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751.
Nitrogen transfer in the arbuscular mycorrhizal symbiosis 总被引:11,自引:0,他引:11
Govindarajulu M Pfeffer PE Jin H Abubaker J Douds DD Allen JW Bücking H Lammers PJ Shachar-Hill Y 《Nature》2005,435(7043):819-823
Most land plants are symbiotic with arbuscular mycorrhizal fungi (AMF), which take up mineral nutrients from the soil and exchange them with plants for photosynthetically fixed carbon. This exchange is a significant factor in global nutrient cycles as well as in the ecology, evolution and physiology of plants. Despite its importance as a nutrient, very little is known about how AMF take up nitrogen and transfer it to their host plants. Here we report the results of stable isotope labelling experiments showing that inorganic nitrogen taken up by the fungus outside the roots is incorporated into amino acids, translocated from the extraradical to the intraradical mycelium as arginine, but transferred to the plant without carbon. Consistent with this mechanism, the genes of primary nitrogen assimilation are preferentially expressed in the extraradical tissues, whereas genes associated with arginine breakdown are more highly expressed in the intraradical mycelium. Strong changes in the expression of these genes in response to nitrogen availability and form also support the operation of this novel metabolic pathway in the arbuscular mycorrhizal symbiosis. 相似文献
752.
Sieburth D Ch'ng Q Dybbs M Tavazoie M Kennedy S Wang D Dupuy D Rual JF Hill DE Vidal M Ruvkun G Kaplan JM 《Nature》2005,436(7050):510-517
Chemical synapses are complex structures that mediate rapid intercellular signalling in the nervous system. Proteomic studies suggest that several hundred proteins will be found at synaptic specializations. Here we describe a systematic screen to identify genes required for the function or development of Caenorhabditis elegans neuromuscular junctions. A total of 185 genes were identified in an RNA interference screen for decreased acetylcholine secretion; 132 of these genes had not previously been implicated in synaptic transmission. Functional profiles for these genes were determined by comparing secretion defects observed after RNA interference under a variety of conditions. Hierarchical clustering identified groups of functionally related genes, including those involved in the synaptic vesicle cycle, neuropeptide signalling and responsiveness to phorbol esters. Twenty-four genes encoded proteins that were localized to presynaptic specializations. Loss-of-function mutations in 12 genes caused defects in presynaptic structure. 相似文献
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Cyranoski D 《Nature》2005,435(7044):861
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Nanotechnology: high-speed integrated nanowire circuits 总被引:1,自引:0,他引:1
Macroelectronic circuits made on substrates of glass or plastic could one day make computing devices ubiquitous owing to their light weight, flexibility and low cost. But these substrates deform at high temperatures so, until now, only semiconductors such as organics and amorphous silicon could be used, leading to poor performance. Here we present the use of low-temperature processes to integrate high-performance multi-nanowire transistors into logical inverters and fast ring oscillators on glass substrates. As well as potentially enabling powerful electronics to permeate all aspects of modern life, this advance could find application in devices such as low-cost radio-frequency tags and fully integrated high-refresh-rate displays. 相似文献
757.
Silbo Gomero is a whistled language that is a rare and endangered surrogate of Spanish, used by shepherds on the island of La Gomera in the Canary Islands for communication over long distances on difficult terrain. Here we show that areas of the brain normally associated with spoken-language function are also activated in proficient whistlers, but not in controls, when they are listening to Silbo Gomero. Our findings demonstrate that the language-processing regions of the human brain can adapt to a surprisingly wide range of signalling forms. 相似文献
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DNA barcoding a useful tool for taxonomists 总被引:2,自引:0,他引:2
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