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191.
Suppressor cell induction can be demonstrated during antigen specific blastogenesis by using the same methods which have shown induction of suppressor cells by Con A. Since suppressor cells are rapidly generated during antigen specific blastogenesis, they must regulate the final level of blastogenesis induced during the seven day in vitro incubation. 相似文献
192.
P. J. Müller P. Masner Maria Kälin W. S. Bowers 《Cellular and molecular life sciences : CMLS》1979,35(5):704-705
Summary Corpora allata fromOncopeltus fasciatus incubated in vitro in medium containing 10–5.35 M (1 g/ml) of precocene II lose their ability to secrete juvenile hormone when reimplanted into last instar larvae.Acknowledgments. We thank Mr K. Dorn, Mrs L. Dolezal, Mrs V. Nötzli-Graf, Mr K.H. Trautmann and Mr A. Schuler for technical help, Dr W. Vogel and Dr A. Dübendorfer for valuable discussions. 相似文献
193.
Summary Viable cerebellar perikarya of mixed cell type obtained from 7–9-day-old rats were maintained in monolayer culture for up to 12 days. During this time extensive neurite formation and outgrowth occurred. The large majority of the cells developing in culture were tentatively identified as granule neurons. This identification was based on the large number isolated from the starting tissue, and the cells general morphological features in culture such as perikaryal and nuclear size, the bipolar nature of neurite extension, and their migratory behaviour.D.N. Currie was supported by a grant from Hoechst Pharmaceuticals, Ld, U.K.J.C. was supported by an S.R.C. Grant to S.P.R. RoseThe authors would like to thank Kathleen Tear for excellent technical assistance and members of the Brain Research Group for helpful discussions. 相似文献
194.
195.
196.
197.
C. P. Sigdestad D. J. Grdina K. Ando 《Cellular and molecular life sciences : CMLS》1979,35(6):815-817
Summary Cells from a murine fibrosarcoma (FSa) have been grown in vitro as multicell tumor spheroids (MTS). The growth rate of these MTS was determined. Follwing selected periods of growth, MTS were made into a single cell suspension and separated on linear density gradients of Renografin. While only 1 population of cells was separated from small spheroids (400 m diameter), at least 3 subpopulations of tumor cells were separated and isolated from large spheroids (800 m in diameter).This investigation was supported in part by grants numbered CA-18628 and CA-06294, awarded by the National Cancer Institute, DHEW. 相似文献
198.
H. Goldman R. Fischer N. Nicolov S. Murphy 《Cellular and molecular life sciences : CMLS》1975,31(3):328-330
Zusammenfassung Die i.v. Verabreichung von LSD an Ratten steigert den Blutkreislauf im Hirnstamm sowie im frontalen und parietalen Cortex selektiv. Es wurden durch 6-Nor-LSD, 2-Methyl-LSD und
8, 9-LSD weder wahrnehmbare Verhaltensänderungen noch Änderungen des regionalen Blutkreislaufs verursacht. 相似文献
199.
Cytological detection of mutagen-carcinogen exposure by sister chromatid exchange. 总被引:40,自引:0,他引:40
A staining technique that detects sister chromatid exchanges (SCEs) has been used to examine the response of chromosomes in cultured Chinese hamster cells to a wide variety of mutagens-carcinogens. The test gives a very sensitive and rapid method for detecting chromosome mutagenicity of chemical agents and provides a powerful new method for detecting environmental mutagens. 相似文献
200.