庆大霉素高产菌株的抗性方法选育

以生产菌株绛红小单孢菌为出发株,应用N 离子注入及紫外线诱变技术,利用高浓度的庆大霉素梯度平板筛选,或配合含有高浓度庆大霉素的液体培养基的生态驯化,筛选到摇瓶效价分别比出发株提高75.2%和70.1%的N08和Wt63两个高产菌株,10 L发酵罐发酵产量分别达2 118 u/mL和1 843 u/mL,C组分含量符合中国药典2000版的规定.     

Benthic foraminiferal fauna turnover at 2.1 Ma in the northern South China Sea

Quantitative analysis of benthic foraminifera from ODP Site 1146 in the northern South China Sea (SCS) shows that abundance of Bulimina alazanensis, sometimes up to about 90%, decreased gradually since 3.2 Ma, especially at 2.1 Ma. Abundance of other benthic foraminiferal species, Globobulimina sub-globosa and Cibicidoides wuellerstorfi, increased after 2.1 Ma. Comparison with changes in oxygen and carbon isotopes of planktonic and benthic foraminifera shows that high abundance values of B. alazanensis corresponded with lower values of oxygen isotope, but for carbon isotope, high values of the species were consistent with heavier carbon isotope of benthic foraminifera and lighter carbon isotope of planktonic foraminifera, respectively, and vice versa. Considering factors such as uplift of Bashi Strait, expansion of the North Hemisphere Glaciation, strengthening of East Asian winter mon-soon and variations in oxygen and carbon isotope of foraminifera, changes of B. alazanensis in ODP Site 1146 suggest that the source of deep water masses of the northern South China Sea changed from the warm Pacific deep water with high oxygen content to Pacific Intermediate water with low oxygen content at 2.1 Ma. In addition, the strengthened East Asian winter monsoon resulted in increased pri-mary productivity, high nutrient and suboxic bottom water. Variations in species of B. alazanensis seemed to be unable to tolerate environmental stress induced by deep water masses and productivity changes.     

Icariin suppresses bone resorption activity of rabbit osteoclasts in vitro

The effect of icariin on the bone resorption activity of rabbit osteoclasts is assessed in vitro. Osteoclasts were isolated from Japanese white rabbits and cultured on plates with a sterilized bone slice in each well. After treatment with icariin at various concentrations, the bone resorption activity of osteoclasts was evaluated by examining pit areas, superoxide anion (·O2-) generation, size and number of actin rings and intracellular calcium concentration [Ca2 ]i. As revealed by these data, icariin elicited continuous decline of [Ca2 ]i, making actin ring constricted and ·O2- generation decreased. These events resulted in smaller and fewer pits which indicate suppressed bone resorption activity of rabbit osteoclasts by icariin.     

Molecular epidemiology and evolution of worldwide enterovirus 71 strains isolated from 1970 to 2004

Human enterovirus 71 （EV71） is one of the major etiological agents of the hand-foot-and-mouth disease （HFMD） that often causes severe neurological complications. Recently, its outbreaks mainly take place in the torrid zone of the Asia-Pacific region. To study the evolution and genetic variability, we collected 532 EV71 strains with almost complete or complete VP1 sequences （891 nt） isolated worldwide from 1970 to 2004. The pairwise homologies and genetic distances were analyzed. Most strains belong to previously identified genotype B and C. However, a unique strain R13223-1ND-01 appears not to fall into current three genotypes （A, B and C）, and probably represents a new genotype D. Some orphan strains were observed in the genotypes B and C, and their significance in the EV71 evolution was discussed. Moreover, there is a significant co-variance of 6 discrete positions on VP1 （amino acid 43, 58, 164, 184, 240 and 249）. This high co-variability is tightly related with the subgenotypes.     

山东省水产品出口现状及对策研究

分析了山东省水产品出口存在的问题,提出了山东省水产品出口的对策。     

Cu（Ⅱ）离子驱动hdpe配体分解构筑的新型超分子配合物（hdpe=2-羟基-2,2′-二吡啶基-乙酮）

在Cu(Ⅱ)离子驱动下,2-羟基-2,2′-二吡啶基-乙酮于甲醇溶液中发生分解形成超分子配合物Cu[(C5H4N)COO]2。通过单晶衍射测定了晶体结构,并用元素分析、红外光谱、热分析等技术对其进行了表征。配合物属单斜晶系,空间群P2(1)/c,a=0.37746(7)nm,b=1.2048(2)nm,c=1.1923(2)nm;α=90°,β=91.598(3)°,γ=90°;Z=2,R=0.0252。配合物中,两个2-羧基吡啶均以二齿螯合方式与Cu(Ⅱ)原子配位,形成具有平面四边形的单核基本单元,相邻单元籍C-H…O氢键的连接构筑了二维层状超分子结构,毗邻的二维结构又通过π-π堆积相互作用拓展为三维超分子体系。     

地铁车站服务标志系统功效综合评价

建立了服务标志系统功效评价指标体系,用层次分析法求出了各评判指标的权重.以上海地铁徐家汇车站为例,用模糊数学理论对其服务标志系统功效进行了综合的评价.概括出今后标志设计中,应该从标志的数量、形式、色彩,标志的醒目性以及信息表达的确切性等方面进一步地完善.     

橙黄色电致发光器件发光特性的研究

本文叙述了橙黄色薄膜电致发光器件(TFEL)和采用陶瓷厚膜作为绝缘层、ZnS:Mn作为发光层的橙黄色陶瓷厚膜电致发光器件(CTFEL).测量了器件的电致发光光谱和亮度-电压曲线,研究了效率-电压等特性.     

大豆蛋白废水对聚砜超滤膜性能影响的研究

从溶液相因素出发，以聚砜超滤膜为研究对象，采用大豆粕溶液为废水液，实验研究溶液相在膜过程中对超滤膜性能的影响．结果表明，膜的污染随着时间的增长越来越严重，不同浓度下在230min时其污染达到平衡，不同pH值在210min时其污染达到平衡，在酸性条件下污染相对严重．     

福建东山湾海浪现场观测的统计特征

为分析福建省南部港湾──东山湾的波浪特征,采用S4型压力式方向波浪仪观测获取现场一个月的风浪过程资料,对其进行了统计分析和频谱分析,给出了波高和周期分布,特征波要素与频谱关系,结果表明:实测波高分布与瑞利分布基本相符;波浪谱的显著部分在狭窄的频率内,对应3、5、6、7级风谱峰密度值(m2s)分别为0.084 3、0.372 8、0.941 8、1.837 7,随着风速的增加,谱能也显著地增加,谱曲线下面的总面积增大,谱的显著部分波及的频率范围也扩大,且谱的显著部分沿低频率的方向推移,对应风浪的波高及周期范围增大.