Design and implementation of a proxy caching system for streaming media

With the widespread use of streaming media application on the Internet, a significant change in Internet workload will be provoked. Caching is one of the applied techniques for enhancing the scalability of streaming system and reducing the workload of server/network. Aiming at the characteristics of broadband network in community, we propose a popularity-based server-proxy caching strategy for streaming medias, and implement the prototype of streaming proxy caching based on this strategy, using RTSP as control protocol and RTP for content transport. This system can play a role in decreasing server load, reducing the traffic from streaming server to proxy, and improving the start-up latency of the client.     

Systematic generation of high-resolution deletion coverage of the Drosophila melanogaster genome

In fruit fly research, chromosomal deletions are indispensable tools for mapping mutations, characterizing alleles and identifying interacting loci. Most widely used deletions were generated by irradiation or chemical mutagenesis. These methods are labor-intensive, generate random breakpoints and result in unwanted secondary mutations that can confound phenotypic analyses. Most of the existing deletions are large, have molecularly undefined endpoints and are maintained in genetically complex stocks. Furthermore, the existence of haplolethal or haplosterile loci makes the recovery of deletions of certain regions exceedingly difficult by traditional methods, resulting in gaps in coverage. Here we describe two methods that address these problems by providing for the systematic isolation of targeted deletions in the D. melanogaster genome. The first strategy used a P element-based technique to generate deletions that closely flank haploinsufficient genes and minimize undeleted regions. This deletion set has increased overall genomic coverage by 5-7%. The second strategy used FLP recombinase and the large array of FRT-bearing insertions described in the accompanying paper to generate 519 isogenic deletions with molecularly defined endpoints. This second deletion collection provides 56% genome coverage so far. The latter methodology enables the generation of small custom deletions with predictable endpoints throughout the genome and should make their isolation a simple and routine task.     

The VER2 promoter contains repeated sequences and requires vernalization for its activity in winter wheat （Triticum aestivum L.）

Previously, we isolated a vernalization-related gene, VER2, from winter wheat (Triticum aestivum L.) and its expression was restricted in the immature leaves of vernalized wheat seedlings. To further investigate the regulation of VER2 expression and the function of its promoter, we isolated a 41.7 kb genomic clone containing VER2 gene from atransformation-competent artificial chromosome (TAC) library of wheat (Triticum aestivum-Haynaldia villosa). The sequence analysis showed that there were eleven predicted genes in the TAC. The exons of gene 3 corresponded to the cDNA sequence of VER2 gene. Analysis of VER2 promoter structure showed that there were three small repeat sequences divided by two large repeat sequences. The putative response elements, such as abscisic acid response elements (ABRE), MeJA-response elements (Me-JARE), low-temperature response elements (LTR), endosperm expression elements, MYB binding sites and similar elements to GA response elements (GARE), were involved in the VER2 promoter region. Construct containing the VER2 promoter (-5895 to 73) driving GFP reporter gene was bombarded into vernalized or non-verualized immature leaves in wheat. The vernalized immature leaves showed bright green fluorescence after incubation for 24 h, however, the green fluorescence was not observed in the non-vernalization leaves under the same condition. These results suggested that vernalization was essential for the function of VER2 promoter in the immature leaves of winter wheat.     

QTL mapping of resistance to sheath blight in maize（Zea mays L.）

Maize sheath blight (Rhizoctonia Solani) is a widely occurring fungus disease with great harm to corn-pro- ducing regions in the world. The first happening of sheath blight in China was reported in Jilin Province as early as in 1966[1]. Since the 1970s, the enlargement of corn- growing regions, the application of maize hybrids, the increasing use of fertilizers, especially the nitrogenous fertilizer, and a higher growth-density, all have caused a quick spread of sheath blight, the occurring …     

Response of global lightning activity to air temperature variation

It is an issue of great attention but yet not very clear whether lightning activities increase or decrease on a warmer world. Reeve et al. presented that lightning activities in global land and the Northern Hemisphere land have positive response to the increase of wet bulb temperature at 1000hPa. Is this positive response restricted only to wet bulb temperature or in land？ What is the response of global lightning activities （in both land and ocean） to the global surface air temperature variation like？ This paper, based on the 5-year or 8-year OTD/LIS satellite-based lightning detecting data and the NCEP reanalysis data, makes a reanalysis of the response of the global and regional lightning activities to temperature variations. The results show that on the interannual time scale the global total flash rate has positive response to the variation in global surface air temperature, with the sensitivity of 17±7% K^-l. Also, the seasonal mean flash rate of continents all over the world and that of continents in the Northern Hemisphere have sensitive positive response to increase of global surface air temperature and wet bulb temperature, with the sensitivity of about 13±5% K^-1, a bit lower than estimation of 40% K^-1 in Reeve et al. However, the Southern Hemisphere and other areas like the tropics show no significant correlation.     

Down-regulation of αGal epitopes by co-transfection of α1,3-galactosidase gene and α1,2–fucosyltransferase gene

The polycarbohydrate structure of Galα1- 3Ga1β1-4GluNAc-R （known as αGal epitopes of xenoantigen）, produced by α1-3-galactosyltransferase （α1,3-GT） in the course of animal development, is the major xenoantigen on the cell surface of porcine which causes hyperacute rejection in pig-to-human xenotransplantation. Alpha-1,3-galactosidase （AGL）, a hydrolytic enzyme, can remove the terminal α1,3-galactosyi from the Galα1-3Galβ1-4GluNAc-R structure resulting in cleaning αGai epitopes from the porcine cells. Aipha-1,2-fucosyitransferase （HT） can modify the surface carbohydrate phenotype of porcine cells, bringing about reduction of αGai epitopes expression. In this study, human AGL and HT gene were co-transfected to porcine fetal fibroblast （PFFb） in equimolar concentration to reduce the xenoantigen. Gene and protein of hAGL and HT were both detected to express at high level by RT-PCR and Western blot, respectively. There was an 84% reduction in αGai xenoantigen and an 82% increase in H antigen as assayed by flow cytometry in the AGL and HT gene co-transfected PFFb. The number and morphology of transgenic PFFb chromosome were normal. Findings indicate that Galα1-3Gal epitopes of PFFb could be down regulated by AGL and HT co-transfection without deleterious effects on the chromosomal profile of the transgenic ceil.     

机器人立体视觉模块的故障诊断

针对机器人的视觉模块，提出了一种故障诊断的算法．该方法根据极线几何理论，利用基本矩阵离线地得到故障诊断的期望范围，再根据匹配像素点是否落在期望范围内判断视觉模块当前是否存在故障．与常规方法相比，不需要增加额外的传感器，且占用系统资源较少，具有相当好的实时性．实验证明，利用该方法进行故障诊断的错判率和漏检率均在3％以下。     

对体育教育专业武术普修课的再认识

随着知识经济和知识社会的到来,教育功能的多元化以及武术发展的需要,作为体育教育专业主干课程的武术已经不再适应新形势的需要。本文主要从武术普修课的教学目标,教材,教学过程,教学评价等诸方面进行重新认识并提出新的要求。同时结合现代化教育理论与思想,探索新的教学模式。     

中西文化差异与英语学习障碍

语言与文化关系密切,语言中的词汇更是与文化息息相关。因此,要学好一门语言就必须深入学习这门语言的文化,了解其民族文化特征。本文从中西文化差异着手,阐明英语学习中的一些障碍。其目的在于首先提高人们对英语语言的认识,以便提高我国英语教学质量。