Skeletal muscle capillary densities during reactive hyperemia

Summary Reactive hyperemia was induced in hindlimbs of rats by occlusion of the femoral artery. Using fluorescein dye as a peripheral vascular marker, we observed that there was an increase in the number of flowing capillaries supplying the muscle fibres following release of the occlusion. The results indicate that the number of flowing capillaries is not dependent on the duration of occlusion (2–10 min).     

A template for the rapid measurement of Rf-values in thin-layer chromatography

Zusammenfassung Eine Schablone zur direkten Messung von Rf-Werten auf Dünnschichtplatten wird beschrieben, deren Ausführung den Besonderheiten der Dünnschichtchromatographie angepasst ist. Mit Hilfe dieser Schablone konnte die Auswertung von Chromatogrammen vereinfacht und wesentlich beschleunigt werden.     

Purification of a nuclease from human serum

Summary The purification procedure for a nuclease from human serum is described. It includes ammonium sulfate precipitation, chromatography on DEAE-Sephadex and on Sephacryl-S 200, and preparative electrophoresis. The enzyme, purified about 2000-fold, is homogeneous in a sodium dodecyl sulfate electrophoretic system, where it has a mol. wt of 78,000. The pH optimum lies around pH 6.5; it is a sugar-nonspecific endonuclease.Acknowledgment. This work has been supported by a research grant from Stiftung Volkswagenwerk through the Akademie der Wissenschaften und der Literatur, Mainz. We thank Mrs R. Nehrbass and Mrs C. Wolpert for technical assistance.