约化及可积系统分类

本文在约化等价的意义上研究了约化群,G_R~[l]的结构;证明了G_R在规范变换下的不变性,得到了约化的一个等价分类;给出了证明约化方程规范等价的一种方法。     

Ⅱ级建筑方格网的布设

认为对于小面积厂区,建立Ⅱ级控制方格网时,在满足工程和有关规程要求的条件下,可以采用简单的测设方法和计算方法,并以相对精度作为衡量方格网精度的主要指标.     

仿真表自动生成系统CT的设计

本文介绍了一个仿真表的自动生成系统。该系统用对话方式询问用户有关打印输出的信息,自动生成仿真表并完成数据的打印输出。     

基于服务端密钥存储的网络计算机数字证书系统

为了解决在网络计算机系统中部署数字证书系统时存在的终端实体对私钥数据的存储需求和网络计算机无本地存储特性的矛盾,提出一种基于服务端密钥存储的网络计算机数字证书应用系统的设计与实现方案。该方案基于公钥基础设施体系架构,采用了随机数、"盐"和多轮迭代等手段保证系统的安全性,并且通过在客户端进行密钥生成和加解密操作的方式实现了系统的可扩展性。系统实现的结果表明:由于无需借助外部密钥存储设备,该系统在保证安全性和可扩展性的同时,实施复杂度和成本均降低。     

Energy-dependent regulation of cell structure by AMP-activated protein kinase

AMP-activated protein kinase (AMPK, also known as SNF1A) has been primarily studied as a metabolic regulator that is activated in response to energy deprivation. Although there is relatively ample information on the biochemical characteristics of AMPK, not enough data exist on the in vivo function of the kinase. Here, using the Drosophila model system, we generated the first animal model with no AMPK activity and discovered physiological functions of the kinase. Surprisingly, AMPK-null mutants were lethal with severe abnormalities in cell polarity and mitosis, similar to those of lkb1-null mutants. Constitutive activation of AMPK restored many of the phenotypes of lkb1-null mutants, suggesting that AMPK mediates the polarity- and mitosis-controlling functions of the LKB1 serine/threonine kinase. Interestingly, the regulatory site of non-muscle myosin regulatory light chain (MRLC; also known as MLC2) was directly phosphorylated by AMPK. Moreover, the phosphomimetic mutant of MRLC rescued the AMPK-null defects in cell polarity and mitosis, suggesting MRLC is a critical downstream target of AMPK. Furthermore, the activation of AMPK by energy deprivation was sufficient to cause dramatic changes in cell shape, inducing complete polarization and brush border formation in the human LS174T cell line, through the phosphorylation of MRLC. Taken together, our results demonstrate that AMPK has highly conserved roles across metazoan species not only in the control of metabolism, but also in the regulation of cellular structures.     

马铃薯淀粉的场流分离表征过程回收率研究

采用场流分离技术探究了马铃薯(Solanum tuberosum L.)淀粉分离表征过程中样品间以及样品与超滤膜的交联,评价了载液离子强度、样品进样量、交叉流流速和聚集时间对马铃薯淀粉回收率的影响,并对结果重复性进行了考察.结果表明:在载液(含5 mmol/L NaNO₃,3 mmol/L NaN₃)pH 7.00,样品进样量15 μg,交叉流流速1.0 mL/min,聚集时间2.5 min的条件下,马铃薯淀粉的回收率为(97.30±0.02)%,回转半径相对标准偏差为1.18%,分子摩尔质量的相对标准偏差为5.67%.