Affinity chromatography of monoamine oxidase

Summary A method is described for the purification of pig liver monoamine oxidase by affinity chromatography, using a colum with covalently bound pargyline.Acknowledgment. The authors wish to thank the University Research Council for financial support.     

Human cdc2 protein kinase is a major cell-cycle regulated tyrosine kinase substrate

cdc2 is a catalytic subunit of a protein kinase complex, called the M-phase promoting factor, that induces entry into mitosis and is universal among eukaryotes. In HeLa cells, cdc2 is shown to be the most abundant phosphotyrosine-containing protein and its phosphotyrosine content is subject to cell-cycle regulation. One site of cdc2 tyrosine phosphorylation in vivo is selectively phosphorylated by pp60c-src in vitro.