全文获取类型
收费全文 | 474篇 |
免费 | 2篇 |
国内免费 | 3篇 |
专业分类
系统科学 | 34篇 |
丛书文集 | 1篇 |
教育与普及 | 1篇 |
理论与方法论 | 3篇 |
现状及发展 | 95篇 |
研究方法 | 97篇 |
综合类 | 234篇 |
自然研究 | 14篇 |
出版年
2021年 | 3篇 |
2020年 | 4篇 |
2018年 | 3篇 |
2017年 | 3篇 |
2016年 | 5篇 |
2015年 | 3篇 |
2014年 | 5篇 |
2013年 | 8篇 |
2012年 | 27篇 |
2011年 | 66篇 |
2010年 | 10篇 |
2009年 | 2篇 |
2008年 | 39篇 |
2007年 | 38篇 |
2006年 | 36篇 |
2005年 | 32篇 |
2004年 | 27篇 |
2003年 | 19篇 |
2002年 | 30篇 |
2001年 | 8篇 |
2000年 | 3篇 |
1999年 | 4篇 |
1998年 | 2篇 |
1996年 | 3篇 |
1995年 | 2篇 |
1994年 | 2篇 |
1992年 | 6篇 |
1987年 | 3篇 |
1985年 | 2篇 |
1984年 | 3篇 |
1983年 | 2篇 |
1982年 | 3篇 |
1980年 | 2篇 |
1978年 | 5篇 |
1977年 | 5篇 |
1976年 | 5篇 |
1975年 | 4篇 |
1974年 | 3篇 |
1973年 | 2篇 |
1972年 | 5篇 |
1971年 | 4篇 |
1970年 | 7篇 |
1969年 | 5篇 |
1968年 | 5篇 |
1967年 | 2篇 |
1966年 | 3篇 |
1965年 | 8篇 |
1963年 | 1篇 |
1962年 | 1篇 |
1954年 | 1篇 |
排序方式: 共有479条查询结果,搜索用时 46 毫秒
401.
Peacock CS Seeger K Harris D Murphy L Ruiz JC Quail MA Peters N Adlem E Tivey A Aslett M Kerhornou A Ivens A Fraser A Rajandream MA Carver T Norbertczak H Chillingworth T Hance Z Jagels K Moule S Ormond D Rutter S Squares R Whitehead S Rabbinowitsch E Arrowsmith C White B Thurston S Bringaud F Baldauf SL Faulconbridge A Jeffares D Depledge DP Oyola SO Hilley JD Brito LO Tosi LR Barrell B Cruz AK Mottram JC Smith DF Berriman M 《Nature genetics》2007,39(7):839-847
Leishmania parasites cause a broad spectrum of clinical disease. Here we report the sequencing of the genomes of two species of Leishmania: Leishmania infantum and Leishmania braziliensis. The comparison of these sequences with the published genome of Leishmania major reveals marked conservation of synteny and identifies only approximately 200 genes with a differential distribution between the three species. L. braziliensis, contrary to Leishmania species examined so far, possesses components of a putative RNA-mediated interference pathway, telomere-associated transposable elements and spliced leader-associated SLACS retrotransposons. We show that pseudogene formation and gene loss are the principal forces shaping the different genomes. Genes that are differentially distributed between the species encode proteins implicated in host-pathogen interactions and parasite survival in the macrophage. 相似文献
402.
Sebaihia M Wren BW Mullany P Fairweather NF Minton N Stabler R Thomson NR Roberts AP Cerdeño-Tárraga AM Wang H Holden MT Wright A Churcher C Quail MA Baker S Bason N Brooks K Chillingworth T Cronin A Davis P Dowd L Fraser A Feltwell T Hance Z Holroyd S Jagels K Moule S Mungall K Price C Rabbinowitsch E Sharp S Simmonds M Stevens K Unwin L Whithead S Dupuy B Dougan G Barrell B Parkhill J 《Nature genetics》2006,38(7):779-786
We determined the complete genome sequence of Clostridium difficile strain 630, a virulent and multidrug-resistant strain. Our analysis indicates that a large proportion (11%) of the genome consists of mobile genetic elements, mainly in the form of conjugative transposons. These mobile elements are putatively responsible for the acquisition by C. difficile of an extensive array of genes involved in antimicrobial resistance, virulence, host interaction and the production of surface structures. The metabolic capabilities encoded in the genome show multiple adaptations for survival and growth within the gut environment. The extreme genome variability was confirmed by whole-genome microarray analysis; it may reflect the organism's niche in the gut and should provide information on the evolution of virulence in this organism. 相似文献
403.
Gudmundsson J Sulem P Gudbjartsson DF Jonasson JG Masson G He H Jonasdottir A Sigurdsson A Stacey SN Johannsdottir H Helgadottir HT Li W Nagy R Ringel MD Kloos RT de Visser MC Plantinga TS den Heijer M Aguillo E Panadero A Prats E Garcia-Castaño A De Juan A Rivera F Walters GB Bjarnason H Tryggvadottir L Eyjolfsson GI Bjornsdottir US Holm H Olafsson I Kristjansson K Kristvinsson H Magnusson OT Thorleifsson G Gulcher JR Kong A Kiemeney LA Jonsson T Hjartarson H Mayordomo JI Netea-Maier RT 《Nature genetics》2012,44(3):319-322
To search for sequence variants conferring risk of nonmedullary thyroid cancer, we focused our analysis on 22 SNPs with a P < 5 × 10(-8) in a genome-wide association study on levels of thyroid stimulating hormone (TSH) in 27,758 Icelanders. Of those, rs965513 has previously been shown to associate with thyroid cancer. The remaining 21 SNPs were genotyped in 561 Icelandic individuals with thyroid cancer (cases) and up to 40,013 controls. Variants suggestively associated with thyroid cancer (P < 0.05) were genotyped in an additional 595 non-Icelandic cases and 2,604 controls. After combining the results, three variants were shown to associate with thyroid cancer: rs966423 on 2q35 (OR = 1.34; P(combined) = 1.3 × 10(-9)), rs2439302 on 8p12 (OR = 1.36; P(combined) = 2.0 × 10(-9)) and rs116909374 on 14q13.3 (OR = 2.09; P(combined) = 4.6 × 10(-11)), a region previously reported to contain an uncorrelated variant conferring risk of thyroid cancer. A strong association (P = 9.1 × 10(-91)) was observed between rs2439302 on 8p12 and expression of NRG1, which encodes the signaling protein neuregulin 1, in blood. 相似文献
404.
405.
Wang K Kan J Yuen ST Shi ST Chu KM Law S Chan TL Kan Z Chan AS Tsui WY Lee SP Ho SL Chan AK Cheng GH Roberts PC Rejto PA Gibson NW Pocalyko DJ Mao M Xu J Leung SY 《Nature genetics》2011,43(12):1219-1223
Gastric cancer is a heterogeneous disease with multiple environmental etiologies and alternative pathways of carcinogenesis. Beyond mutations in TP53, alterations in other genes or pathways account for only small subsets of the disease. We performed exome sequencing of 22 gastric cancer samples and identified previously unreported mutated genes and pathway alterations; in particular, we found genes involved in chromatin modification to be commonly mutated. A downstream validation study confirmed frequent inactivating mutations or protein deficiency of ARID1A, which encodes a member of the SWI-SNF chromatin remodeling family, in 83% of gastric cancers with microsatellite instability (MSI), 73% of those with Epstein-Barr virus (EBV) infection and 11% of those that were not infected with EBV and microsatellite stable (MSS). The mutation spectrum for ARID1A differs between molecular subtypes of gastric cancer, and mutation prevalence is negatively associated with mutations in TP53. Clinically, ARID1A alterations were associated with better prognosis in a stage-independent manner. These results reveal the genomic landscape, and highlight the importance of chromatin remodeling, in the molecular taxonomy of gastric cancer. 相似文献
406.
Girard SL Gauthier J Noreau A Xiong L Zhou S Jouan L Dionne-Laporte A Spiegelman D Henrion E Diallo O Thibodeau P Bachand I Bao JY Tong AH Lin CH Millet B Jaafari N Joober R Dion PA Lok S Krebs MO Rouleau GA 《Nature genetics》2011,43(9):860-863
Schizophrenia is a severe psychiatric disorder that profoundly affects cognitive, behavioral and emotional processes. The wide spectrum of symptoms and clinical variability in schizophrenia suggest a complex genetic etiology, which is consistent with the numerous loci thus far identified by linkage, copy number variation and association studies. Although schizophrenia heritability may be as high as ~80%, the genes responsible for much of this heritability remain to be identified. Here we sequenced the exomes of 14 schizophrenia probands and their parents. We identified 15 de novo mutations (DNMs) in eight probands, which is significantly more than expected considering the previously reported DNM rate. In addition, 4 of the 15 identified DNMs are nonsense mutations, which is more than what is expected by chance. Our study supports the notion that DNMs may account for some of the heritability reported for schizophrenia while providing a list of genes possibly involved in disease pathogenesis. 相似文献
407.
408.
Lemaire SA McDonald ML Guo DC Russell L Miller CC Johnson RJ Bekheirnia MR Franco LM Nguyen M Pyeritz RE Bavaria JE Devereux R Maslen C Holmes KW Eagle K Body SC Seidman C Seidman JG Isselbacher EM Bray M Coselli JS Estrera AL Safi HJ Belmont JW Leal SM Milewicz DM 《Nature genetics》2011,43(10):996-1000
Although thoracic aortic aneurysms and dissections (TAAD) can be inherited as a single-gene disorder, the genetic predisposition in the majority of affected people is poorly understood. In a multistage genome-wide association study (GWAS), we compared 765 individuals who had sporadic TAAD (STAAD) with 874 controls and identified common SNPs at a 15q21.1 locus that were associated with STAAD, with odds ratios of 1.6-1.8 that achieved genome-wide significance. We followed up 107 SNPs associated with STAAD with P < 1 × 10(-5) in the region, in two separate STAAD cohorts. The associated SNPs fall into a large region of linkage disequilibrium encompassing FBN1, which encodes fibrillin-1. FBN1 mutations cause Marfan syndrome, whose major cardiovascular complication is TAAD. This study shows that common genetic variants at 15q21.1 that probably act via FBN1 are associated with STAAD, suggesting a common pathogenesis of aortic disease in Marfan syndrome and STAAD. 相似文献
409.
Stacey SN Sulem P Jonasdottir A Masson G Gudmundsson J Gudbjartsson DF Magnusson OT Gudjonsson SA Sigurgeirsson B Thorisdottir K Ragnarsson R Benediktsdottir KR Nexø BA Tjønneland A Overvad K Rudnai P Gurzau E Koppova K Hemminki K Corredera C Fuentelsaz V Grasa P Navarrete S Fuertes F García-Prats MD Sanambrosio E Panadero A De Juan A Garcia A Rivera F Planelles D Soriano V Requena C Aben KK van Rossum MM Cremers RG van Oort IM van Spronsen DJ Schalken JA Peters WH Helfand BT Donovan JL 《Nature genetics》2011,43(11):1098-1103
To identify new risk variants for cutaneous basal cell carcinoma, we performed a genome-wide association study of 16 million SNPs identified through whole-genome sequencing of 457 Icelanders. We imputed genotypes for 41,675 Illumina SNP chip-typed Icelanders and their relatives. In the discovery phase, the strongest signal came from rs78378222[C] (odds ratio (OR) = 2.36, P = 5.2 × 10(-17)), which has a frequency of 0.0192 in the Icelandic population. We then confirmed this association in non-Icelandic samples (OR = 1.75, P = 0.0060; overall OR = 2.16, P = 2.2 × 10(-20)). rs78378222 is in the 3' untranslated region of TP53 and changes the AATAAA polyadenylation signal to AATACA, resulting in impaired 3'-end processing of TP53 mRNA. Investigation of other tumor types identified associations of this SNP with prostate cancer (OR = 1.44, P = 2.4 × 10(-6)), glioma (OR = 2.35, P = 1.0 × 10(-5)) and colorectal adenoma (OR = 1.39, P = 1.6 × 10(-4)). However, we observed no effect for breast cancer, a common Li-Fraumeni syndrome tumor (OR = 1.06, P = 0.57, 95% confidence interval 0.88-1.27). 相似文献
410.