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11.
The knockout mouse project 总被引:1,自引:0,他引:1
Austin CP Battey JF Bradley A Bucan M Capecchi M Collins FS Dove WF Duyk G Dymecki S Eppig JT Grieder FB Heintz N Hicks G Insel TR Joyner A Koller BH Lloyd KC Magnuson T Moore MW Nagy A Pollock JD Roses AD Sands AT Seed B Skarnes WC Snoddy J Soriano P Stewart DJ Stewart F Stillman B Varmus H Varticovski L Verma IM Vogt TF von Melchner H Witkowski J Woychik RP Wurst W Yancopoulos GD Young SG Zambrowicz B 《Nature genetics》2004,36(9):921-924
Mouse knockout technology provides a powerful means of elucidating gene function in vivo, and a publicly available genome-wide collection of mouse knockouts would be significantly enabling for biomedical discovery. To date, published knockouts exist for only about 10% of mouse genes. Furthermore, many of these are limited in utility because they have not been made or phenotyped in standardized ways, and many are not freely available to researchers. It is time to harness new technologies and efficiencies of production to mount a high-throughput international effort to produce and phenotype knockouts for all mouse genes, and place these resources into the public domain. 相似文献
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Genomic rearrangement in NEMO impairs NF-kappaB activation and is a cause of incontinentia pigmenti. The International Incontinentia Pigmenti (IP) Consortium 总被引:12,自引:0,他引:12
Smahi A Courtois G Vabres P Yamaoka S Heuertz S Munnich A Israël A Heiss NS Klauck SM Kioschis P Wiemann S Poustka A Esposito T Bardaro T Gianfrancesco F Ciccodicola A D'Urso M Woffendin H Jakins T Donnai D Stewart H Kenwrick SJ Aradhya S Yamagata T Levy M Lewis RA Nelson DL 《Nature》2000,405(6785):466-472
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A. W. Stewart 《Cellular and molecular life sciences : CMLS》1985,41(4):456-458
Summary Fluorometric recordings of NADH (nicotinamide adenine dinucleotide) were made on rabbit papillary muscles. The specimens were placed between the UV light source and a small window of the detection stage. As the muscle was moved over the window in a transverse direction, simultaneous measurement could be taken of transmitted UV light and fluorescent light for various thicknesses of tissue. It is concluded that a muscle thinckness of 0.65 mm, is optimal before absorption of the incident light decreases the fluorescence signal. 相似文献
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Construction of an improved baculovirus insecticide containing an insect-specific toxin gene 总被引:30,自引:0,他引:30
L M Stewart M Hirst M López Ferber A T Merryweather P J Cayley R D Possee 《Nature》1991,352(6330):85-88
Baculoviruses provide alternatives to chemicals for controlling insect pests and can be applied by spraying. Baculoviruses have a limited host range, but work relatively slowly. They are dissolved in the midgut of insect larvae to release infectious virions which enter gut epithelial cells and begin to replicate. Replication in other organs causes extensive tissue damage and eventually death. This process can take 4-5 days, but in the field may last for more than a week, allowing the larvae to feed for longer and thereby damaging the host plant. Baculovirus expression vectors expressing foreign genes, such as those for insect-specific toxins, hormones or enzymes, might alleviate this problem. We have now constructed a recombinant baculovirus derived from Autographa californica nuclear polyhedrosis virus containing an insect-specific neurotoxin from the venom of the North African (Algerian) scorpion, Androctonus australis Hector. The neurotoxin acts by causing specific modifications to the Na+ conductance of neurons, producing a presynaptic excitatory effect leading to paralysis and death; it has no effect in mice. Expression of the neurotoxin by the virus causes a reduction in the time required to kill the host insect. 相似文献
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Monosodium glutamate induces convulsive disorders in rats 总被引:3,自引:0,他引:3