Differences in cardiac myosin light chain LC1 among human, monkey and sheep

The atrial and ventricular myosin light chains of human, monkey and sheep hearts were compared by dodecylsulfate polyacrylamide gel electrophoresis. The atrial light chain 2 and ventricular light chain 2 are similar among these mammals. However, the atrial light chain 1 of monkey has different electrophoretic mobility from those of human and sheep. The monkey ventricular light chain 1 has same mobility as that of sheep but different from that of human.     

Light-dependent antibody labelling of photoreceptors

Monoclonal antibodies are tools widely used to analyse the structure of the nervous system. Whereas some labelling patterns are highly reproducible, others appear to vary from one preparation to the next, as we noticed in particular for some antibodies with respect to photoreceptor labelling. To establish whether some of this variability can be linked to functional criteria, we tested for light-dependence. We found two antibodies that label photoreceptor outer segments, only when the retina has been illuminated, and a third antibody that has a selective affinity for dark-adapted outer segments. The two antibodies against light-activated sites are primarily directed against the highly phosphorylated neurofilament subunit at relative molecular mass 200,000 (200K). One of them, RT97, recognizes on immunoblots, in addition to neurofilaments, a light-activated epitope on a protein that resembles the photopigment rhodopsin, presumably a phosphorylation-dependent site. Antibodies like those described here may allow the study of physiological processes such as light and dark adaptation using morphological techniques.     

Complexed rheumatoid factor measurements in sera, synovial fluids and in immune complex fractions.

Mild acidic treatment increases the rheumatoid factor titre of some sera and synovial fluids (SF) in rheumatoid arthritis (RA), juvenile RA (JRA) and most frequently in rheumatoid vasculitis. This unmasking of 'hidden' RF in serum and SF samples correlated with the RF-immune complexes (RF-IC) and complexed C4 present in the 3% polyethylene glycol (PEG) precipitates, indicating that by means of 'hidden' RF measurements RF-ICs are possibly detected. This method seems to provide a diagnostic tool for detecting RF-ICs in RA and other related diseases.     

Effects of penfluridol of dopamine-sensitive adenylate cyclase in corpus striatum and substantia nigra of rats.

Penfluridol, a neuroleptic with diphenylbutyl piperidine structure, blocked the dopamine-sensitive adenylate cyclase in homogenates of corpus striatum and substantia nigra of rats, probably by a competitive antagonism versus dopamine.     

SO_2两转两吸流程中“ⅢⅠ-ⅣⅡ”和“ⅢⅡ-ⅣⅠ”换热情况浅析

通过工艺计算，定量分析比较 Ｓ Ｏ２ 转化中“ⅢⅠ－ ⅣⅡ”和“ⅢⅡ－ ⅣⅠ”两种换热流程的差异，论证了就换热情况而言，“ⅢⅠ－ ⅣⅡ”流程优于“ⅢⅡ－ ⅣⅠ”流程     

用柱层析和TLC分离小麦PGIP和植保素

植物抗病能力大小的测定需要比较准确可靠且相对简便的办法。采用接种病菌于植物上观察染菌情况虽然直观，但受病菌本身的生理状态及外界环境影响很大，且不便于定量的表述。因此，选择合适的生理生化指标作为植物抗性大小的衡量标准，对于植物病理的研究具有重要的意义。选择了小麦体系的两种抗性物质－ 小麦植保素和 Ｐ Ｇ Ｉ Ｐ作为小麦的抗性指标来分离分析