Escherichia coli swim on the right-hand side

The motion of peritrichously flagellated bacteria close to surfaces is relevant to understanding the early stages of biofilm formation and of pathogenic infection. This motion differs from the random-walk trajectories of cells in free solution. Individual Escherichia coli cells swim in clockwise, circular trajectories near planar glass surfaces. On a semi-solid agar substrate, cells differentiate into an elongated, hyperflagellated phenotype and migrate cooperatively over the surface, a phenomenon called swarming. We have developed a technique for observing isolated E. coli swarmer cells moving on an agar substrate and confined in shallow, oxidized poly(dimethylsiloxane) (PDMS) microchannels. Here we show that cells in these microchannels preferentially 'drive on the right', swimming preferentially along the right wall of the microchannel (viewed from behind the moving cell, with the agar on the bottom). We propose that when cells are confined between two interfaces--one an agar gel and the second PDMS--they swim closer to the agar surface than to the PDMS surface (and for much longer periods of time), leading to the preferential movement on the right of the microchannel. Thus, the choice of materials guides the motion of cells in microchannels.     

Local to unity,long‐horizon forecasting thresholds for model selection in the AR(1)

This article introduces a novel framework for analysing long‐horizon forecasting of the near non‐stationary AR(1) model. Using the local to unity specification of the autoregressive parameter, I derive the asymptotic distributions of long‐horizon forecast errors both for the unrestricted AR(1), estimated using an ordinary least squares (OLS) regression, and for the random walk (RW). I then identify functions, relating local to unity ‘drift’ to forecast horizon, such that OLS and RW forecasts share the same expected square error. OLS forecasts are preferred on one side of these ‘forecasting thresholds’, while RW forecasts are preferred on the other. In addition to explaining the relative performance of forecasts from these two models, these thresholds prove useful in developing model selection criteria that help a forecaster reduce error. Copyright © 2004 John Wiley & Sons, Ltd.     

Drosophila pseudoobscura (Diptera: Drosophilidae) of the Great Basin IV: a release experiment at Bryce Canyon

Some populations of Drosophila pseudoobscura in the Great Basin have very little genetic variation for third chromosome inversion gene arrangements. These populations are essentially monomorphic for the Arrowhead gene arrangement. At Bryce Canyon, Utah, individuals with other gene arrangements (Standard, Pikes Peak, and Treeline) were released and their frequencies monitored. One generation after release, the released arrangements had increased in frequency from 0.7% to almost 10%. After overwintering, the arrangement frequencies were not statistically different from the prerelease samples. The samples did demonstrate a low-level retention of the released Pikes Peak arrangement. The decline in the released arrangements was probably the result of large population size at Bryce Canyon and the bottleneck effects of overwintering. The results do not seem consistent with a model of the released arrangements having a lowered fitness.     

Effects of land clearing on bordering winter annual populations in the Mohave Desert

Normal 0 false false false EN-US X-NONE X-NONE MicrosoftInternetExplorer4 st1\:*{behavior:url(#ieooui) } /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Table Normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-qformat:yes; mso-style-parent:""; mso-padding-alt:0in 5.4pt 0in 5.4pt; mso-para-margin:0in; mso-para-margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:11.0pt; font-family:"Calibri","sans-serif"; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-fareast-font-family:"Times New Roman"; mso-fareast-theme-font:minor-fareast; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin; mso-bidi-font-family:"Times New Roman"; mso-bidi-theme-font:minor-bidi;} Construction of a 10-MWe solar thermal power plant in Daggett, California, involved clearing and leveling 53 ha of desert land. Transient offsite effects of this construction were apparent as a reduced density of annual plants within 100 m downwind of the edge of the cleared area. Schismus arabicus populations recovered within four years, but Erodium cicutarium populations did not. The effects appeared to be related more to an interaction of seed-dispersal mechanisms with the large, open space than to the obvious sand movement and deposition associated with the clearing operations.     

Biomimetic synthesis of cannabispiran

Summary Total synthesis of cannabispiran (1) was accomplished by a biomimetic-type cyclization of the bibenzyl2, using K₃Fe(CN)₆ or MoOCl₄.Acknowledgment. Supported in part by the Research Institute of Pharmaceutical Sciences, School of Pharmacy, University of Mississippi.