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651.
652.
Mesodinium rubrum (Lohmann 1908) Jankowski 1976 (= Myrionecta rubra) is a common photosynthetic marine planktonic ciliate which can form coastal red-tides. It may represent a 'species complex' and since Darwin's voyage on the Beagle, it has been of great cytological, physiological and evolutionary interest. It is considered to be functionally a phytoplankter because it was thought to have lost the capacity to feed and possesses a highly modified algal endosymbiont. Whether M. rubrum is the result of a permanent endosymbiosis or a transient association between a ciliate and an alga is controversial. We conducted 'feeding' experiments to determine how exposure to a cryptophyte alga affects M. rubrum. Here we show that although M. rubrum lacks a cytostome (oral cavity), it ingests cryptophytes and steals their organelles, and may not maintain a permanent endosymbiont. M. rubrum does not fall into recognized cellular or functional categories, but may be a chimaera partially supported by organelle robbery. 相似文献
653.
L. Fanuel I. Thamm V. Kostanjevecki B. Samyn B. Joris C. Goffin J. Brannigan J. Van Beeumen J. M. Frère 《Cellular and molecular life sciences : CMLS》1999,55(5):812-818
Two new enzymes which hydrolyse D-alanyl-p-nitroanilide have been detected in Ochrobactrum anthropi LMG7991 extracts. The first enzyme, DmpB, was purified to homogeneity and found to be homologous to the Dap protein produced
by O. anthropi SCRC C1-38 (ATCC49237). The second enzyme, DmpA, exhibits a similar substrate profile when tested on p-nitroanilide derivatives
of glycine and L/D-alanine, but the amounts produced by the Ochrobactrum strain were not sufficient to allow complete purification. Interestingly, the DmpA preparation also exhibited an L-aminopeptidase
activity on the tripeptide L-Ala-Gly-Gly but it was not possible to be certain that the same protein was responsible for both
p-nitroanilide and peptide hydrolysing activities. The gene encoding the DmpA protein was cloned and sequenced. The deduced
protein sequence exhibits varying degrees of similarity with those corresponding to several open reading frames found in the
genomes of other prokaryotic organisms, including Mycobacteria. None of these gene products has been isolated or characterised,
but a tentative relationship can be proposed with the NylC amidase from Flavobacterium sp. K172.
Received 7 December 1998; received after revision 15 March 1999; accepted 22 March 1999 相似文献
654.
Mutations in COL11A2 cause non-syndromic hearing loss (DFNA13) 总被引:13,自引:0,他引:13
McGuirt WT Prasad SD Griffith AJ Kunst HP Green GE Shpargel KB Runge C Huybrechts C Mueller RF Lynch E King MC Brunner HG Cremers CW Takanosu M Li SW Arita M Mayne R Prockop DJ Van Camp G Smith RJ 《Nature genetics》1999,23(4):413-419
We report that mutation of COL11A2 causes deafness previously mapped to the DFNA13 locus on chromosome 6p. We found two families (one American and one Dutch) with autosomal dominant, non-syndromic hearing loss to have mutations in COL11A2 that are predicted to affect the triple-helix domain of the collagen protein. In both families, deafness is non-progressive and predominantly affects middle frequencies. Mice with a targeted disruption of Col11a2 also were shown to have hearing loss. Electron microscopy of the tectorial membrane of these mice revealed loss of organization of the collagen fibrils. Our findings revealed a unique ultrastructural malformation of inner-ear architecture associated with non-syndromic hearing loss, and suggest that tectorial membrane abnormalities may be one aetiology of sensorineural hearing loss primarily affecting the mid-frequencies. 相似文献
655.
656.
Activated T cells regulate bone loss and joint destruction in adjuvant arthritis through osteoprotegerin ligand 总被引:124,自引:0,他引:124
Kong YY Feige U Sarosi I Bolon B Tafuri A Morony S Capparelli C Li J Elliott R McCabe S Wong T Campagnuolo G Moran E Bogoch ER Van G Nguyen LT Ohashi PS Lacey DL Fish E Boyle WJ Penninger JM 《Nature》1999,402(6759):304-309
Bone remodelling and bone loss are controlled by a balance between the tumour necrosis factor family molecule osteoprotegerin ligand (OPGL) and its decoy receptor osteoprotegerin (OPG). In addition, OPGL regulates lymph node organogenesis, lymphocyte development and interactions between T cells and dendritic cells in the immune system. The OPGL receptor, RANK, is expressed on chondrocytes, osteoclast precursors and mature osteoclasts. OPGL expression in T cells is induced by antigen receptor engagement, which suggests that activated T cells may influence bone metabolism through OPGL and RANK. Here we report that activated T cells can directly trigger osteoclastogenesis through OPGL. Systemic activation of T cells in vivo leads to an OPGL-mediated increase in osteoclastogenesis and bone loss. In a T-cell-dependent model of rat adjuvant arthritis characterized by severe joint inflammation, bone and cartilage destruction and crippling, blocking of OPGL through osteoprotegerin treatment at the onset of disease prevents bone and cartilage destruction but not inflammation. These results show that both systemic and local T-cell activation can lead to OPGL production and subsequent bone loss, and they provide a novel paradigm for T cells as regulators of bone physiology. 相似文献
657.
Vincent JL Patel GH Fox MD Snyder AZ Baker JT Van Essen DC Zempel JM Snyder LH Corbetta M Raichle ME 《Nature》2007,447(7140):83-86
The traditional approach to studying brain function is to measure physiological responses to controlled sensory, motor and cognitive paradigms. However, most of the brain's energy consumption is devoted to ongoing metabolic activity not clearly associated with any particular stimulus or behaviour. Functional magnetic resonance imaging studies in humans aimed at understanding this ongoing activity have shown that spontaneous fluctuations of the blood-oxygen-level-dependent signal occur continuously in the resting state. In humans, these fluctuations are temporally coherent within widely distributed cortical systems that recapitulate the functional architecture of responses evoked by experimentally administered tasks. Here, we show that the same phenomenon is present in anaesthetized monkeys even at anaesthetic levels known to induce profound loss of consciousness. We specifically demonstrate coherent spontaneous fluctuations within three well known systems (oculomotor, somatomotor and visual) and the 'default' system, a set of brain regions thought by some to support uniquely human capabilities. Our results indicate that coherent system fluctuations probably reflect an evolutionarily conserved aspect of brain functional organization that transcends levels of consciousness. 相似文献
658.
Flierl MA Rittirsch D Nadeau BA Chen AJ Sarma JV Zetoune FS McGuire SR List RP Day DE Hoesel LM Gao H Van Rooijen N Huber-Lang MS Neubig RR Ward PA 《Nature》2007,449(7163):721-725
It is becoming increasingly clear that the autonomic nervous system and the immune system demonstrate cross-talk during inflammation by means of sympathetic and parasympathetic pathways. We investigated whether phagocytes are capable of de novo production of catecholamines, suggesting an autocrine/paracrine self-regulatory mechanism by catecholamines during inflammation, as has been described for lymphocytes. Here we show that exposure of phagocytes to lipopolysaccharide led to a release of catecholamines and an induction of catecholamine-generating and degrading enzymes, indicating the presence of the complete intracellular machinery for the generation, release and inactivation of catecholamines. To assess the importance of these findings in vivo, we chose two models of acute lung injury. Blockade of alpha2-adrenoreceptors or catecholamine-generating enzymes greatly suppressed lung inflammation, whereas the opposite was the case either for an alpha2-adrenoreceptor agonist or for inhibition of catecholamine-degrading enzymes. We were able to exclude T cells or sympathetic nerve endings as sources of the injury-modulating catecholamines. Our studies identify phagocytes as a new source of catecholamines, which enhance the inflammatory response. 相似文献
659.
660.
Contribution of anthropogenic and natural sources to atmospheric methane variability 总被引:5,自引:0,他引:5
Bousquet P Ciais P Miller JB Dlugokencky EJ Hauglustaine DA Prigent C Van der Werf GR Peylin P Brunke EG Carouge C Langenfelds RL Lathière J Papa F Ramonet M Schmidt M Steele LP Tyler SC White J 《Nature》2006,443(7110):439-443
Methane is an important greenhouse gas, and its atmospheric concentration has nearly tripled since pre-industrial times. The growth rate of atmospheric methane is determined by the balance between surface emissions and photochemical destruction by the hydroxyl radical, the major atmospheric oxidant. Remarkably, this growth rate has decreased markedly since the early 1990s, and the level of methane has remained relatively constant since 1999, leading to a downward revision of its projected influence on global temperatures. Large fluctuations in the growth rate of atmospheric methane are also observed from one year to the next, but their causes remain uncertain. Here we quantify the processes that controlled variations in methane emissions between 1984 and 2003 using an inversion model of atmospheric transport and chemistry. Our results indicate that wetland emissions dominated the inter-annual variability of methane sources, whereas fire emissions played a smaller role, except during the 1997-1998 El Ni?o event. These top-down estimates of changes in wetland and fire emissions are in good agreement with independent estimates based on remote sensing information and biogeochemical models. On longer timescales, our results show that the decrease in atmospheric methane growth during the 1990s was caused by a decline in anthropogenic emissions. Since 1999, however, they indicate that anthropogenic emissions of methane have risen again. The effect of this increase on the growth rate of atmospheric methane has been masked by a coincident decrease in wetland emissions, but atmospheric methane levels may increase in the near future if wetland emissions return to their mean 1990s levels. 相似文献