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A Sobel T Heidmann J P Changeux 《Comptes rendus des séances de l'Académie des sciences. Série D, Sciences naturelles》1977,285(14):1255-1258
A protein is purified by differential centrifugation from membrane fragments rich in acetylcholine receptor prepared from Torpedo marmorata electric organ after dissolution by a mixture of non denaturing detergents. After polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulphate and Coomassie blue staining the purified protein yields a single band of apparent molecular weight 43,000. Spectroscopic experiments carried out in the absence of Ca++ and detergents reveal that the 43 K protein interacts with the fluorescent local anesthetic quinacrine and with the frog toxin histrionicotoxin (apparent KD : 7 X 10(-7) M) but not with carbamylcholine and the alpha toxin from N. nigricollis. 相似文献
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B. S. Sherman S. Leibowitz A. E. Sobel 《Cellular and molecular life sciences : CMLS》1968,24(10):1003-1004
Zusammenfassung Es wird gezeigt, dass bei Entfernung eines Teiles der wasserlöslichen Phase einer meta-stabilen, verkalkenden Lösung, Kalk- und Phosphorionen so angereichert werden, dass «kristallisierte Kerne» von Hydroxyapatit auftreten.
This work was supported in part by grant No. DE-02456-03 from the National Institute of Dental Research, National Institutes of Health and by contract No. 4234 (00) with the Office of Naval Research, Department of the Navy. 相似文献
This work was supported in part by grant No. DE-02456-03 from the National Institute of Dental Research, National Institutes of Health and by contract No. 4234 (00) with the Office of Naval Research, Department of the Navy. 相似文献
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Microtubules are cytoskeletal polymers of tubulin involved in many cellular functions. Their dynamic instability is controlled by numerous compounds and proteins, including colchicine and stathmin family proteins. The way in which microtubule instability is regulated at the molecular level has remained elusive, mainly because of the lack of appropriate structural data. Here, we present the structure, at 3.5 A resolution, of tubulin in complex with colchicine and with the stathmin-like domain (SLD) of RB3. It shows the interaction of RB3-SLD with two tubulin heterodimers in a curved complex capped by the SLD amino-terminal domain, which prevents the incorporation of the complexed tubulin into microtubules. A comparison with the structure of tubulin in protofilaments shows changes in the subunits of tubulin as it switches from its straight conformation to a curved one. These changes correlate with the loss of lateral contacts and provide a rationale for the rapid microtubule depolymerization characteristic of dynamic instability. Moreover, the tubulin-colchicine complex sheds light on the mechanism of colchicine's activity: we show that colchicine binds at a location where it prevents curved tubulin from adopting a straight structure, which inhibits assembly. 相似文献
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Zusammenfassung Schilddrüsenanlagen von Hühnerembryonen, in Ambinon-Medium kultiviert, entwickeln sich schneller und zeigen beschleunigte intrafollikuläre Kolloidbildung. Mit Thiourazil behandelte Kulturen liessen retardierte Follikelbildung und Kolloidbildung erkennen. 相似文献
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Zusammenfassung Mäuse wurden mit Kasein- und Streptokokkus-Antigen injiziert. In den immunisierten Tieren ist die Ausscheidungsgeschwindigkeit von i.v. injiziertem Albumin-I131 aus dem Plasma erniedrigt, und ebenso ist im Vergleich zu den Kontrolltieren der transvaskuläre Transport in die Haut reduziert. 相似文献
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Zusammenfassung Cortison verzögert das Wachstum von embryonalen Femur-Anlagen und hemmt die hypertrophische Entwicklung des Knorpelsin vitro, fördert aber die Differenzierung der gestreiften Myoblasten. 相似文献
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