全文获取类型
收费全文 | 11710篇 |
免费 | 32篇 |
国内免费 | 54篇 |
专业分类
系统科学 | 39篇 |
丛书文集 | 64篇 |
教育与普及 | 31篇 |
理论与方法论 | 31篇 |
现状及发展 | 4779篇 |
研究方法 | 558篇 |
综合类 | 6122篇 |
自然研究 | 172篇 |
出版年
2012年 | 171篇 |
2011年 | 347篇 |
2010年 | 67篇 |
2009年 | 63篇 |
2008年 | 197篇 |
2007年 | 244篇 |
2006年 | 227篇 |
2005年 | 275篇 |
2004年 | 280篇 |
2003年 | 223篇 |
2002年 | 228篇 |
2001年 | 471篇 |
2000年 | 481篇 |
1999年 | 338篇 |
1994年 | 286篇 |
1992年 | 277篇 |
1991年 | 219篇 |
1990年 | 261篇 |
1989年 | 223篇 |
1988年 | 216篇 |
1987年 | 215篇 |
1986年 | 223篇 |
1985年 | 299篇 |
1984年 | 225篇 |
1983年 | 180篇 |
1982年 | 155篇 |
1981年 | 158篇 |
1980年 | 170篇 |
1979年 | 379篇 |
1978年 | 286篇 |
1977年 | 249篇 |
1976年 | 218篇 |
1975年 | 222篇 |
1974年 | 242篇 |
1973年 | 217篇 |
1972年 | 249篇 |
1971年 | 286篇 |
1970年 | 340篇 |
1969年 | 265篇 |
1968年 | 268篇 |
1967年 | 237篇 |
1966年 | 251篇 |
1965年 | 168篇 |
1959年 | 89篇 |
1958年 | 139篇 |
1957年 | 98篇 |
1956年 | 70篇 |
1955年 | 64篇 |
1954年 | 67篇 |
1948年 | 64篇 |
排序方式: 共有10000条查询结果,搜索用时 281 毫秒
41.
Summary Calcium is released from the isolated heavy sarcoplasmic reticulum (SR) of frog skeletal muscle upon application of 0.1–1 mM diethylpyrocarbonate (DEP, an imidazolyl reagent). The Ca-ATPase activity of SR was suppressed by 20% in the presence of 1 mM DEP. More than 1 mM of free magnesium ion or 5 M ruthenium red eliminated the effect of DEP on calcium release but not on Ca-ATPase activity. A plausible site of DEP action is on the calcium channel. 相似文献
42.
T. Hatano Y. Kato M. Katayama S. Marumo 《Cellular and molecular life sciences : CMLS》1989,45(4):400-402
Summary A new potent antiauxin, -(5,7-dichloroindole-3-)isobutyric acid has been synthesized and shown to inhibit auxin-mediated elongation ofAvena coleoptiles and to stimulate root growth of rice seedlings. Its activity is stronger than -(p-chlorophenoxy)isobutyric acid and is comparable to that of 2,3,5-triiodobenzoic acid, which are typical antiauxins. 相似文献
43.
H. Nawrath J. Rupp H. Jakob U. Sack F. Mertzlufft W. Dick 《Cellular and molecular life sciences : CMLS》1989,45(4):337-339
Summary The opioid agonists morphine (selective for -receptors) and ethylketocyclazocine (selective for kappa-receptors), at concentrations evoking strong effects in neuronal structures, did not significantly affect the configuration of the intracellularly recorded action potential and the force of contraction in ventricular heart muscle isolated from guinea pigs, rabbits and man. These results suggest that any changes of heart functions in vivo in response to opioid-like drugs are probably not mediated postsynaptically at the myocardial cell membrane but rather presynaptically, influencing the release of noradrenaline and/or acetylcholine from the nerve terminals. 相似文献
44.
Summary Apart from cancer and mutation induction, radiobiological effects on mammals are mostly attributable to cell death, defined as loss of proliferative capacity. Survival curves relate retention of that capacity to radiation dose, and often manifest a quasi-threshold (shoulder). The shoulder is attributable to an initial mechanism of repair (Q-repair) which is gradually depleted as dose increases. Another form of repair, which is not depleted (P-repair), increases the dose required to deliver an average of one lethal event per cell (dose D0). Neither form of repair can unambiguously be linked with repair of defects in isolated DNA. An important initial lesion may well be disruption of the complex structural relationship between the DNA, nuclear membrane and associated proteins. One form of P-repair may be restoration of that structural relationship. 相似文献
45.
B. Lindblad W. E. Burkel T. W. Wakefield L. M. Graham J. C. Stanley 《Cellular and molecular life sciences : CMLS》1988,44(3):223-224
Summary The most important effect of dihydroergotamine is venoconstriction, but certain metabolic effects and changes in vessel prostanoid activity have also been suggested. In this study endothelial cell production of 6-keto PGF1 and TxB2 was quantitated in vitro. No evidence of altered prostanoid production was noted after incubation with dihydroergotamine (exposure ranging from 5×10–3 to 5×10–7 g/l). Similarly, no effect of dihydroergotamine on the growth rates of endothelial cells or smooth muscle cells in vitro was documented. 相似文献
46.
E. Flückiger U. Briner B. Clark A. Closse A. Enz P. Gull A. Hofmann R. Markstein L. Tolcsvai H. R. Wagner 《Cellular and molecular life sciences : CMLS》1988,44(5):431-436
Summary The profile of action in animals of CQP 201-403, a novel 8-amino-ergoline, is in most aspects that of a very potent dopaminomimetic, both as a prolactin secretion inhibitor, and at the levels of the CNS and the cardiovascular system. Qualitatively CQP 201-403 differs slightly from bromocriptine and apomorphine in its effects on the CNS (no influence on serotonin metabolism in the rat cortex; induction of masculine mounting behavior in rats) and the cardiovascular system of the dog (reflex tachycardia in response to a blood-pressure fall). In man the new compound proved to be highly active in lowering prolactin serum levels and to be more potent than bromocriptine (Parlodel®).In memory of Dr Annemarie Closse, who died 14 June 1987. 相似文献
47.
Free R value: a novel statistical quantity for assessing the accuracy of crystal structures 总被引:32,自引:0,他引:32
Brünger AT 《Nature》1992,355(6359):472-475
The determination of macromolecular structure by crystallography involves fitting atomic models to the observed diffraction data. The traditional measure of the quality of this fit, and presumably the accuracy of the model, is the R value. Despite stereochemical restraints, it is possible to overfit or 'misfit' the diffraction data: an incorrect model can be refined to fairly good R values as several recent examples have shown. Here I propose a reliable and unbiased indicator of the accuracy of such models. By analogy with the cross-validation method of testing statistical models I define a statistical quantity (R(free) (T) that measures the agreement between observed and computed structure factor amplitudes for a 'test' set of reflections that is omitted in the modelling and refinement process. As examples show, there is a high correlation between R(free) (T) and the accuracy of the atomic model phases. This is useful because experimental phase information is usually inaccurate, incomplete or unavailable. I expect that R(free) (T) will provide a measure of the information content of recently proposed models of thermal motion and disorder, time-averaging and bulk solvent. 相似文献
48.
M. T. Santini R. Masella A. Cantafora S. W. Peterson 《Cellular and molecular life sciences : CMLS》1992,48(1):36-39
We have recently demonstrated, using electron paramagnetic resonance (EPR) spectroscopy, that insulin receptor internalization in response to insulin incubation (down-regulation) in human erythrocytes is accompanied by a transient decrease in membrane order, as measured by the 2T order parameter. Since membrane lipids play such an important role in receptor internalization, we investigated the possible effects that an alteration of the normally-occurring lipid profile might have on down-regulation and the concomitant transient decrease in membrane order. Consequently, human erythrocytes enriched with cholesterol and erythrocytes from cirrhotic patients were examined, because both of these groups of cells have a higher cholesterol/phospholipid molar ratio (CH/PL) than controls. The 5-nitroxystearate spin label, which inserts into the lipid bilayer of cell membranes, was used to monitor changes in 2T for a 3-h period at 37°C. We report here that both cholesterol-enriched and cirrhotic erythrocytes do not down-regulate, as demonstrated by binding assays, and that they do not show the typical transient decrease in membrane order observed in controls. The results seem to indicate that a more ordered membrane inhibits internalization of the insulin receptor in erythrocytes, and that an increase in membrane disorder is necessary for insulin receptor down-regulation. 相似文献
49.
D. Porquet M. Appel T. Fournier O. Bertaux D. Biou J. Féger 《Cellular and molecular life sciences : CMLS》1992,48(3):257-261
Both in vivo and in vitro models have certain disadvantages for the study of the chronic hepatotoxicity of drugs. The aim of this work was to evaluate a new approach based on an in vivo/in vitro model. After chronic in vivo treatment of rats with Vincamine and Vindeburnol (an eburnamenine derivative which exhibits hepatotoxic properties in man) liver cells were isolated, and functional and metabolic disorders (metabolic utilization of fructose and protein biosynthesis) were studied to determine injury. The results showed no modification of blood parameters, but a direct relationship between the dose of Vindeburnol administered in vivo and the metabolic disorders observed in vitro, evidencing the high sensitivity and reliability of this model. 相似文献
50.
R. Wiesendanger B. Martinoni T. Boller D. Arigoni 《Cellular and molecular life sciences : CMLS》1986,42(2):207-209
Summary Under the action of the appropriate synthase from ripe tomatoes a 11 mixture of (3S, 4R)-[3,4-2H2] and (3R, 4S)-[3,4-2H2]-(2S)-adenosylmethionine is transformed into a 11 mixture of the two meso forms of [2H2]-1-aminocyclopropanecarboxylic acid, a result which proves the operation of an inversion mechanism and which is consistent with direct nucleophilic displacement of the leaving group in the substrate. 相似文献