Radiation induced avoidance behavior transfer by brain extracts of mice

Zusammenfassung In «naiven» Mäusen werden Verhaltensveränderungen nach Injektion kleiner Mengen von Gehirnmaterial aus Saccharin bevorzugenden und röntgenbestrahlten Mäusen beobachtet. Es trat Vermeidung der Saccharinlösung ein, und zwar ähnlich wie sonst in «trainierten», Saccharin bevorzugenden Tieren nach der Bestrahlung.     

Inhibition of neurotensin (NT)-induced glucagon release by [D-Trp11]-NT1

Summary A synthetic analog of neurotensin (NT), [D-Trp¹¹]-NT, antagonized NT-induced hyperglucagonemia and hyperglycemia, and also NT-induced glucagon release from pancreatic islets in rats.This work was supported in part by a grant from the Imanaga Medical Research Foundation, Nagoya, Japan. Address for reprint requests: M.U., Department of Surgery, National Nagoya Railway Hospital, Taiko 1-chome, Nakamura-ku, Nagoya 453, Japan.     

Cytotoxicity of new cytochalasans fromChaetomium globosum

Zusammenfassung Nachweis, dass CHCl₃-Rohextrakte aus Myzelien und Filtraten der Zuchtmedien vonChaetomium globosum Kunze ex Fries einen akuten toxischen Effect auf HeLa-Zellen in vitro, sowie auf Mäuse zeigten. Chaetoglobosine die aus Mycelium-Rohextrakt isoliert wurden und chemisch zu den Cytochalasanen gehören, induzierten in HeLa-Zellen eine Polynukleierung und multipolare Kernverteilung. Auch beim Chaetogobosin wird eine Mikrofilament-Schädigung angenommen.     

Purification of a protein binding quinacrine and histrionicotoxin from membrane fragments rich in cholinergic receptors in Torpedo marmorata]

A protein is purified by differential centrifugation from membrane fragments rich in acetylcholine receptor prepared from Torpedo marmorata electric organ after dissolution by a mixture of non denaturing detergents. After polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulphate and Coomassie blue staining the purified protein yields a single band of apparent molecular weight 43,000. Spectroscopic experiments carried out in the absence of Ca++ and detergents reveal that the 43 K protein interacts with the fluorescent local anesthetic quinacrine and with the frog toxin histrionicotoxin (apparent KD : 7 X 10(-7) M) but not with carbamylcholine and the alpha toxin from N. nigricollis.     

Effect of orotic acid on liver glycogen of different animal species

Summary The effect of orotic acid on the liver glycogen content in the mice, frogs and catfish was studied. It was observed that the orotic acid significantly increases the glycogen content in the liver of mice and catfish as it does in rats. On the other hand it causes a fall of the glycogen level in frogs in experiments made both in autumn and spring. This effect was modified by amino acids administrated together with orotic acid.