Mobilization of a transposon in the rice genome

Rice (Oryza sativa L.) is an important crop worldwide and, with the availability of the draft sequence, a useful model for analysing the genome structure of grasses. To practice efficient rice breeding through genetic engineering techniques, it is important to identify the economically important genes in this crop. The use of mobile transposons as gene tags in intact plants is a powerful tool for functional analysis because transposon insertions often inactivate genes. Here we identify an active rice transposon named miniature Ping (mPing) through analysis of the mutability of a slender mutation of the glume-the seed structure that encloses and determines the shape of the grain. The mPing transposon is inserted in the slender glume (slg) mutant allele but not in the wild-type allele. Search of the O. sativa variety Nipponbare genome identified 34 sequences with high nucleotide similarity to mPing, indicating that mPing constitutes a family of transposon elements. Excision of mPing from slg plants results in reversion to a wild-type phenotype. The mobility of the transposon mPing in intact rice plants represents a useful alternative tool for the functional analysis of rice genes.     

Plant recognition of symbiotic bacteria requires two LysM receptor-like kinases

Although most higher plants establish a symbiosis with arbuscular mycorrhizal fungi, symbiotic nitrogen fixation with rhizobia is a salient feature of legumes. Despite this host range difference, mycorrhizal and rhizobial invasion shares a common plant-specified genetic programme controlling the early host interaction. One feature distinguishing legumes is their ability to perceive rhizobial-specific signal molecules. We describe here two LysM-type serine/threonine receptor kinase genes, NFR1 and NFR5, enabling the model legume Lotus japonicus to recognize its bacterial microsymbiont Mesorhizobium loti. The extracellular domains of the two transmembrane kinases resemble LysM domains of peptidoglycan- and chitin-binding proteins, suggesting that they may be involved directly in perception of the rhizobial lipochitin-oligosaccharide signal. We show that NFR1 and NFR5 are required for the earliest physiological and cellular responses to this lipochitin-oligosaccharide signal, and demonstrate their role in the mechanism establishing susceptibility of the legume root for bacterial infection.     

普通成年人在完成运动负荷试验时的心肺功能变化

探讨不同性别各年龄段普通成年人在运动至“力竭”时的心肺功能变化的特点，并就心率、运动时间等指标与运动至“力竭”时吸氧量的关联进行分析．结果表明各项测试指标分布均显示出明显的性别年龄特征；运动时间、心率、工作能力、吸氧量均随年龄的增长而下降；男性在运动时间、工作能力、吸氧量等指标上高于女性．运动至力竭时所需时间与氧量之间显示出高度的相关．     

A scaling law for slow earthquakes

Recently, a series of unusual earthquake phenomena have been discovered, including deep episodic tremor, low-frequency earthquakes, very-low-frequency earthquakes, slow slip events and silent earthquakes. Each of these has been demonstrated to arise from shear slip, just as do regular earthquakes, but with longer characteristic durations and radiating much less seismic energy. Here we show that these slow events follow a simple, unified scaling relationship that clearly differentiates their behaviour from that of regular earthquakes. We find that their seismic moment is proportional to the characteristic duration and their moment rate function is constant, with a spectral high-frequency decay of f(-1). This scaling and spectral behaviour demonstrates that they can be thought of as different manifestations of the same phenomena and that they comprise a new earthquake category. The observed scale dependence of rupture velocity for these events can be explained by either a constant low-stress drop model or a diffusional constant-slip model. This new scaling law unifies a diverse class of slow seismic events and may lead to a better understanding of the plate subduction process and large earthquake generation.     

TRIM25 RING-finger E3 ubiquitin ligase is essential for RIG-I-mediated antiviral activity

Retinoic-acid-inducible gene-I (RIG-I; also called DDX58) is a cytosolic viral RNA receptor that interacts with MAVS (also called VISA, IPS-1 or Cardif) to induce type I interferon-mediated host protective innate immunity against viral infection. Furthermore, members of the tripartite motif (TRIM) protein family, which contain a cluster of a RING-finger domain, a B box/coiled-coil domain and a SPRY domain, are involved in various cellular processes, including cell proliferation and antiviral activity. Here we report that the amino-terminal caspase recruitment domains (CARDs) of RIG-I undergo robust ubiquitination induced by TRIM25 in mammalian cells. The carboxy-terminal SPRY domain of TRIM25 interacts with the N-terminal CARDs of RIG-I; this interaction effectively delivers the Lys 63-linked ubiquitin moiety to the N-terminal CARDs of RIG-I, resulting in a marked increase in RIG-I downstream signalling activity. The Lys 172 residue of RIG-I is critical for efficient TRIM25-mediated ubiquitination and for MAVS binding, as well as the ability of RIG-I to induce antiviral signal transduction. Furthermore, gene targeting demonstrates that TRIM25 is essential not only for RIG-I ubiquitination but also for RIG-I-mediated interferon- production and antiviral activity in response to RNA virus infection. Thus, we demonstrate that TRIM25 E3 ubiquitin ligase induces the Lys 63-linked ubiquitination of RIG-I, which is crucial for the cytosolic RIG-I signalling pathway to elicit host antiviral innate immunity.     

DMY is a Y-specific DM-domain gene required for male development in the medaka fish

Although the sex-determining gene Sry has been identified in mammals, no comparable genes have been found in non-mammalian vertebrates. Here, we used recombinant breakpoint analysis to restrict the sex-determining region in medaka fish (Oryzias latipes) to a 530-kilobase (kb) stretch of the Y chromosome. Deletion analysis of the Y chromosome of a congenic XY female further shortened the region to 250 kb. Shotgun sequencing of this region predicted 27 genes. Three of these genes were expressed during sexual differentiation. However, only the DM-related PG17 was Y specific; we thus named it DMY. Two naturally occurring mutations establish DMY's critical role in male development. The first heritable mutant--a single insertion in exon 3 and the subsequent truncation of DMY--resulted in all XY female offspring. Similarly, the second XY mutant female showed reduced DMY expression with a high proportion of XY female offspring. During normal development, DMY is expressed only in somatic cells of XY gonads. These findings strongly suggest that the sex-specific DMY is required for testicular development and is a prime candidate for the medaka sex-determining gene.     

Low-frequency earthquakes in Shikoku, Japan, and their relationship to episodic tremor and slip

Non-volcanic seismic tremor was discovered in the Nankai trough subduction zone in southwest Japan and subsequently identified in the Cascadia subduction zone. In both locations, tremor is observed to coincide temporally with large, slow slip events on the plate interface downdip of the seismogenic zone. The relationship between tremor and aseismic slip remains uncertain, however, largely owing to difficulty in constraining the source depth of tremor. In southwest Japan, a high quality borehole seismic network allows identification of coherent S-wave (and sometimes P-wave) arrivals within the tremor, whose sources are classified as low-frequency earthquakes. As low-frequency earthquakes comprise at least a portion of tremor, understanding their mechanism is critical to understanding tremor as a whole. Here, we provide strong evidence that these earthquakes occur on the plate interface, coincident with the inferred zone of slow slip. The locations and characteristics of these events suggest that they are generated by shear slip during otherwise aseismic transients, rather than by fluid flow. High pore-fluid pressure in the immediate vicinity, as implied by our estimates of seismic P- and S-wave speeds, may act to promote this transient mode of failure. Low-frequency earthquakes could potentially contribute to seismic hazard forecasting by providing a new means to monitor slow slip at depth.     

Superflares on solar-type stars

Solar flares are caused by the sudden release of magnetic energy stored near sunspots. They release 10(29) to 10(32)?ergs of energy on a timescale of hours. Similar flares have been observed on many stars, with larger 'superflares' seen on a variety of stars, some of which are rapidly rotating and some of which are of ordinary solar type. The small number of superflares observed on solar-type stars has hitherto precluded a detailed study of them. Here we report observations of 365 superflares, including some from slowly rotating solar-type stars, from about 83,000 stars observed over 120 days. Quasi-periodic brightness modulations observed in the solar-type stars suggest that they have much larger starspots than does the Sun. The maximum energy of the flare is not correlated with the stellar rotation period, but the data suggest that superflares occur more frequently on rapidly rotating stars. It has been proposed that hot Jupiters may be important in the generation of superflares on solar-type stars, but none have been discovered around the stars that we have studied, indicating that hot Jupiters associated with superflares are rare.     

教育环境与儿童空间认知的城镇空间组成研究

文中从发展对儿童适宜的生活环境的观点出发来研究城市住房项目．该项研究是针对Makahari湾城镇的中层住宅及附属教育设施的公寓区展开的．文中采用草图来确定Makahari湾城镇中儿童生活与其环境之间的关系．1999年进行首次研究，接着在2003年进行第二次研究，此间该镇的建设有所发展．具体研究是利用儿童凭记忆点出的关于他们的住宅、学校、玩耍场地和其它较多被注意的场所的草图来进行的．在两次研究期间，该地区发生了许多实体环境的变化，这些变化能从儿童的草图的许多元素中反映出来．将这些草图中的变化加以对照、分析，并将环境的改变与儿童的空间认知的关系加以探讨，同时注意分析比较体现在草图中的区域范围。结果表明，儿童早期对目标物的了解能从Makahari湾的空间结构以及环境变化中得到增强，而两者都由方格的模式构成．经验、每目的生活以及对小学和儿童游戏场所的空间认知与儿童的生活环境息息相关．此项分析揭示了在一个发展中的住宅区中儿童对其生活环境的空间认知与其附属教育设施之间的关系．     

Determinant solutions of the nonautonomous discrete Toda equation associated with the deautonomized discrete KP hierarchy

##正## It is shown that the nonautonomous discrete Toda equation and its Backlund transformationcan be derived from the reduction of the hierarchy of the discrete KP equation and the discrete two-dimensionalToda equation.Some explicit examples of the determinant solutions of the nonautonomousdiscrete Toda equation including the Askey-Wilson polynomial are presented.Finally we discuss therelationship between the nonautonomous discrete Toda system and the nonautonomous discrete Lotka-Volterraequation.