A method of lipid injection into a fish egg

Zusammenfassung Mittelst Mikropipette von einem Spitzendurchmeser 15–20 werden Lipide in Fischeier injiziert. Die leichtere Löslichkeit der Lipide wird mit Olivenöl oder einem Gemisch Olivenöl/Rizinusöl erreicht. Unmittelbar nach der Befruchtung ist der Einspritzungserfolg am grössten.     

The effect of high phenylalanine concentration on the formation of DOPA from phenylalanine and tyrosine by tyrosine hydroxylase

Zusammenfassung Auf der Basis gegenseitiger Hemmung von Phenylalanin konnte die enzymatische Bildung des DOPA über Phenylalanin und Tyrosin mittels Tyrosinhydroxylase untersucht werden. Eine 70%ige Hemmung der enzymatischen DOPA-Bildung ergab sich im Fall der dreissigfachen Konzentration des Phenylalanin. Die Möglichkeit einer Hemmung der Norepinephrin-Bildung in Phenylketonurie wird diskutiert.     

Effect of thyroid hormone on somatomedin-C release from perfused rat liver

The effect of thyroid hormone on plasma somatomedin-C (SmC) level and on SmC release from perfused rat liver was investigated. Plasma SmC levels and liver tissue SmC were significantly increased in thyroxine-treated rats. Physiological doses of triiodothyronine increased SmC release and SmC concentration in the perfused rat liver. These results indicate that thyroid hormone directly enhances the synthesis and release of SmC in the rat.     

Production of passive cutaneous anaphylaxis (PCA) and reversed PCA by rat IgE antibody in the mouse

Although IgE antibody is generally characterized as a homocytotropic antibody, it has been well known for some time that mouse IgE antibody causes potent sensitization of rat skin for PCA. The present study clearly shows the reciprocal cross-sensitization of mouse skin with rat IgE molecules. PCA and RPCA were produced by rat IgE antibody in an inbred mouse strain. DS/Shi, though not in C3H/HeShi, C57BL/6JShi and BALB/cCrj strains. Sensitization of DS/Shi mouse skin for PCA with rat IgE antibody was comparable in sensitivity with that of rat skin, but lasted only for a short term in comparison with the long persistence in rat skin.     

Critical period for induction of congenital hydrocephalus and dysplasia of subcommissural organ by prenatal X-irradiation in rats

Summary A single whole-body X-irradiation of pregnant Wistar rats at a dose of 1.05 Gy at 10.30, 12.30 and 14.30 h respectively, of gestational day 10 resulted in significantly high incidences of hydrocephalic offspring. No hydrocephalic offspring resulted from X-irradiation of pregnant rats with 1.05 Gy at 16.30 h whereas a dose of 1.22 Gy at 16.30 h resulted in a low but statistically significant incidence of hydrocephalus. Neither 1.05 Gy nor 1.22 Gy X-irradiation of pregnant rats at 18.30 h resulted in any hydrocephalic offspring. Dysplasia of the subcommissural organ was noticed in all the hydrocephalic brains histologically examined.     

Effects of physalaemin,a vase-active peptide from amphibian skin,on the excitability of an identifiable molluscan giant neurone ofAchatina fulica Férussac

Summary We examined effects of several vasoactive peptides (substance P, physalaemin, neurotensin, bradykinin, angiotensin etc.) on the excitability of molluscan giant neurones identified in the subesophageal ganglia ofAchatina fulica Férussac. Of these peptides, only physalaemin showed a remarkable excitatory effect on a giant tonically autoactive neurone.     

Dysplasia of subcommissural organ in congenital hydrocephalus spontaneously occurring in CWS/Idr rats

Summary The subcommissural organ (SCO) of the congenital hydrocephalus spontaneously occurring in CWS/Idr rats was severely reduced in size and displaced at some distance from the anterior end of the cerebral aqueduct. The cerebral aqueduct of the hydrocephalic rats was open throughout its total length during postnatal days 1–20, though it was somewhat narrower at its middle region than in the normal brain.