《文学理论》教学改革刍议——教学问卷调查分析

文学理论课程是中文系学生理解文学史、作品选的工具之一,也是中文系学生进入专业理论学习的入门课程,文学理论课程的教学效果直接影响着学生进行各种文学课程学习的结果,但是,传统的文学理论授课方式很难吸引学生注意力,所以必须进行教学改革以此了解学生心理,针对当前学生情况改善教学方法, 激发学生学习乐趣从而对理论学习产生兴趣。     

如何做好医疗保险统计工作

根据自身工作实际,分析了长治市医疗保险统计工作的现状,对进一步加强工作提出了几点建议。     

Immune responses of a designed HIV-1 DNA vaccine on rhesus monkeys

According to the UNAIDS/WHO report (http:// www.unaids.org/Epi2005/doc/report.html), there are about 40.3 million HIV/AIDS survivors, and the new HIV infection number amounts to about 4.9 million, while the death number is some 3.1 million in the world in…     

Construction and anti-tumor effects of recombinant fowlpox virus expressing Newcastle disease virus hemagglutinin-neuramidinase gene

Hemagglutinin-neuramidinase (HN), a Newcastle disease virus-derived protein, not only mediates receptor recognition but also possesses neuraminidase (NA) activity, the ability to cleave a component of those receptors, N-acetylneuraminic acid (NAcneu, sialic acid). It is known that this protein in mammalian species, including human beings, has interesting anti-neoplastic as well as immune stimulating properties. To explore the use of the HN gene in cancer gene therapy, we constructed a recombi-nant fowlpox virus expressing the HN protein (vFV-HN) and compared the anti-tumor activity of the recombinant virus with that of wild-type fowlpox virus (FPV) in vivo and in vitro. Here we found that although B16 cells were somewhat resistant to the basal cytotoxic effect of wild-type fowlpox virus, infection with vFV-HN caused a pronounced cytotoxic effect and, the survival of tumor-bearing mice immunized with vFV-HN was significantly increased compared with the survival of mice immunized with the FPV alone. Furthermore, the immunization of mice with vFV-HN elicited a B16 tumor-specific cytotoxic T lymphocyte (CTL) response and clonal expansion of both CD4 and CD8 T cell populations in vivo. In addition, T cells from lymph nodes of mice vaccinated with vFV-HN secreted high levels of the Th1 cytokine IL-2 and IFN-γ, indicating that the regression of tumor cells is related to a Th1-type dominant immune response. These results demonstrate that vaccination with vFV-HN may be a potential strategy for cancer gene therapy.     

Rational design of glycerol dehydratase： Swapping the genes encoding the subunits of glycerol dehydratase to improve enzymatic properties

1,3-propanediol (1,3-PD) is an important material for chemical industry,and there has been always much interest in the production of 1,3-PD using all possible routes. The genes encoding glyc-erol dehydratase (GDHt) from Citrobacter freundii,Klebsiella pneumoniae and metagenome were cloned and expressed in E. coli. All glycerol dehy-dratases but the one from metagenome could be detected to show enzyme activities. In order to im-prove the enzymatic properties of GDHts,the genes encoding α and β-γ subunits were cloned,and the enzyme characteristics were evolved by rational de-sign based on their 3D structures which were con-structed by homology modeling. Six heteroenzymes were obtained by swapping the α subunit genes of these three different-source-derived GDHts. The pH,thermal stability and Vmax of some heteroenzymes were dramatically improved by 2―5 times compared with the wild one (GDHtKP). The GDHt cloned from metagenome,originally proved to be with no enzyme activity,was converted into active enzyme by swap-ping its subunits with other different GDHts. In addi-tion,the effect of subtle 3D structural changes on the properties of the enzyme was also observed.     

A Fault Detection Mechanism in Erasure-Code Byzantine Fault-Tolerance Quorum

Fault-tolerance is increasingly significant for large-scale storage systems in which Byzantine failure of storage nodes may happen. Traditional Byzantine Quorum systems that tolerate Byzantine failures by using replication have two main limitations： low space-efficiency and static quorum variables. We propose an Erasure-code Byzantine Fault-tolerance Quorum that can provide high reliability with far lower storage overhead than replication by adopting erasure code as redundancy scheme. Through read/write operations of clients and diagnose operation of supervisor, our Quorum system can detect Byzantine nodes, and dynamically adjust system size and fault threshold. Simulation results show that our method improves performance for the Quorum with relatively small quorums.     

Password-Based Group Key Agreement Protocol for Client-Server Application

0 IntroductionWith the rapid advances of computer networks , moreand more users are connecting together to exchangelarge amounts of information and share useful resources .However ,the security issue is still a major gating factor fortheir full adoption; mainly due to many malicious adversariesresidingin networked environments will eavesdrop,interceptand modify the transmittedinformation.The client-server model , over past years , has enjoyedsignificant and continually increasing popularity in…     

知识型企业知识状态系统演变的PSO机理及其优化模型分析

在知识工作者个人知识挖掘与整个知识型企业知识目标的协同的基础上,知识型企业与知识工作者之间挖掘知识集,不断进行协调从而推动知识状态系统演变的过程与鸟群或鱼群捕食过程的社会行为相似。利用PSO信息处理及优化机理,分析知识型企业知识状态系统演变的知识挖掘、传递以及决策特征,研究知识型企业知识状态系统的PSO模式,为进一步研究知识型企业知识状态系统演变特点的管理理论奠定基础。     

一类反向混合单调算子解的存在唯一性

运用锥与半序理论和非对称迭代方法,讨论半序Banach空间一类反向混合单调算子方程解的存在唯一性,并给出了迭代序列收敛于解的误差估计,作为其应用着重讨论了非反向混合单调算子方程解的存在唯一性,所得结果改进和推广了混合单调算子方程某些已知相应结果.     

外语院校人才培养模式改革刍议

所谓人才培养模式,是指高等学校根据人才培养的目标和质量标准,为大学生设计的知识、能力和素质结构以及怎样实现这种结构的方式。社会对高等学校人才培养模式的评估,主要是以其外显质量特征即高等学校毕业生的质量作为依据来评价毕业生群体能否很好地适应国家、社会和市场的需要,从而提高人才培养对社会的适应程度,提高人才培养与培养目标的符合程度。本文将以外语院校的教育教学模式为基准,对如何有效评估、建立一套与之相适应的人才培养模式尝试进行一番剖析与论证,以供探讨。