The effect of daily evening isoproterenol administration on reproductive organ growth in male rats treated neonatally with testosterone propionate

Summary Daily evening injections of isoproterenol extended the period of high pineal N-acetyltransferase activity and retarded the growth of testes, seminal vesicles and prostate in rats treated neonatally with testosterone propionate.The authors are grateful to Mrs Marie Svobodová for her skillful technical assistance.     

Metabolism of the snailCryptozona ligulata during regeneration of optic tentacles

Summary The biochemical changes in the hemolymph and tissues were followed during regeneration of the optic tentacles of the snailCryptozona ligulata (Pulmonata-Stylommatophora). There is a remarkable increase in total carbohydrates in hemolymph and tissues and glycogen in tissues at the expense of free amino acids and fatty acids. It is clear that ablation of the optic tentacles stimulates carbohydrate synthesis through glyconeogenic routes. The optic tentacles regenerate completely in 18–21 days.     

Structure specificity of some immunoadjuvant synthetic glycopeptides

Summary The immunoadjuvant activity of muramyl dipeptide seems to be critically dependent on the type of substitution of the -carboxyl group of the D-isoglutamine residue. Moreover the nonapeptide L-Ala-D-isoGlu-L-Lys-D-Ala-(Gly)₅-OME also shows a definite effect.     

Isoprotenerol induction of pineal serotonin N-acetyltransferase in normotensive and spontaneously hypertensive rats

Zusammenfassung Verabreichung von Isoprotenerol, eines -adrenergen Agonisten, induziert einen signifikant höheren Anstieg der Aktivität der Serotonin-N-Acetyltransferase in der Epiphyse von spontan hypertensiven, im Vergleich mit normotensiven, Rattenmännchen. Derselbe Unterschied wurde auch bei Weibchen beobachtet, jedoch liegen die Aktivitätswerte bei spontan hypertensiven Männchen allgemein höher. Die durch Isoprotenerol erhöhte Induktion der Serotonin-N-Acetyltransferase erzeugt hinsichtlich der Catecholamine in den Pinealozyten von spontan hypertensiven Ratten eine veränderte Reaktivität der Achse -Rezeptor-Adenylzyklasesystem-Serotonin-N-Acetyl transferase.     

The effect of D-6-methyl-8-[β-isopropylaminoethyl] ergoline-I on the lactation in nursing rats

D-6-Methyl-8-[-isopropylaminoethyl] ergoline-I [VÚFB-10726], beginning from the dose of 0.05 mg/kg p.o., suppresses lactation through the inhibition of prolactin secretion in nursing rats.     

Minimum information about a microarray experiment (MIAME)-toward standards for microarray data.

Microarray analysis has become a widely used tool for the generation of gene expression data on a genomic scale. Although many significant results have been derived from microarray studies, one limitation has been the lack of standards for presenting and exchanging such data. Here we present a proposal, the Minimum Information About a Microarray Experiment (MIAME), that describes the minimum information required to ensure that microarray data can be easily interpreted and that results derived from its analysis can be independently verified. The ultimate goal of this work is to establish a standard for recording and reporting microarray-based gene expression data, which will in turn facilitate the establishment of databases and public repositories and enable the development of data analysis tools. With respect to MIAME, we concentrate on defining the content and structure of the necessary information rather than the technical format for capturing it.     

Alternative splicing and genome complexity.

Alternative splicing of mRNA allows many gene products with different functions to be produced from a single coding sequence. It has recently been proposed as a mechanism by which higher-order diversity is generated. Here we show, using large-scale expressed sequence tag (EST) analysis, that among seven different eukaryotes the amount of alternative splicing is comparable, with no large differences between humans and other animals.     

The influence of ethylene on primary attraction of the olive beetle,Phloeotribus scarabaeoides (Bern.) (Col., Scolytidae)

Two experiments have been carried out to verify the effect of ethylene on the primary attraction ofPhloeotribus scarabaeoides under both laboratory and field conditions. The experiments were based on the enhancement of ethylene production by olive branches and trunks after the application of (2-chloroethyl)phosphonic acid. Under laboratory conditions, the beetles' response was strongly stimulated on the treated branches. Under field conditions, the treatment aided the location of the olive wood by the flying beetles, and the attack density was significantly greater in the treated wood at the end of the experiment.