A rapid and accurate 3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide colorimetric assay for quantification of bacteriocins with nisin as an example

The objective of this study is to propose a more accurate and faster MTT [3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide] colorimetric assay (MCA) for quantitative measurement of polypeptide bacteriocins in solutions with nisin as an example. After an initial incubation of nisin and indicator bacterium Micrococcus luteus NCIB 8166 in tubes, MTT was added for another incubation period. After that, nisin was quantified by estimating the number of viable bacteria based on measuring the amount of purple formazan produced by cleavage of yellow tetrazolium salt MTT. Then MCA was compared to a standard agar diffusion assay (ADA). The results suggested a high correlation coefficient (r ²=0.975±0.004) between optical density (OD) and the inhibitory effect of nisin on a bacterial strain Micrococcus luteus NCIB 8166 at a range of 0.125~32 IU/ml. The MCA described in this study was very quick. Quantification of nisin took only 7~8 h and the detection limit was at the level of 0.125 IU/ml when compared to 12 IU/ml and 24~28 h for ADA. The MCA provides an accurate and rapid method for quantification of nisin in solutions and is expected to be used for quantification of other antimicrobial substances.     

三种群捕食-食饵离散系统的持久性

研究了具有第Ⅱ类功能性反应的三种群捕食-食饵离散系统，得到了保证该系统持久性的充分条件．     

浅论医疗事业单位固定资产的规范化管理

剖析了医疗事业单位固定资产管理工作中存在的问题及其产生的原因,提出了医疗事业单位加强对固定资产进行规范化管理的若干建议。     

东露天铁路专线采空区治理对策研究

针对东露天铁路专线所经过的采空区特点,分析了典型采空区上覆岩层的稳定性和上覆岩层移动形式,提出该地区采空区的治理措施。     

ARP欺骗攻击的整网解决方案研究

随着各种病毒与木马程序的泛滥,ARP欺骗攻击在校园网中已经成为危害网络正常稳定运行的主要问题.本文在对ARP协议进行深入分析的基础上,提出一种全网设备联动实现整体防御ARP欺骗的可行方案.     

ADC的结构及其应用

介绍了目前流行的ADC的4种结构及其性能特点。并从其电路实现的复杂程度、采样速度和应用领域等方面进行了对比研究．     

动力总成悬置支架拓扑优化研究

采用结构拓扑优化方法,对动力总成悬置支架进行了优化.在最优拓扑结构的基础上进行结构修改后,一阶固有频率明显提高,而重量有所减小.从加强筋的优化结果来看,在平行于一阶弯曲振型方向上布置加强筋,可获得较好的效果.研究表明,在产品的概念设计阶段采用拓扑优化方法,能给产品的详细设计提供一个很好的基础.     

Cesp(E1,E2)的k一致凸性

文章讨论了Banach空间的k一致凸性在cesp(E1，E2)中的提升问题。     

干式切削技术及其冷却方式

干式切削技术是指在切削加工过程中不使用任何切削液的新的工艺方法,具有清洁环保、降低生产成本、机床结构紧凑、提高产品质量等特点。对干式切削加工的关键技术之一———风冷却方式进行了介绍和论述。     

斜拉桥钢索的振动与张力问题

本文用弦振动理论分析了斜拉桥钢索的简谐振动和强迫振动，结合对钢索在常温下振动频率的测量，计算了常温某一温度的张力．采用线性插值由该张力值推算出其它气温下钢索的张力，并与实际的测量结果作了比较．