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951.
Characterization and molecular cloning of a bovine lentivirus related to human immunodeficiency virus 总被引:8,自引:0,他引:8
M A Gonda M J Braun S G Carter T A Kost J W Bess L O Arthur M J Van der Maaten 《Nature》1987,330(6146):388-391
952.
Early experience of tactile stimulation influences organization of somatic sensory cortex 总被引:5,自引:0,他引:5
Visual experience is essential for the establishment of the cerebral cortical circuitry that allows normal binocular vision. For example, the pattern of right-eye, left-eye dominance columns is permanently altered by simply closing an eye of a young primate. A critical issue is whether environmental factors also influence the development of other cortical sensory areas. In the present experiments we manipulated the tactile experience of young rats by depriving them of the sensory information that is normally provided by their large facial whiskers. Electrophysiological analyses showed that simply trimming the whiskers from the day of birth results in pronounced abnormalities in the response properties of single neurons in the adult somatic sensory cortex. Thus functional plasticity in response to early experience appears to be a fundamental aspect of cortical development. 相似文献
953.
Kinetics of smooth and skeletal muscle activation by laser pulse photolysis of caged inositol 1,4,5-trisphosphate 总被引:1,自引:0,他引:1
Inositol 1,4,5-trisphosphate (InsP3) can stimulate skinned smooth and skeletal muscle to contract by initiating Ca2+ release from the sarcoplasmic reticulum. Whether this process is an integral component of the in vivo muscle activation mechanism was tested by releasing InsP3 rapidly within skinned muscle fibers of rabbit main pulmonary artery and frog semitendinosus. InsP3 was liberated on laser pulse photolysis of a photolabile but biologically inactive precursor of InsP3 termed caged InsP3. Caged InsP3 is a mixture of compounds in which InsP3 is esterified with 1(2-nitrophenyl)diazoethane (probably at the P4- or P5-position). Photochemical release of InsP3 induced a full contraction in both muscles at physiological free Mg2+ concentrations, but only in the smooth muscle were the InsP3 concentration (0.5 microM) and the activation rate compatible with the in vivo physiological response. Endogenous InsP3-specific phosphatase activity was present in smooth muscle and had about 35-fold greater activity than that in the skeletal-muscle preparation. Caged InsP3 was not susceptible to phosphatases in either preparation. 相似文献
954.
955.
956.
Microtubules are versatile cellular polymers that play a role in cell shape determination and mediate various motile processes such as ciliary and flagellar bending, chromosome movements and organelle transport. That a sliding microtubule mechanism can generate force has been demonstrated in highly ordered structures such as axonemes, and microtubule-based force generation almost certainly contributes to the function of mitotic and meiotic spindles. Most cytoplasmic microtubule arrays, however, do not exhibit the structural regularity of axonemes and some spindles, and often appear disorganized. Yet many cellular activities (such as shape changes during morphogenesis, axonal extension and spindle assembly) involve highly coordinated microtubule behaviour and possibly require force generated by an intermicrotubule sliding mechanism, or perhaps use sliding to move microtubules rapidly into a protrusion for stabilization. Here we show that active sliding between cytoplasmic microtubules can occur in microtubule bundles of the amoeba Reticulomyxa. A force-producing mechanism of this sort could be used by this organism to facilitate the extension of cell processes and to generate the dynamic movements of the cytoplasmic network. 相似文献
957.
958.
Leers MP Björklund V Björklund B Jörnvall H Nap M 《Cellular and molecular life sciences : CMLS》2002,59(8):1358-1365
We investigated the distribution and fate of apoptotic bodies during human development and in the adult, using an antibody
(M30) that recognizes a neo-epitope formed early in the apoptotic cascade by caspase cleavage of cytokeratin 18. In the fetus,
we found extensive accumulation of M30-positive, non-phagocytosed fragments in the red pulp of the spleen, subcutaneous and
submucosal vessels, the interstitium of the lung, and the glomerular mesangium of the kidneys. In the liver, M30-immunoreactive
fragments were found inside macrophages in the sinusoids. The number of these fragments and the intensity of the immunostaining
increased with the gestational age of the fetus. In the adult, M30-positive fragments were barely detectable in normal tissues.
However, many pathological situations, including both chronic degenerative processes and metastatic cancer, were associated
with accumulation of M30-positive fragments in the red pulp of the spleen. In the liver and kidney, no fragments could be
detected. Remarkably, 13 of the 16 patients with metastasized cancer showed pronounced accumulation of M30-positive fragments
containing hematoxylin-reactive material in the red pulp of the spleen. In the non-cancerous cases, such DNA-containing fragments
were only seen in 9 of 94 cases. The results show that when apoptotic activity is high, as during development in the fetus
or during metastasis and other pathological processes in the adult, the phagocytic clearance of apoptotic bodies can be overloaded.
These apoptotic fragments then accumulate in the spleen. The visual detection of apoptotic fragments is concluded to reflect
increased cell turnover.
Received 1 July 2002; accepted 1 July 2002 相似文献
959.
Strömberg P Svensson S Hedberg JJ Nordling E Höög JO 《Cellular and molecular life sciences : CMLS》2002,59(3):552-559
The human alcohol dehydrogenase system is comprised of multiple forms that catalyse the oxidation/reduction of a large variety of alcohols and aldehydes. A transition that results in an Ile308Val substitution was identified in the human ADH2 gene by single-strand conformation polymorphism analysis. Screening a Swedish population revealed that Val308 was the most frequent allele (73%), and site-directed mutagenesis was used to obtain both allelozymes, which were expressed in Escherichia coli for characterisation. Thermostability was assayed by activity measurements and circular dichroism spectroscopy. The results showed that the 308Val substitution decreases protein stability, as compared to the Ile308 variant, an effect also demonstrated during prolonged storage. Ethanol, octanol, 12-hydroxydodecanoic acid and all-trans retinol were used as model substrates and, generally, slightly higher Km values were observed with Val at position 308. Finally, homology modelling, from mouse ADH2, further supported the decreased stability of the Val308 variant and located position 308 in the subunit interface of the molecule and in the vicinity of the active-site pocket entrance. In conclusion, the Ile308Val substitution represents a novel functional polymorphism within the human alcohol dehydrogenase gene cluster that may affect the metabolism of ethanol and other substrates. 相似文献
960.
Screening for differentially expressed genes is a straightforward approach to study the molecular basis for changes in gene
expression. Differential display analysis has been used by investigators in diverse fields of research since it was developed.
Differential display has also been the approach of choice to investigate changes in gene expression in response to various
biological challenges in invertebrates. We review the application of differential display analysis of gene expression in invertebrates,
and provide a specific example using this technique for novel gene discovery in the nematode Caenorhabditis elegans. 相似文献