Functional analysis of the ABA-responsive protein family in ABA and stress signal transduction in Arabidopsis

The phytohormone abscisic acid (ABA) plays an important role in plant growth and development, for example in seed dormancy and germination, as well as in plant responses to environmental stresses, such as drought and high salinity. Previous studies have shown that ABA regulates the expression of genes with an ABA-responsive element (ABRE) and their corresponding physiological responses. Bioinformatics analysis identified a GRAM domain-containing gene family that has a multiple ABRE cis-element, which was termed the ABA-responsive protein (ABR) family. To analyze the function of the ABR family, we identified homozygous T-DNA insertion mutants and constructed abr1, 2, 3 double mutants and triple mutant. The abr1, abr2 and abr3 single mutants showed a normal phenotype; however, the germination of seeds of the double mutants and triple mutant were insensitive to ABA, NaCl, mannitol and glucose. ABR1-GFP was distributed as a punctate structure in the cytosol and may be localized in the endomembrane system. The ABR2-GFP and ABR3-GFP proteins localized in the cytoplasm. In addition, ABR1, ABR2 and ABR3 were expressed in various tissues, and could be induced by several abiotic stresses, especially by ABA. The expressions of these genes were significantly suppressed in aba2, abi1 and abi2 null mutants. These results suggested that the ABR family may act downstream of ABI1 and ABI2 in the ABA signal transduction process in plants.     

浅谈高等数学中的化归思想

化归思想贯穿于高等数学全部内容，是主要的数学思想方法，文章介绍了化归思想的含义。并阐述了化归思想在高等数学的应用及其在教学过程中如何渗透。     

The Function and Development of Soybean Peptides

Soybean peptides are small molecules hydrolyzed soy protein, from three to six amino acid composition of the peptide mixture, inl000Da molecular weight below.Because it has a lot of good physical and chemical properties and physiological functions, in many areas has been widely used. This paper reviews the soybean peptide physical and chemical characteristics, physiological functions, technology and applications in the food industry.     

Editor’s note

The coastal zone spans both land and water, extending landward up to the highest water line that occurs on shore during storms and seaward to the point at which storm waves no longer move sediments on the seafloor. The specific contact line between land and sea is the shoreline and adjacent land is considered     

综合集成方法与专家挖掘

我们面临的一些科学和实际问题越来越复杂需要运用各种知识的综合和技术的集成,其中知识的综合尤其是将专家的活的经验和智慧挖出来更是人们所关注的.数据挖掘、文本挖掘和网上挖掘能依靠计算机和一些人工智能方法可以从大量数据、文本和网上信息中找出一些有用的规则和知识,但是对于样本较少、知识隐含的活的专家经验如何去挖掘就是专家挖掘要研究的内容.作者及其同事们在近10年的研究中形成了一些概念、方法,并在一些社会问题、中医专家经验传承等进行了应用和探索.     

城市生活垃圾焚烧中PCDD/Fs合成抑制物研究

为减少城市生活垃圾焚烧过程中二噁英(PCDD/Fs)的生成量和毒性当量,在实验室规模的焚烧炉上进行了城市生活垃圾与单质硫和氧化钙混烧的对比实验,结果表明:单质硫和氧化钙对城市生活垃圾焚烧过程中PCDD/Fs的生成及其毒性当量均具有显著的抑制作用,抑制率可达到80％以上；在抑制多氯代二苯并呋喃(PCDFs)生成方面,单质硫抑制率要比氧化钙高1.48％,但在抑制PCDFs毒性当量方面,氧化钙抑制率要比单质硫高5.37％;单质硫和氧化钙都能改变PCDD/Fs同系物的分布,且氧化钙更能抑制毒性强的五氯代二苯并呋喃的合成,抑制率达到89.84％.     

论高速公路沥青混凝土路面平整度

沥青路面平整度问题是高速公路普遍面临的问题之一.文章着重分析了影响高速公路沥青路面平整度的因素,并提出了提高高速公路沥青路面平整度的措施.     

Sequence analysis of the Microcystis aeruginosa FACHB-912 phytochrome gene supports positive selection in cyanobacteria

The phytochrome gene from Microcystis aeruginosa FACHB-912,isolated from algal blooms in Taihu Lake in China,was sequenced and found to code for a wavelength transition light receptor protein.The gene was composed of five distinct domains:a PAS fold domain,a GAF domain,a phytochrome domain,a His Kinase A(phosphoacceptor) domain,and a histidine kinase domain.Red-absorbing phytochrome,far-red-absorbing phytochrome,and photoconversion kinetics were monitored via the spectral characteristics of the protein.To detect positive selection,homologous sequences from cyanobacterium phytochrome genes were obtained from GenBank.Potential selective pressure was identified by phylogenetic analysis with maximum likelihood analyses.The frequency of the phytochrome gene being subjected to selective constraints suggests that positive selection is a potentially important mechanism that promotes the evolution of the cyanobacteria phytochrome gene.The sites identified in this study provide targets for further research on the structural-functional role of these residues,and on the correlation with the mechanism of algal blooms.     

中学课堂提问不公平问题浅议

课堂教学是实施教育的主要阵地,从某种意义上讲,课堂教学的公平性会直接影响着教育公平性及其实现程度。在中学阶段,课堂提问不公平的原因是多方面的,主要有学生课堂上的不配合、班级规模过大、升学任务繁重和中学课程评价体制不完善、个别老师的素质偏低等原因造成的。因此,为了实现课堂提问的公平性,必须提升教师的整体素质、和谐处理师生之间的关系、合理理解并改革现行的教学评价体制等。     

山区农村职业高中农学专业发展思路的探索

分析了山区农村职业高中农学专业的发展现状及优势，针对山区农村中等职业教育长期内外交困的局面，积极探索并提出发展的科学思路和应对策略。