Effect of protein molecules on the photoluminescence properties and stability of water-soluble CdSe/ZnS core-shell quantum dots

The fact that the photoluminescence properties of quantum dots are always strongly influenced by the environment limits the scope of further progress in the field of QD’ bio-applications. In this paper, the effects of immunoglobulin G (IgG) on the photo-luminescence properties and stability of water-soluble CdSe/ZnS core-shell quantum dots coated with amphiphilic poly (acrylic acid) (PAA) are studied. Photoluminescence (PL) spectra, UV-vis spectra and excited state lifetime measurements are used to characterize the influence of different protein molecules, such as IgG (goat anti-human IgG, rabbit anti-human IgG, human IgG, and goat anti-human IgG-human IgG conjugates), avidin and bovine serum albumin (BSA) on the PL properties of QDs. The PL intensity and stability of CdSe/ZnS are largely enhanced compared to that of pure CdSe/ZnS QDs when the IgG molecules are added into the QD solution. The PL intensity increases with increasing the IgG concentration, but there appears no influence on the PL peak and a full width at half maximum (FWHM). The PL evolution of QDs as a function of different protein molecules depends on the structure of protein molecules, which is used as a sensor to recognize human IgG. It is inferred that the interaction between PAA coating layer and IgG molecules results in the enhancement of PL intensity. The study of the effect of pH and ion strength on optical properties of QD-IgG mixed solution, compared with the pure QD solution, suggests that pH value and ion strength do not destroy the interaction between the PAA coating layer and IgG. Excited state lifetime analysis indicates that the PL enhancement comes from the passivation of surface of the QDs with the PAA coating layer. IgG molecules have no effects on the properties of the biological system but can increase the stability and PL intensity of CdSe/ZnS QDs, which will enlarge the application of QDs in biomedicine and other fields.     

设计专业教学中创造性思维的培养

从事艺术设计工作一刻也不能离开创意,因而对艺术设计专业的学生要特别注重创意能力的培养;要想让学生具有创意能力,必须培养学生的创造性思维。应该在每一门课程、每一节课的常规教学中把引导与启发学生创造性思维放在核心的位置,通过有针对性的思维训练,帮助学生打破保守思维的枷锁,提升创意能力。     

In-situ cosmogenic 36Cl denudation rates of carbonates in Guizhou karst area

This study quantifies surface denudation of carbonate rocks by the first application of in-situ cosmogenic36Cl in China. Concentrations of natural Cl and in-situ cosmogenic36Cl in bare carbonates from Guizhou karst areas were measured with isotope dilution by accelerator mass spectrometer. The Cl concentration varied from 16 to 206 ppm. The 36Cl concentrations were in range of (0.8-2.4)106 atom g-1 , resulting in total denudation rates of 20-50 mm ka-1 that averaged over a 104-105 a timescale. The 36Cl-denudation rates showed roughly a negative correlation with the local mean temperature. This preliminary observation may suggest the variations of proportions of chemical weathering and physical erosion in denudation process, depending upon local climatic conditions.     

组稿中需要注意和具备的几个问题与能力

组稿是策划编辑的一项重要工作,对选题的落实质量起着重要的影响作用.在组稿过程中需要注意什么问题,以及在组稿工作中需要编辑具备什么能力?本文对此作了简要的论述.     

枯草杆菌JAS株α—淀粉酶的产生与其抗衣霉素的关系

为了研究Bacillus subtilis的产α-淀粉酶性状与其抗衣霉素性状之间的关系,进一步选择在诱变育种时能有效地筛选淀粉酶基因突变株的方法,我们对此作了初步探索.衣霉素敏感型的α-淀粉酶工业生产菌B.subtilis JAS经过紫外线诱变后,选得9株抗衣霉素突变株.通过对它们进行的多次发酵酶活力测定,发现其中3株是α-淀粉酶缺陷型、3株酶活力基本不变或降低,另外3株酶活力明显提高,其中1株酶活力是出发菌株的114%—123%.试验表明B.subtilis α-淀粉酶的产生与其抗衣霉素基因的表达有着密切关系.因此,在对该菌的诱变育种时,可以采用衣霉素为粗筛选择压力.     

用激波管测量固体介质中的空间电荷分布

本文分析了压力阶跃波法测量固体介质中空间电荷分布的基本原理,讨论了激波在气体激波管内的传播及气体激波作用于固体时在固体内引起的波动的过程。用直径为113mm的激波管为主体构成了空间电荷测量系统,并用压力传感器和微型计算机构成了激波波速测量装置,从而较准确地获得了激波波速、激波压力阶跃波形等有关参数。并得到了一些空间电荷的测量结果。     

热处理工艺质量管理的一种模糊数学方法

热处理工艺质量管理是一个复杂多元系统。用模糊方法综合评判模型,全面考虑各工序对产品质量的影响,并依据现场经验、原始统计资料和热处理原理,将这些影响进行数量化处理后,得到 Fuzzy 子集 和。用计算机运算,其结果与实际情况相符。此方法可应用于现场热处理工艺管理。