定常流中立管的多参数分叉

本文讨论立管在拉力、自重及定常流的影响下的非线性分叉问题.奇摄动法用于求解多个独立参数的摄动分叉解.获得了模拟非对称平衡的分叉曲面.文中同时研究了各分叉曲面的稳定性.     

TMS32010数字信号处理器的系统设计与应用

一、TMS32010主要特点 TMS32010是一种新型的数字处理芯片,内部有16×16的硬件乘法器、32比特字长的算逻运算单元ALU、144个数据寄存器以及移位寄存器、辅助寄存器、堆栈和指令计数器等。TMS32010封装在40腿的DIP中,它的寻址能力为4K,对外数据字长为16比特。 TMS32010共有60条指令,可归纳为6类,即累加器操作指令18条;辅助寄存器及数据器指针操作指令7条;程序分支指令13条;T寄存器、P寄存器及乘法指令8条;中断控制及状态寄存器控制指令9条;I/O及存贮器操作指令5条。TMS32010的机器周期为200ns。     

煤层底板采动影响的研究

本文应用相似材料模似方法,研究了煤层底板采动影响。对模型底板岩体进行了综合观测,得到了底板岩体位移及应力的变化规律;並且观测了底板岩体声速变化,用以评价岩体的完整性,指出了底板不同部位裂隙发展变化状况。     

Glioma cell activation by Alzheimer's peptide Abeta1-42, alpha1-antichymotrypsin,and their mixture

We compared the effects ofAlzheimer's peptide (Abeta1-42), a,-antichymotrypsin (ACT) and an ACT/Abeta1-42 mixture on human glioma DK-MG cells. The solution of Abeta (5 microM) formed by 2-h incubation at room temperature induced tumour necrosis factor-alpha (TNF-alpha) and interleukin (IL)-6 levels by 55 and 45%, respectively, and increased gelatinase B activity by 67%, while exposure of cells to the ACT/Abeta1-42 mixture (1:10 molar ratio ACT: Abeta1-42) under the same experimental conditions showed no effect on IL-6 levels or gelatinase B activity, but strongly induced TNF-alpha (by 190%), compared to the controls. Stimulation of the cells with Abeta1-42 alone, but not with ACT, increased by about 20% low-density lipoprotein (LDL) uptake and mRNA levels for LDL receptor and HMG-CoA reductase, while the ACT/Abeta1-42 mixture significantly increased LDL uptake (by 50%), up-regulated mRNA levels for LDL receptor and HMG-CoA reductase by 48 and 63%, respectively, and increased lipid accumulation by about 20-fold. These data suggest a possible new role for Abeta in Alzheimer's disease through its interaction with the inflammatory reactant, ACT.     

Spectroscopic investigation on the telomeric DNA base sequence repeat

Telomeres are protein-DNA complexes at the terminals of linear chromosomes, which protect chromosomal integrity and maintain cellular replicative capacity. From single-cell organisms to advanced animals and plants, structures and functions of telomeres are both very conservative. In cells of human and vertebral animals, telomeric DNA base sequences all are (TTAGGG)n. In the present work, we have obtained absorption and fluorescence spectra measured from seven synthesized oligonucleotides to simulate the telomeric DNA system and calculated their relative fluorescence quantum yields on which not only telomeric DNA characteristics are predicted but also possibly the shortened telomeric sequences during cell division are implied. Oligonucleotide 5′-TTAGGGTTAGGG holds a low relative fluorescence quantum yield and remarkable excitation energy innerconversion, which tallies with the telomeric sequence of (TTAGGG)n. This result shows that telomeric DNA has a strong non-radiative or innerconvertible capability.     

影响新疆阜康荒漠地区红砂光合日变化因素的分析

目的 研究新疆阜康荒漠区域优势植物红砂（Reaumuria soongorica）光合及其影响因子的日变化，分析蒸腾速率、光合有效辐射、空气温度、气孔导度、相对湿度、叶面温度、胞间CO2浓度和空气CO：浓度对光合速率的影响。方法利用CI-301 CO2气体分析仪，测定光合及其影响因子的日变化，并进行相关性和多重线性回归分析。结果光合速率日变化形成典型的双峰型；蒸腾速率的日变化曲线呈双峰型；光合有效辐射、空气温度日变化和叶面温度的日变化曲线相近，在午后均呈逐步降低趋势；气孔导度与生境相对湿度的日变化为先下降，然后保持在一定的水平上；水分利用率全天递减。空气CO2浓度、空气温度、相对湿度及叶面温度和气孔导度对红砂光合速率具有显著的影响。结论红砂光合速率日变化曲线形成典型的双峰型，温度是影响红砂光合特性的重要因子之一，气孔因素则是导致其光合速率下降的决定因素。     

Research on the magnetic material of Sm-Fe matrix nitrides

In this paper, the types of Sm-Fe matrix compounds and their correlations are introduced, and progress of research on the magnetic materials of Sm-Fe matrix nitrides is also reviewed. Possible research trends of future permanent magnetic materials of SmFe matrix nitrides are briefly predicted.     

汉语元音的非线性预测

最近研究表明非线性的局部模型是最精确的混沌时间序列预测方法之一．同时有研究表明汉语的产生过程包含非线性．传统的语音处理技术忽视了语音中可能存在的非线性结构，因而限制了处理能力的进一步提高．对汉语元音／a／和／u／的细致相空间分析结果表明，汉语元音的重构相空间有类似于混沌吸引子的结构．根据汉语元音发声过程的非线性特性，进一步对其进行相空间的局部线性预测分析，计算了预测误差随预测步长的关系曲线，讨论了嵌入维数对预测性能的影响，并且与传统的线性方法作了比较．结果表明，尽管非线性的局域线性预测法存在计算开销大的问题，但其预测视野要远大于线性预测法，总体预测性能明显优于线性预测法．     

柱前衍生反相高效液相色谱法同时测定植物络合素(PCn)等巯基化合物

介绍一种荧光试剂monobromobimane柱前衍生，反相高效液相色谱一荧光检测测定标准样品和植物组织中植物络合素(PCn)等巯基化合物的方法．结果表明，采用乙腈和0．1％三氟乙酸组成的二元梯度洗脱程序，标准混合液中PCn等5种巯基化合物15min内得到很好的分离．以谷胱甘肽为代表考察方法的精密度，在1．25～160ng/μL浓度范围内与峰高有较好的线性关系，相关系数r^2=0．9999．谷胱甘肽的回收率平均为97．64％，40d内的变异系数平均为2．21％．方法具有快速、灵敏、稳定性好、易于实施、同时测定PCn等多种巯基化合物等优点．通过该方法的应用，首次揭示Cd超积累植物东南景天对CA的超量积累和解毒与PCn无关．     

重组刺桐胰蛋白酶抑制剂的制备及活性鉴定

刺桐胰蛋白酶抑制剂(ETI)是一种Kunitz型胰蛋白酶抑制剂,能够抑制胰蛋白酶及组织型纤溶酶原激活剂的活性,因此ETI可以作为配体与固定相偶联用于亲和层析.利用化学合成及聚合酶链式反应(PCR)的方法获得ETI的编码基因,然后用NdeI/SacI酶切并插入载体pET25b ( )中构建重组表达质粒pET25b ( )/ETI .重组表达质粒转化大肠杆菌BL21(DE3)后,用IPTG诱导使重组ETI(rETI)过量表达形成包涵体.包涵体经过洗涤、复性及CM离子交换柱纯化,每升培养液可获得14 mgrETI ,其纯度超过92 %.活性测定表明,rETI对凝血酶及尿激酶的活性没有抑制作用,但能显著抑制胰蛋白酶、瑞替普酶及纤溶酶的活性,抑制常数Ki分别为5 .14×10-9,9 .4×10-8和2 .08×10-8mol/L.