Sequencing of phosphopeptides sulfonated by 4-sulfophenyl isothiocyanate using post-source decay matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

Phosphorylation/dephosphorylation is probably the most common and important reversible post-translational modification of proteins. Analyzing the functional effects of phosphorylation is helpful for understanding the biological functions of proteins. Identification of the phosphorylation sites of phosphorylated protein is a prerequisite for research on phosphorylation. In this work, an effective and simple method of identification of protein phosphorylation sites has been developed. Phosphopeptides were selectively enriched with immobilized metal affinity chromatography (IMAC) and subsequently chemically modified by 4-sulfophenyl isothiocyanate, and then the chemically modified phosphopeptides were sequenced with post-source decay (PSD) matrix-assisted laser desorption/ionization (MALDI) time-of-flight mass spectrometry for detecting phosphorylation sites. The charge of derivatization by 4-sulfophenyl isothiocyanate introduces a negative sulfonic acid group at the N-terminus of a peptide, and enables the selective detection of only a single series of C-terminal y-type ions. This chemically assisted method greatly simplifies the extremely complex pattern of PSD fragment ions and makes the PSD spectra more easier to be interpreted. The phosphorylation sites of a synthesized model phosphopeptide and human c-myc protein have been successfully identified by this method.     

Discovery of the budding phosphatized globular fossils from the Neoproterozoic Doushantuo Formation at Weng’an, Guizhou Province, China

There is much debate on biological affinities of the phosphatized globular fossils from the Neoproterozoic Doushantuo phosphorites at Weng’an, Guizhou Province. Here we report for the first time the budding structures of some of these globular fossils, which have previously been interpreted as the resting eggs and early cleavage embryos of metazoans. The budding structures are similar to the germinating tubes of the spore and zygote of living algae, suggesting that some globular fossils from the Doushantuo phosphorites bear affinity with algae. The present new information indicated various biological affinity of the phosphatized globular fossils from the Doushantuo phosphorites.     

fMRI time series analysis based on stationary wavelet and spectrum analysis

The low signal to noise ratio (SNR) of functional MRI (fMRI) prefers more sensitive data analysis methods. Based on stationary wavelet transform and spectrum analysis, a new method with high detective sensitivity was developed for analyzing fMRI time series, which does not require any prior assumption of the characteristics of noises. In the proposed method, every component of fMRI time series in the different time-frequency scales of stationary wavelet transform was discerned by the spectrum analysis, then the components from noises were removed using the stationary wavelet transform, finally the components of real brain activation were detected by cross-correlation analysis. The results obtained from both simulated and in vivo visual experiments illustrated that the proposed method has much higher sensitivity than the traditional cross-correlation method.     

基于SIP的上下文感知业务装置

随着下一代网络（NGN）各种技术的遥步发展，如何在NGN中使用户不受干扰地享受各种业务将成为未来应用的热点，目前研究的上下史感知（context—aware）技术正好可以实现这个目的，因此，设计一种协议来实现各种基于context的自适应业务是至关重要的。考虑到SIP协议灵活且易于扩展，结合当前一些广泛应用的context—awsre业务，提出了一个基于SIP扩展的CASIP。通过对context的研究，引入context服务器，给出了增强的网络架构，详细阐述了基于CASIP协议的上下文感知业务的实现技术。     

硕士研究生招生考试制度的研究与设想

为了提高硕士研究生的招生质量,优化招生考试过程,新的硕士研究生招生考试模式应该包括“2 X”研究生入学资格考试、科学的研究生入学报名、研究生入学专业考试和新型录取标准。     

本科教学工作水平评估网站的建设

剖析了我校本科教学工作水平评估网站的建设,采用Macromed ia DreamweaverMX 2004为主要开发软件,结合FireworkMX,FlashMX及java,asp等脚本语言,后台利用微软的中小型数据库Access,实现网页的静态及动态设计与管理工作。     

Removal of disinfection by-product formation potentials by biologically assisted GAC treatment

The object of this paper is to evaluate the removal of disinfection by-products formation potential by artificially intensified biological activated carbon（BAC） process which is developed on the basis of traditional ozone granular activated carbon （GAC）. The results show that 23.1% of trihalomethane formation potential （THMFP） and 68% of haloacetic acid formation potential （HAAFP） can be removed by BAC, respectively. Under the same conditions, the removal rates of the same substances were 12.2% and 13-25% respectively only by GAC process. Compared with GAC, the high removal rates of the two formed potential substances were due to the increasing of bioactivity of the media and the synergistic capabilities of biological degradation cooperating with activated carbon adsorption of organic compounds. BAC process has some advantages such as long backwashing cycle time, low backwashing intensity and prolonged activated carbon lifetime, etc.     

知识型企业知识状态系统演变的PSO机理及其优化模型分析

在知识工作者个人知识挖掘与整个知识型企业知识目标的协同的基础上,知识型企业与知识工作者之间挖掘知识集,不断进行协调从而推动知识状态系统演变的过程与鸟群或鱼群捕食过程的社会行为相似。利用PSO信息处理及优化机理,分析知识型企业知识状态系统演变的知识挖掘、传递以及决策特征,研究知识型企业知识状态系统的PSO模式,为进一步研究知识型企业知识状态系统演变特点的管理理论奠定基础。     

企业知识结构及其优化机制

主要研究了企业知识结构的内涵和优化方法,提出了知识结构的优化机制,认为从人才结构方面来优化企业知识结构,主要任务是明晰人才结构和知识结构的对应关系,促使企业人才结构不断去适应企业知识结构;从知识使用成本方面优化知识结构,就是要积极吸收和创造能够带来利润的知识,同时把那些不能给企业带来利润的知识外化;从企业知识转化方面优化企业知识结构,就是要加速企业知识的正向转化,阻止和延缓企业知识的逆向转化,促进企业核心知识内部的转化。     

面向新农村建设的农业社会化服务体系

农业社会化服务体系是事关社会主义新农村建设的全局性、关键性的基础工程之一。新的形势下,农业社会化服务体系定位不准确、结构不合理、功能不完善已经成为影响新农村建设的重大现实问题。进一步加强农业社会化服务工作,健全完善农村经营服务体系、公益性农业技术推广体系和农业执法服务体系建设,对促进农村经济发展,实现农业增效、农民增收和农村发展,意义重大而且紧迫重要。运用系统分析方法,综合考虑新农村服务体系的构成要素,着重从“服务组织系统”和“服务创新系统”的角度,阐述面向新农村建设的社会化服务体系的构建。