Identification of susceptibility genes loci associated with type 2 diabetes

In this study, we selected 10 susceptible SNPs loci to investigate their contribution to susceptibility to type 2 diabetes in Han Chinese among Hubei population. We genotyped SNPs rs5219, rs1801282, rs1470579, rs1111875, rs1081661, rs7754840, rs4506565, rs13266634, rs4402960, and rs5643981 by using the method of polymerase chain reaction-ligase detection reaction (PCR-LDR). In a case-control study, we have genotyped the 10 candidate susceptibility SNP loci, and here, we reported that the SNP rs5219 in KCNJ1...     

Hydrothermal synthesis and photoluminescence behavior of CeO<Subscript>2</Subscript> nanowires with the aid of surfactant PVP

Well-crystalline CeO₂ nanowires were prepared via a surfactant-assisted hydrothermal process. Reaction temperature and reaction time were changed for the determination of optimal synthesis parameters. The as-obtained products were characterized by X-ray diffraction (XRD), high-resolution transmission electron microscopy (HRTEM), and field emission scanning electron microscopy (FESEM). The results show that single crystal CeO₂ nanowires with high yield and good uniformity can be obtained hydrothermally at 180°C for 12 h with the aid of 2.0 g surfactant (polyvinyl pyrrolidone, PVP). The role of PVP was then discussed and a possible growth mechanism was proposed. Moreover, room temperature photoluminescence (PL) spectra were obtained for these CeO₂ nanowires, which are believed to be related to the abundant defects in these nanostructures.     

优化教学过程提高数学教学质量

实施素质教育,课堂教学是主阵地,在小学教学课堂教学中,如何优化教学过程,提高教学质量,谈谈个人的浅见.     

中国民主党派第一人谭平山

谭平山是中国近代史上有建树、有影响的民主革命家.辛亥革命时,他追随孙中山加入同盟会；五四运动后,他在陈独秀的帮助下建立中共广东支部.在第一次国共合作中,在轰轰烈烈的农民运动中,在著名的南昌起义中,都留下了他不可磨灭的历史功绩.他组建中华革命党,创建三民主义同志联合会,组织中国国民党革命委员会,参加新政协,特别是在响应中共“五一”口号、开展新政协运动中,他的主张和影响为民革和各民主党派接受中国共产党领导、协商建国,发挥了积极的作用.     

施工期钢筋混凝土结构的安全分析与安全指标

随着近年建筑业的发展，施工期钢筋混凝土结构的安全问题备受社会关注。在施工过程中，对钢筋混凝土结构进行必要的安全分析显得尤为重要，安全分析应主要建立在以结构荷载、荷载效应和抗力以及合理计算的基础上。文章在建筑结构可靠性理论的基础上，研究并讨论了荷载、荷载效应及抗力的特点并进行必要的安全分析。     

《医药市场营销学》课程的教学设计探索

目的:为提升《医药市场营销学》课程教学水平提供参考;方法:根据学生培养目标,设计《医药市场营销学》课程教学思路,进行探索性研究。结果与结论:《医药市场营销学》课程应以能力培养为教学目标,采取多种教学方法,重视实践教学。     

氟代芳基碳酸酯与氨基甲酸酯中氢键的理论研究

运用 AIM理论在 B97D 水平上计算了2-丁炔-1，4-二基二（2，3，4，5，6-五氟苯基碳酸酯）1，2-丁炔-1，4-二基二（4-氟苯基碳酸酯）2及2-丁炔-1，4-二基二（2，3，4，5，6-五氟苯氨基甲酸酯）3中的分子间有机氟 C -H…F -C 氢键．这些分子间氢键为闭壳相互作用．尽管晶体1中 H…F 距离接近经典氢键，但是该有机氟氢键仍为弱氢键．NBO 分析表明，研究的有机氟 C -H…F -C 氢键存在明显的电荷转移，用电荷转移合理地解释了分子1采取顺式结构．     

A multiple-labelling method for cells using Au nanoparticles with different shapes

An important and difficult issue is simultaneously identifying the detailed locations of various molecules on the cell surface, as this identification requires a synergistic effect between more than one molecule in a living cell. Au nanoparticles (NPs) with different shapes can be readily recognised under low vacuum scanning electron microscopy (lvSEM). Anisotropic Au nanorods (NRs) possess unique surface plasmon resonance (SPR) properties, which can be further utilised for two photon luminescence (TPL) and other optical imaging techniques. In this paper, Au NRs and Au nanooctahedra (Au NOs) are introduced as biomarkers for ICAM-1 and Integrin β1. Combined with the advantages of lvSEM, this multiple-labelling method is a new method for studying the interactions between specific, functional molecules.     

Features of intervertebral disc degeneration in rat’s aging process

Objective: The age-related change is important part of degenerative disc disease. However, no appropriate animal model or objective evaluation index is available. This study aimed to investigate the features of intervertebral disc degeneration in aging process of rats. Methods: 22-month-old Sprague-Dawley (SD) rats were used as spontaneously occurring intervertebral disc degeneration models and 6-month-old rats as young controls. Expression of collagen types II and X was measured by immunohistochemistry. Degenerations of intervertebral discs were scored according to Miyamoto’s method. Numbers and areas of afferent vascular buds were measured. The thicknesses of non-calcified and calcified layers were measured and statistically analyzed. Results: There were less collagen type II expression and more collagen type X expression in the calcified layer of the cartilage endplates and nucleus pulposus in the rats of the aged group than in the young control. There were fewer and smaller afferent vascular buds in the rats of the aged group than in the young control group. The ratio of the non-calcified to the calcified layers in the rats of the aged group significantly decreased, compared with that of the young control group (P<0.01). Conclusion: Rats can spontaneously establish intervertebral disc age-related degeneration. The expression of collagen types II and X, numbers and areas of afferent vascular buds, the ratio of the non-calcified to the calcified layers, and water and glycosaminoglycan contents in the nucleus pulposus are sensitive indexes of intervertebral disc degeneration.     

基于RTCC原理的精确定时研究与应用

介绍了一种基于RTCC的精确定时原理与方法，通过对PIC单片机其参数合理的设置、程序结构的合理安排以及配套算法的合理应用，实现了误差为零的精确定时方法，弥补了PIC系列特别是低端无中断MCU的精确定时的空白．并将此方法成功应用于实际工业环境中，取得了满意的结果．