Cloning and expression profiles of 15 genes encoding WRKY transcription factor in wheat (Triticum aestivem L.)

WRKY proteins are involved in various physiological processes, including biotic and abiotic stress responses, hormone responses and development. However, no systematic identification, expression and function analysis of WRKY genes in wheat were reported. In this study, we isolated 15 wheat cDNAs with complete open reading frame (ORF) encoding putative WRKY proteins using in silico cloning. Phylogenetic analysis indicated that the 15 wheat WRKY genes belonged to three major WRKY groups. Expression analysis revealed that most genes expressed drastically in leaf, except TaWRKY10 which expressed in crown intensively. Four genes were strongly up-regulated with the senescence of leaves. Eight genes were responsive to low temperature, high temperature, NaCl or PEG treatment. Moreover, differential expression patterns were also observed between wheat hybrid and its parents, and some genes were more responsive to PEG treatment in the hybrid. These results demonstrated that wheat WRKY genes are involved in leaf senescing and abiotic stresses. And the changed expression of these WRKY genes in hybrid might contribute to the heterosis by improving the stress tolerance in hybrids.     

Prediction of gas chromatographic retention indices of organophosphorus compounds by holographic QSRR

A new molecular structure representation, molecular hologram, is employed to investigate the quantitative relationship between gas chromatographic retention indices and molecular structures for 41 methylesterified organophosphorus compounds (OPs). The quantitative structure-retention relationship (QSRR) model has been constructed for GC-RI of the selected OPs through partial least squares regression, which shows high statistical quality and predictive value with non-cross validation correlation coefficient ...     

Estimation of Partially Linear Panel Data Models with Cross-Sectional Dependence

Journal of Systems Science and Complexity - This paper studies the estimation of the partially linear panel data models, allowing for cross-sectional dependence through a common factors structure....     

Impact of dissolved humic acid on the bioavailability of acenaphthene and chrysene assessed by membrane-based passive samplers

Dissolved organic carbon (DOC) is known to reduce the bioavailability of hydrophobic organic compounds (HOCs) in aqueous environments. This reduction occurs as a result of adsorption to DOC, apparently reducing the freely dissolved concentration of HOCs. In the present study, triolein-embedded cellulose acetate membrane (TECAM) and Japanese medaka (Oryzias latipes) were used to measure the uptake of acenaphthene and chrysene in the presence of commercial humic acid (HA) at different concentrations (0―15 mg C·L-1) under controlled laboratory conditions. Apparent uptake rate constants for PAHs in TECAM and medaka were compared and DOC-water partition coefficients (KDOCs) of two PAHs were calculated with different sampling methods by model fit. Results showed that HA present in water significantly reduced the uptake of PAHs in TECAM and medaka. The obtained values of log KDOC of acenaphthene and chrysene measured by TECAM were 4.63 and 5.83, respectively, whereas biologically determined values were 4.52 and 5.76, respectively. These log KDOC values were comparable to earlier published KDOCs toward commercial HA, thereby indicating that TECAM accumulated only the freely dissolved fraction of chemicals and uptake PAHs in a manner similar to that of fish. All these results suggested that the TECAM method can provide a good means for assessing the impact of DOC on bioavailability of PAHs in the aqueous environment.     

Transitional phenomenon of particle dispersion in gas-solid two-phase flows

Without using any turbulent model, direct numerical simulation of a three-dimensional gas-solid two-phase turbulent jet was performed by finite volume method. The effects on dispersion of particles with different Stokes numbers by the transitional behavior of turbulent structures were investigated. To produce high-resolution results and reduce the computation and storage, the fractional-step projection algorithm was used to solve the governing equations of gas phase fluid. The low-storage, three-order Runge-Kutta scheme was used for time integration. The governing equations of particles were solved in the Lagrangian framework. These numerical schemes were validated by the good agreement be-tween the statistical results of flow field and the related experimental data. In the study of particle dis-persion, it was found that the effects on particle dispersion by the spanwise vortex structures were prominent. The new behaviors of particle dispersion were also observed during the evolution of the flow field, i.e. the transitional phenomenon of particle dispersion occurs for the particles with small and intermediate Stokes numbers.     

外语院校人才培养模式改革刍议

所谓人才培养模式,是指高等学校根据人才培养的目标和质量标准,为大学生设计的知识、能力和素质结构以及怎样实现这种结构的方式。社会对高等学校人才培养模式的评估,主要是以其外显质量特征即高等学校毕业生的质量作为依据来评价毕业生群体能否很好地适应国家、社会和市场的需要,从而提高人才培养对社会的适应程度,提高人才培养与培养目标的符合程度。本文将以外语院校的教育教学模式为基准,对如何有效评估、建立一套与之相适应的人才培养模式尝试进行一番剖析与论证,以供探讨。     

High-precision U-series dating of Holocene corals from South China Sea by thermal ionization mass spectrometry (TIMS)

Using the isotopic diluting and thermal ionization mass spectrometric (TIMS) technique, the U-series dating procedure was established on the basis of measurement of national standard GBW04412. With this highly precise technique the Holocene corals from the South China Sea were dated. Comparison between this method and α-spectrometry was made. The results show that a thriving period of corals in the South China Sea appeared at 6 800-5 800 a BP, during which the paleosea level was the higher than at present in the Holocene.     

朝鲜崖柏生境及高生长规律分析

采用标准地调查法测定朝鲜崖柏的土壤特征、林下植被和高生长规律.结果表明:朝鲜崖柏生长于土层较薄且呈弱酸性的山地暗棕壤,不同标准地林下植被差异明显;不同标准地朝鲜崖柏树高的年生长量均呈逐年下降趋势.     

乳腺纤维瘤患者经乳晕切口手术及传统手术治疗的疗效及安全性对比分析

目的评价乳腺纤维瘤患者经不同术式治疗后的疗效及安全性.方法将选取的60例患者随机分为A,B两组,A组给予经乳晕切口手术,B组给予传统手术方法,对其术后效果进行评定.结果与B组比较,A组的总有效率明显提高,哺乳率明显优于前者(P0.05),两组复发率比较差异无统计学意义(P0.05).与B组比较,A组的手术及住院时间明显缩短,术中出血量明显减少(P0.05,P0.01).A组患者乳晕感觉正常,双侧乳房基本对称,手术瘢痕可接受程度明显高于B组(P0.05).两组术后瘢痕大小比较差异具有统计学意义(P0.05),血肿或瘀斑大小比较差异无统计学意义(P0.05).与B组比较,A组患者术后美容效果的满意度明显高于前者,并发症发生率明显降低(P0.05).结论经乳晕切口治疗乳腺纤维瘤的效果较好,能够有效减少切口瘢痕,使乳房外形美观,还可降低并发症、复发率,安全性较高,患者较为满意.     

Implementation of an efficient segregated algorithm-IDEAL on 3D collocated grid system

The segregated algorithm-IDEAL (inner doubly-iterative efficient algorithm for linked-equations) is an efficient and stable algorithm. In this algorithm, there exist inner doubly-iterative processes for pressure equation, which almost completely overcome two approximations in SIMPLE algorithm. Thus the coupling between velocity and pressure is fully guaranteed, greatly enhancing the convergence rate and stability of iteration process. In this paper, implementation of the IDEAL algorithm on a 3D collocated grid system is conducted. The interface velocity is calculated by the modified momentum interpolation method (MMIM), by which the converged result is independent of the under-relaxation factor. Finally, five three-dimensional incompressible fluid flow and heat transfer problems are provided to compare the convergence rate and robustness between the IDEAL and three other most widely-used algorithms (SIMPLER, SIMPLEC and PISO). By the comparison it can be concluded that the IDEAL algorithm is more robust and efficient than the three other algorithms. Supported by the Key Project of National Natural Science Foundation of China (Grant No. 50636050), Fundamental Projects of Research and Development in China (973) (Grant Nos. 2006CB601203 and 2007CB206902)