate release. In the absence of counterterrorism intelligence information, public health authorities can also base their preparedness on the agents for which the national health structures would be most vulnerable. This article aims to describe a logical method and the characteristics of the variables to be brought in a weighing process to reach a priority list for preparedness. The European Union, in the aftermath of the anthrax events of October 2001 in the United States, set up a task force of experts from multiple member states to elaborate and implement a health security programme. One of the first tasks of this task force was to come up with a list of priority threats. The model, presented here, allows Web-based updates for newly identified agents and for the changes occurring in preventive measures for agents already listed. The same model also allows the identification of priority protection action areas. 相似文献